Cellulose Expression in Pseudomonas fluorescens SBW25 and Other Environmental Pseudomonads

Folorunso, Ayorinde O.; Zawadzki, K.; Deeni, Yusuf Y.; Koza, Anna; Moshynets, Olena

doi:10.5772/53736

Cited by 6 publications

(1 citation statement)

References 92 publications

(140 reference statements)

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“…Another set of potential cellulases was classified to the Pseudomonadales, an order including core adult-enriched Ventosimonas symbionts (Fig 1A; S8 Fig ) [58, 112]. But examination of the cellulase-associated gene cluster showed architectural similarities with the Pseudomonas fluorescens SBW25 wss cellulose-synthetase operon, documented previously [151] ( S20 Fig ). While the focal gene was, hence, likely a cellulase- encoding gene, the evidence did not support a digestive function for the predicted cellulase, but instead a role in the regulation of bacterial cellulose biosynthesis, which may be used in the formation of biofilms or in adherence to host tissues.…”

Section: Resultssupporting

confidence: 54%

In their sister’s footsteps: Taxonomic divergence obscures substantial functional overlap among the metabolically diverse symbiotic gut communities of adult and larval turtle ants

Béchade

Sanders

et al. 2021

Preprint

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Gut bacterial symbionts can support animal nutrition by facilitating digestion and providing valuable metabolites. While the composition of gut symbiont communities shifts with host development in holometabolous insects, changes in symbiotic roles between immature and adult stages are not well documented, especially in ants. Here, we explored the metabolic capabilities of microbiomes sampled from herbivorous turtle ant (Cephalotes sp.) larvae and adult workers through genomic and metagenomic screenings and targeted in vitro metabolic assays. We reveal that larval guts harbor bacterial symbionts from the Enterobacteriales, Lactobacillales and Rhizobiales orders, with impressive metabolic capabilities, including catabolism of plant and fungal recalcitrant fibers common in turtle ant diets, and energy-generating fermentation. Additionally, several members of the specialized turtle ant adult gut microbiome, sampled downstream of an anatomical barrier that dams large food particles, show a conserved potential to depolymerize many dietary fibers and other carbohydrates. Symbionts from both life stages have the genomic capacity to recycle nitrogen, synthesize amino acids and B-vitamins, and perform several key aspects of sulfur metabolism. We also document, for the first time in ants, an adult-associated Campylobacterales symbiont with an apparent capacity to anaerobically oxidize sulfide, reduce nitrate, and fix carbon dioxide. With help of their gut symbionts, including several bacteria likely acquired from the environment, turtle ant larvae appear as an important component of turtle ant colony digestion and nutrition. In addition, the conserved nature of the digestive, energy-generating, and nutritive capacities among adult-enriched symbionts suggests that nutritional ecology of turtle ant colonies has long been shaped by specialized, behaviorally-transferred gut bacteria with over 46 million years of residency.

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Section: Resultssupporting

confidence: 54%

In their sister’s footsteps: Taxonomic divergence obscures substantial functional overlap among the metabolically diverse symbiotic gut communities of adult and larval turtle ants

Béchade

Sanders

et al. 2021

Preprint

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Imaging Pseudomonas aeruginosa Biofilm Extracellular Polymer Scaffolds with Amphiphilic Carbon Dots

et al. 2016

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Biofilm formation is a critical facet of pathogenesis and resilience of human, animal, and plant bacteria. Extracellular polymeric substances (EPS) constitute the physical scaffolding for bacterial biofilms and thus play central roles in their development and virulence. We show that newly synthesized amphiphilic fluorescent carbon dots (C-dots) readily bind to the EPS scaffold of Pseudomonas aeruginosa, a major biofilm-forming pathogen, resulting in unprecedented microscopic visualization of the EPS structural features. Fluorescence microscopy analysis utilizing the C-dots reveals that the P. aeruginosa EPS matrix exhibits a remarkable dendritic morphology. The experiments further illuminate the growth kinetics of the EPS and the effect of external factors such as temperature. We also show that the amphiphilic C-dot platform enabled screening of substances disrupting biofilm development, specifically quorum sensing inhibitors.

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The Roles of the Various Cellulose Biosynthesis Operons in Komagataeibacter hansenii ATCC 23769

Bimmer

Mientus

Klingl

et al. 2022

Appl Environ Microbiol

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Cellulose Expression in Pseudomonas fluorescens SBW25 and Other Environmental Pseudomonads

Cited by 6 publications

References 92 publications

In their sister’s footsteps: Taxonomic divergence obscures substantial functional overlap among the metabolically diverse symbiotic gut communities of adult and larval turtle ants

In their sister’s footsteps: Taxonomic divergence obscures substantial functional overlap among the metabolically diverse symbiotic gut communities of adult and larval turtle ants

Imaging Pseudomonas aeruginosa Biofilm Extracellular Polymer Scaffolds with Amphiphilic Carbon Dots

The Roles of the Various Cellulose Biosynthesis Operons in Komagataeibacter hansenii ATCC 23769

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