HIV-1 Antiviral Activity of Recombinant Natural Killer Cell Enhancing Factors, NKEF-A and NKEF-B, Members of the Peroxiredoxin Family

Geiben-Lynn, Ralf; Kursar, Mischo; Brown, Nancy V.; Addo, Marylyn M.; Shau, Hungyi; Lieberman, Judy; Luster, Andrew D.; Walker, Bruce D.

doi:10.1074/jbc.m209964200

Cited by 90 publications

(74 citation statements)

References 60 publications

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“…Moreover, results from RT-PCR analysis conducted on purified CD4 ϩ and CD8 ϩ T cells from each cohort confirmed that PRDX2 was mainly expressed in CD8 ϩ T cell subset and that the expression of IL-22 transcripts was exclusively observed in the CD4 ϩ T cell subset (results not shown). These results are in line with the literature data reporting that IL-22 is produced by activated CD4 ϩ CD45RO ϩ T lymphocyte subset and, to a certain extent, NK cells (23) and that PRDX2 is mainly produced by CD8 (21). In PBMC, as expected, the CD4-CD8 ratio from all HIV ϩ was inversed, as compared with that from the HC and EU (Tables I and II), whereas the population of memory CD4 ϩ /CD45RO ϩ T cells from EU was proportionally increased (data not shown), as had already been demonstrated before for this particular cohort of individuals (13).…”

Section: Activated T Cells From Eu Overexpress Transcripts Of Il-22 Asupporting

confidence: 93%

“…The presence of PRDX2 has been reported in long-term nonprogressors, where it exhibits a strong, direct anti-HIV-1 activity in vitro (21). In contrast, IL-22 acts as an innate immune inducer; and because of its target cells, the only possible anti-HIV-1 activity of this cytokine is indirectly exerted by its downstream acute phase products.…”

Section: Discussionmentioning

confidence: 99%

“…Soluble IL-22 levels from anti-CD3 and anti-CD28 mAb-activated freshly isolated PBMC and PRDX2 serum levels were assessed, respectively, using an ELISA from R&D Systems and an ELISA previously described (21).…”

Section: Measurements Of Soluble A-saa Il-22 and Peroxiredoxin II (mentioning

confidence: 99%

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IL-22 Participates in an Innate Anti-HIV-1 Host-Resistance Network through Acute-Phase Protein Induction

Missé¹,

Yssel

Trabattoni

et al. 2007

The Journal of Immunology

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Certain individuals are resistant to HIV-1 infection, despite repeated exposure to the virus. Although protection against HIV-1 infection in a small proportion of Caucasian individuals is associated with mutant alleles of the CCR5 HIV-1 coreceptor, the molecular mechanism underlying resistance in repeatedly HIV-1-exposed, uninfected individuals (EU) is unclear. In this study, we performed complementary transcriptome and proteome analyses on peripheral blood T cells, and plasma or serum from EU, their HIV-1-infected sexual partners, and healthy controls, all expressing wild-type CCR5. We report that activated T cells from EU overproduce several proteins involved in the innate immunity response, principally those including high levels of peroxiredoxin II, a NK-enhancing factor possessing strong anti-HIV activity, and IL-22, a cytokine involved in the production of acute-phase proteins such as the acute-phase serum amyloid A (A-SAA). Cell supernatants and serum levels of these proteins were up-regulated in EU. Moreover, a specific biomarker for EU detected in plasma was identified as an 8.6-kDa A-SAA cleavage product. Incubation of in vitro-generated myeloid immature dendritic cells with A-SAA resulted in CCR5 phosphorylation, down-regulation of CCR5 expression, and strongly decreased susceptibility of these cells to in vitro infection with a primary HIV-1 isolate. Taken together, these results suggest new correlates of EU protection and identify a cascade involving IL-22 and the acute phase protein pathway that is associated with innate host resistance to HIV infection.

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Section: Activated T Cells From Eu Overexpress Transcripts Of Il-22 Asupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

IL-22 Participates in an Innate Anti-HIV-1 Host-Resistance Network through Acute-Phase Protein Induction

Missé¹,

Yssel

Trabattoni

et al. 2007

The Journal of Immunology

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“…Expression of Prdx2 has been reported to be important for the maintenance of anti-viral activity in CD8 þ T cells. 41 The knockout of Prdx2 in mice has been shown to cause an increase in T cells and dendritic cells 42 and increases in Figure 5 Silencing of one gene (Bcl2) in CD8 þ T cells from tolerized mice does not effect expression of other selected genes known to be involved (or not) in suppressive capacity of CD8 þ Treg in our system. CD8 þ T cells were isolated from pCons-treated mice 1 week after pCons tolerization using antibody-specific microbeads from AUTOMACS.…”

Section: Discussionmentioning

confidence: 83%

Distinct gene signature revealed in white blood cells, CD4+ and CD8+ T cells in (NZBx NZW) F1 lupus mice after tolerization with anti-DNA Ig peptide

et al. 2010

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Tolerizing mice polygenically predisposed to lupus-like disease (NZB/NZW F1 females) with a peptide mimicking anti-DNA IgG sequences containing MHC class I and class II T cell determinants (pConsensus, pCons) results in protection from full-blown disease attributable in part to the induction of CD4 þ CD25 þ Foxp3 þ and CD8 þ Foxp3 þ regulatory T cells. We compared 45 000 murine genes in total white blood cells (WBC), CD4 þ T cells, and CD8 þ T cells from splenocytes of (NZBxNZW) F1 lupus-prone mice tolerized with pCons vs untreated naïve mice and found two-fold or greater differential expression for 448 WBC, 174 CD4, and 60 CD8 genes. We identified differentially expressed genes that played roles in the immune response and apoptosis. Using real-time PCR, we validated differential expression of selected genes (IFI202B, Bcl2, Foxp3, Trp-53, CCR7 and IFNar1) in the CD8 þ T cell microarray and determined expression of selected highly upregulated genes in different immune cell subsets. We also determined Smads expression in different immune cell subsets, including CD4 þ T cells and CD8 þ T cells, to detect the effects of TGF-b, known to be the major cytokine that accounts for the suppressive capacity of CD8 þ Treg in this system. Silencing of anti-apoptotic gene Bcl2 or interferon genes (IFI202b and IFNar1 in combination) in CD8 þ T cells from tolerized mice did not affect the expression of the other selected genes. However, silencing of Foxp3 reduced expression of Foxp3, Ifi202b and PD1-all of which are involved in the suppressive capacity of CD8 þ Treg in this model.

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“…NKEF-A and -B, later identified as PrxI and PrxII respectively [6], can be detected in a wide range of tissues [7,8]. The NKEF protein may be also involved in apoptosis [9,10], cell proliferation, differentiation [11,12] and anti-viral activity in vitro [13]. Although recombinant NKEF (rNKEF)-A and -B have similar levels of antioxidant function, only the reduced form of rNKEF-A can enhance NK cytotoxicity, which indicates that both the antioxidant and NK-enhancing functions of rNKEF-A and -B probably involve the cysteine residues of the proteins but are mediated by separate domains of the molecules [14].…”

Section: Introductionmentioning

confidence: 99%

Structure, organization and expression of common carp (Cyprinus carpio L.) NKEF-B gene

Huang

Gao

Wang

et al. 2009

Fish & Shellfish Immunology

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HIV-1 Antiviral Activity of Recombinant Natural Killer Cell Enhancing Factors, NKEF-A and NKEF-B, Members of the Peroxiredoxin Family

Cited by 90 publications

References 60 publications

IL-22 Participates in an Innate Anti-HIV-1 Host-Resistance Network through Acute-Phase Protein Induction

IL-22 Participates in an Innate Anti-HIV-1 Host-Resistance Network through Acute-Phase Protein Induction

Distinct gene signature revealed in white blood cells, CD4+ and CD8+ T cells in (NZBx NZW) F1 lupus mice after tolerization with anti-DNA Ig peptide

Structure, organization and expression of common carp (Cyprinus carpio L.) NKEF-B gene

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