The response of primary dendrite spacing to a change in solidification condition is examined through the directional solidification of a succinonitrile-acetone alloy. While the growth velocity was kept constant, the thermal gradient G was alternately varied between two extreme values. The measured average dendrite spacing is compared to a calculation involving a previously proposed model, revealing good correlation. The investigation results enable us to characterize some important features about dendrite spacing evolution: during alternately changing G, dendrite spacing varies along different paths; after changing G-variation direction at turning points, the incubation periods required for the initiation of variation are much longer than those at the initial stage; as G changes back to its initial state, cannot return to its original value, revealing an unclosed hysteresis loop for the -G diagram; and continuing the process of alternately changing G will result in the formation of a closed loop. This kind of hysteresis loop is repeatable and roughly the same for varying G in reverse sequences. Thus, becoming free of the effect of the initial state, varies in nearly the same way for the same change in process condition, regardless of the difference in previous history.