Imaging <i>Pseudomonas aeruginosa</i> Biofilm Extracellular Polymer Scaffolds with Amphiphilic Carbon Dots

Ritenberg, Margarita; Nandi, Sukhendu; Kolusheva, Sofiya; Dandela, Rambabu; Meijler, Michaël M.; Jelinek, Raz

doi:10.1021/acschembio.5b01000

Cited by 44 publications

(29 citation statements)

References 50 publications

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“…Although carbon dots have been successfully exploited in imaging biofilm matrix (Ritenberg et al, 2016 ) and biofilm-encased microorganisms (Lin et al, 2017 ), it is for the first time to report that carbon dots can inhibit E. coli biofilm formation. Moreover, the synthesis of CDs-LP only requires L. plantarum LLC-605 as the only raw material through one-step hydrothermal reaction, which is very facile and renewable.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, carbon dots usually possess varied surface functional groups and can be easily engineered by other drugs and ligands for the applications in organelle-targeted imaging and/or drug delivery (Yang et al, 2015 ; Zhang et al, 2015 ; Hua et al, 2017a ). For biofilm application, carbon dots have been successfully exploited to image biofilm matrix (Ritenberg et al, 2016 ) and biofilm-encased microorganisms (Lin et al, 2017 ). However, to the best of our knowledge, the utilization of carbon dots in combating biofilm has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Bacteria-Derived Carbon Dots Inhibit Biofilm Formation of Escherichia coli without Affecting Cell Growth

Lin

Chen

2018

Front. Microbiol.

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Biofilms are deleterious in many biomedical and industrial applications and prevention of their formation has been a pressing challenge. Here, carbon dots, CDs-LP that were easily synthesized from the biomass of Lactobacillus plantarum by one-step hydrothermal carbonization, were demonstrated to prevent biofilm formation of E. coli. CDs-LP did not thwart the growth of E. coli, indicating the anti-biofilm effect was not due to the bactericidal effect. Moreover, CDs-LP did not affect the growth of the animal cell AT II, showing low cytotoxicity, good safety and excellent biocompatibility. Therefore, CDs-LP could overcome the cytotoxicity issue found in many current antibiofilm agents. CDs-LP represent a new type of anti-biofilm materials, opening up a novel avenue to the development of biofilm treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacteria-Derived Carbon Dots Inhibit Biofilm Formation of Escherichia coli without Affecting Cell Growth

Lin

Chen

2018

Front. Microbiol.

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“…Biofilm formation is essential for colonization of the plastic by microorganisms and without them, plastic cannot be effectively degraded. SEM allows for the visualization of bacterial colonization and biofilm architecture including extracellular polymeric substance (EPS) deposits which are essential scaffolding for productive biofilms 21 . Both lipasepositive consortia and Isolate 10 were able to colonize and form biofilms on PET, to different extents ( Fig.…”

Section: Identification Of Lipase Positive and Consortia Isolatesmentioning

confidence: 99%

Pseudomonasisolates degrade and form biofilms on polyethylene terephthalate (PET) plastic

Vague

Chan

Roberts

et al. 2019

Preprint

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Bioaugmentation is a possible remediation strategy for the massive amounts of plastic waste in our oceans and landfills. For this study, soil samples were collected from petroleum polluted locations in the Houston, Texas area to isolate microorganisms capable of plastic degradation.Bacteria were propagated and screened for lipase activity, which has been associated with the bacterial degradation of some plastics to date. We identified three lipase-positive Pseudomonas species, and Bacillus cereus as part of two consortia, which we predict enhances biofilm formation and plastic degradation. Lipase-positive consortia bacteria were incubated alongside blank and E.coli controls with UV-irradiated polyethylene terephthalate (PET), high-density polyethylene (HDPE), or low-density polyethylene (LDPE) as sole sources of carbon. Surface degradation of PET plastic was quantified by changes in molecular vibrations by infrared spectroscopy. The bacteria formed biofilms on PET, observed by scanning electron microscopy, and induced molecular changes on the plastic surface, indicating the initial stages of plastic degradation. We also found molecular evidence that one of the Pseudomonas isolates degrades LDPE. To date, lipase positive Pseudomonas spp. degradation of PET has not been well described, and this work highlights the potential for using consortia of common soil bacteria to degrade plastic waste.

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“…Moreover, amphiphilic carbon dots (CDs) have been shown to penetrate the EPS layer of P. aeruginosa, allowing direct visualization of its architecture, growth and how external agents affect its inhibition. The hydrocarbon side chains of CDs dock to the EPS network resulting in making the EPS scaffold highly fluorescent [46]. In yet another study, QDs with two varied surface chemistry [−COOH and polyethylene glycol (PEG) modified] were analyzed for their mobility and distribution in P. aeruginosa PAO1 biofilms.…”

Section: Role Of Qds In Inhibiting Biofilm Formationmentioning

confidence: 99%

Quantum Dots-Based Nano-Coatings for Inhibition of Microbial Biofilms: A Mini Review

Priyadarshini¹,

Rawat²,

Bohidar³

2018

Nonmagnetic and Magnetic Quantum Dots

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Infection of implants by microbial biofilm is chiefly caused by Staphylococci, Pseudomonas andCandida species. The growth of microbes by forming biofilms offers them protection from antibiotics, drugs and host defense mechanisms. The eradication of biofilms from implants and medical devices is difficult because of the protection by the biofilm forming pathogenic microbes. Hence, researches are focused on development of antibiofilm materials, which are basically constituted of antimicrobial substances or antimicrobial coatings. Nanomaterialbased coatings offer a promising solution in this regard. Quantum dots (QDs) are the group of semiconductor nanoparticles with high photoluminescent properties compared to conventional organic fluorophores. Thus, drug-conjugated QDs can be a promising alternative for biofilm treatment, and these can serve as excellent alternatives for the mitigation of recalcitrant biomaterial-associated infections caused by resistant strains. Furthermore, their use as antibiofilm coating would avoid the dispersion of antimicrobial agents in the surrounding cells and tissues, thereby minimizing the risks of developing microbial resistivity.

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Imaging Pseudomonas aeruginosa Biofilm Extracellular Polymer Scaffolds with Amphiphilic Carbon Dots

Cited by 44 publications

References 50 publications

Bacteria-Derived Carbon Dots Inhibit Biofilm Formation of Escherichia coli without Affecting Cell Growth

Bacteria-Derived Carbon Dots Inhibit Biofilm Formation of Escherichia coli without Affecting Cell Growth

Pseudomonasisolates degrade and form biofilms on polyethylene terephthalate (PET) plastic

Quantum Dots-Based Nano-Coatings for Inhibition of Microbial Biofilms: A Mini Review

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