Historical evolution and current status of the taxonomy of genus Pseudomonas

Peix, Álvaro; Ramírez-Bahena, Martha-Helena; Velázquez, Encarna

doi:10.1016/j.meegid.2009.08.001

Cited by 252 publications

(204 citation statements)

References 188 publications

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“…Two housekeeping genes, gyrB and rpoD, are frequently utilized as molecular markers to elaborate phylogenetic relationships within the genus Pseudomonas [48,[51][52][53]. Sequences of the POXN01 gyrB and rpoD genes were recovered from the obtained contigs of POXN01 genomic DNA and individually aligned with the homologous genes from type strains of 105 species belonging to genus Pseudomonas.…”

Section: Resultsmentioning

confidence: 99%

Isolation and molecular characterization of a novel <i>pseudomonas putida</i> strain capable of degrading organophosphate and aromatic compounds

Iyer¹,

Степанов²,

Iken³

2013

ABC

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A bacterial strain designated in this study as POXN01 was found to be capable of degrading the synthetic organophosphorus pesticides paraoxon and methyl parathion. The strain was initially isolated through enrichment technique from rice field soil near Harlingen, Texas. Phylogenetic analysis based on 16S rRNA, gyrB and rpoD gene alignments identified the POXN01 isolate as a new strain of Pseudomonas putida, which is closely related to the recently discovered nicotine-degrading strain Pseudomonas putida S16. While being unable to metabolize nicotine, the POXN01 isolate was observed to actively proliferate using monocyclic aromatic hydrocarbons, in particular toluene, as nutrients. Search for the genetic determinants of paraoxon catabolism revealed the presence of organophosphorus-degrading gene, opd, identical to the one from Sphingobium fuliginis (former Flavobacterium sp. ATCC 27551). Assimilation of aromatic compounds likely relies on phc ARKLMNOPQ gene cluster for phenol, benzene and toluene catabolism, and on benRABCDKGEF cluster for benzoate catabolism. The observed versatility of POXN01 strain in degradation of xenobiotics makes it useful for the multi-purpose bioremediation of contaminated sites in both agricultural and industrial environmental settings.

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Section: Resultsmentioning

confidence: 99%

Isolation and molecular characterization of a novel <i>pseudomonas putida</i> strain capable of degrading organophosphate and aromatic compounds

Iyer¹,

Степанов²,

Iken³

2013

ABC

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“…Subsequent investigation of the WS phenotype identified partially-acetylated cellulose as the main matrix component of the biofilm. The pseudomonads are a highly diverse genus (see recent reviews by [13][14]), and biofilm-formation and celluloseexpression are now known to be common amongst the water, soil, plant-associated and Adapted from [2][3]. *, Also known as the acetic acid bacteria; † , Referred to here as the enteric bacteria.…”

Section: Introductionmentioning

confidence: 99%

Cellulose Expression in Pseudomonas fluorescens SBW25 and Other Environmental Pseudomonads

Folorunso¹,

Zawadzki²,

Deeni³

et al. 2013

Cellulose - Medical, Pharmaceutical and Electronic Applications

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“…Comparatively little is known about the breakdown of the pesticide paraoxon in the environment or the microbial species responsible for its degradation. The most common molecular technique used to identify microorganisms is 16S rRNA sequencing [26][27][28][29]. However, species of larger genera, notably Pseudomonas, have proven difficult to fully resolve through this method alone [26][27][28]30].…”

Section: Introductionmentioning

confidence: 99%

“…The most common molecular technique used to identify microorganisms is 16S rRNA sequencing [26][27][28][29]. However, species of larger genera, notably Pseudomonas, have proven difficult to fully resolve through this method alone [26][27][28]30]. Secondary approaches to microbial population identification have included biochemical or physiological criteria that are then used to generate profiles that can be stored in databases for future reference.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Molecular and Biochemical Identification of Paraoxon-Metabolizing Pseudomonas Species

Iyer¹,

Iken²,

Tamez³

2011

J Bioremed Biodegrad

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Seven soil borne bacterial isolates were collected from locations throughout the Houston metropolitan area. All isolates proved capable of degrading the pesticide paraoxon to p-nitrophenol in carbon limited media and showed a high degree of tolerance to an environment exposed to increasing concentrations of the pesticide. A combination of 16S rRNA sequencing and fatty acid methyl ester (FAME) analysis was used to identify the unknown bacterial species. Universal bacterial primers were used for 16S rRNA analysis to tentatively identify all seven isolates as species belonging to the genus Pseudomonas. Sequencing results coupled with FAME analysis resolved the species identity of the seven isolates as either Pseudomonas aeruginosa or Pseudomonas putida. The results of this study demonstrate that a combination of molecular and biochemical analysis provide sufficient resolution to identify paraoxon degrading microbial populations.

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Historical evolution and current status of the taxonomy of genus Pseudomonas

Cited by 252 publications

References 188 publications

Isolation and molecular characterization of a novel <i>pseudomonas putida</i> strain capable of degrading organophosphate and aromatic compounds

Isolation and molecular characterization of a novel <i>pseudomonas putida</i> strain capable of degrading organophosphate and aromatic compounds

Cellulose Expression in Pseudomonas fluorescens SBW25 and Other Environmental Pseudomonads

Isolation, Molecular and Biochemical Identification of Paraoxon-Metabolizing Pseudomonas Species

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Historical evolution and current status of the taxonomy of genus Pseudomonas

Cited by 252 publications

References 188 publications

Isolation and molecular characterization of a novel &lt;i&gt;pseudomonas putida&lt;/i&gt; strain capable of degrading organophosphate and aromatic compounds

Isolation and molecular characterization of a novel &lt;i&gt;pseudomonas putida&lt;/i&gt; strain capable of degrading organophosphate and aromatic compounds

Cellulose Expression in Pseudomonas fluorescens SBW25 and Other Environmental Pseudomonads

Isolation, Molecular and Biochemical Identification of Paraoxon-Metabolizing Pseudomonas Species

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Isolation and molecular characterization of a novel <i>pseudomonas putida</i> strain capable of degrading organophosphate and aromatic compounds

Isolation and molecular characterization of a novel <i>pseudomonas putida</i> strain capable of degrading organophosphate and aromatic compounds