HIV-2 CRF01_AB: First Circulating Recombinant Form of HIV-2

Ibe, Shiro; Yokomaku, Yoshiyuki; Shiino, Teiichiro; Tanaka, Rie; Hattori, Junko; Fujisaki, Seiichiro; Iwatani, Yoshinori; Mamiya, Naoto; Utsumi, Makoto; Kato, Shingo; Hamaguchi, Motohiro; Sugiura, Wataru

doi:10.1097/qai.0b013e3181dc98c1

Cited by 49 publications

(43 citation statements)

References 42 publications

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“…7 In HIV-2-infected individuals the presence of a circulating recombinant form (CRF), 01_AB, has been detected. 8,9 In Cuba, from 1986 to December 2013, a total of 20 individuals were serologically confirmed as positive for antibodies to HIV-2 in the AIDS Research Laboratory (National Reference Laboratory for Human Retroviruses). 10,11 However, there are no reports available about HIV -2 groups circulating in the Cuban population.…”

mentioning

confidence: 99%

Phylogenetic Analysis of Human Immunodeficiency Virus Type 2 Isolated from Cuban Individuals

Machado¹,

Díaz²,

Noa³

et al. 2014

AIDS Research and Human Retroviruses

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The presence of infection by human immunodeficiency virus type 2 (HIV-2) in Cuba has been previously documented. However, genetic information on the strains that circulate in the Cuban people is still unknown. The present work constitutes the first study concerning the phylogenetic relationship of HIV-2 Cuban isolates conducted on 13 Cuban patients who were diagnosed with HIV-2. The env sequences were analyzed for the construction of a phylogenetic tree with reference sequences of HIV-2. Phylogenetic analysis of the env gene showed that all the Cuban sequences clustered in group A of HIV-2. The analysis indicated several independent introductions of HIV-2 into Cuba. The results of the study will reinforce the program on the epidemiological surveillance of the infection in Cuba and make possible further molecular evolutionary studies.

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mentioning

confidence: 99%

Phylogenetic Analysis of Human Immunodeficiency Virus Type 2 Isolated from Cuban Individuals

Machado¹,

Díaz²,

Noa³

et al. 2014

AIDS Research and Human Retroviruses

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“…In Japan, a number of HIV-2 infection cases have been reported (10)(11)(12). Therefore, both HIV-1 and HIV-2 tests are currently performed for diagnosis.…”

Section: Introductionmentioning

confidence: 99%

“…HIV-2 is divided into 8 groups (A-H) (5-8); among these, groups A and B are epidemic, while cases of the other HIV-2 groups are rare (8,9). The recombinant HIV-2 CRF01_AB was recently identified (10).…”

Section: Introductionmentioning

confidence: 99%

Qualitative Real-Time PCR Assay for HIV-1 and HIV-2 RNA

et al. 2016

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“…HIV-2 infections are mainly confined to regions of West Africa where an estimated 1-2 million people are infected [1], [2]. However, infections have also been identified in Europe, Asia, the Caribbean and the United States [3], [4], [5]. The virus is characterized by a high degree of genetic diversity and has been classified into at least eight groups, A and B being the most common [6], [7].…”

Section: Introductionmentioning

confidence: 99%

Discrepant Amplification Results during the Development of an Assay Leads to Reclassification of Two AIDS Reagent Repository HIV-2 Isolates as HIV-1

et al. 2014

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The development and verification of HIV-2 assays depends in part on the availability of well-characterized samples, including those from reagent repositories. During the development of an HIV-2 RNA quantification assay, two HIV-2 viral isolates (CDC 301340 and CDC 301342) obtained from the NIAID AIDS Reagent and Reference Repository were not detected leading to an investigation. Two HIV-2 primers/probe sets of known performance in real-time viral RNA quantification assays, targeting different regions of the virus, also failed to generate RT-PCR products for these two isolates. These isolates were tested in the HIV-1 specific COBAS AmpliPrep/COBAS TaqMan HIV-1 Test v2.0 (Roche Molecular Diagnostics) and were quantified at high copy number. Other HIV-2 isolates tested were not amplified in the COBAS HIV-1 TaqMan assay. Furthermore, the discrepant isolates were highly reactive in an HIV-1 p24 antigen test while the other HIV-2 isolates showed very weak, if any, cross-reactivity with the HIV-1 p24 assay. Phylogenetic tree analysis of sequences from the protease-reverse transcriptase regions of the discrepant HIV-2 isolates mapped with HIV-1 Group M, Subtype CRF02_AG confirming these isolates were of HIV-1 origin and had been misclassified as HIV-2. The use of misclassified isolates in the verification of molecular and immunological assays can lead to misinterpretation of test results, misdirection of efforts into assay redesign and increased development costs. The results of this study were shared with the NIAID AIDS Reagent Program, leading to the reclassification of the two discrepant isolates as HIV-1.

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HIV-2 CRF01_AB: First Circulating Recombinant Form of HIV-2

Abstract: We recently identified HIV-2 CRF01_AB cases in Japan. This ectopic observation of the virus outside its original endemic area suggests an ongoing global spread of HIV-2 CRF01_AB.

Cited by 49 publications

References 42 publications

Phylogenetic Analysis of Human Immunodeficiency Virus Type 2 Isolated from Cuban Individuals

Phylogenetic Analysis of Human Immunodeficiency Virus Type 2 Isolated from Cuban Individuals

Qualitative Real-Time PCR Assay for HIV-1 and HIV-2 RNA

Discrepant Amplification Results during the Development of an Assay Leads to Reclassification of Two AIDS Reagent Repository HIV-2 Isolates as HIV-1

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