Histophysiology of polysaccharide and lipid reserves in various tissues of Littorina littorea exposed to sublethal concentrations of cadmium

Gil, Javier; Marigómez, J. A.; Angulo, E.

doi:10.1016/0742-8413(89)90126-6

Cited by 11 publications

(9 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Accordingly, the glycogen content in Daphnia magna was halved after exposure to 5 g/L Cd via water for 21 d [34]. Similar effects due to exposure to sublethal Cd concentrations were observed for diverse molluscs [33,40]. In the present study, glycogen levels also declined in specimens of the Cd30 and both Zn groups.…”

Section: Metal Effectssupporting

confidence: 85%

“…In accordance with the severe diet-specific differences in the carbohydrate levels of L. dispar larvae, metal-contaminated groups were only compared with the artificial diet-fed control group and with the higher concentration of each metal. Biochemical changes in the hemolymph and tissue due to Cd exposure are well documented in marine invertebrates [8,[31][32][33][34], but comparable studies on terrestrial invertebrates are rare and mostly restricted to whole body composition [35,36]. Usually, Metals affect sugar levels in gypsy moth larvae Environ.…”

Section: Metal Effectsmentioning

confidence: 99%

See 1 more Smart Citation

Metal‐supplemented diets alter carbohydrate levels in tissue and hemolymph of gypsy moth larvae (Lymantria Dispar, lymantriidae, lepidoptera)

Ortel

1996

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Abstract-Larvae of Lymantria dispar were exposed to two concentrations each of Cd, Pb, Cu, and Zn from hatching to day 3 of the fourth instar. The metals were applied via artificial diet (wheat germ diet); two control groups were reared on either an uncontaminated artificial diet (C) or on a natural diet (oak leaves, EF). High-pressure liquid chromatography (HPLC) was employed to analyze the hemolymph carbohydrates, whereas body glycogen and glucose were determined enzymatically. The results were analyzed with respect to diet-specific differences (oak leaves versus wheat germ diet) and metal exposure compared with the uncontaminated artificial diet. Hemolymph trehalose levels were higher in oak leaf-reared individuals than in those fed on the wheat germ diet (p Ͻ 0.01), whereas the opposite applied to the body glycogen and free glucose levels (p Ͻ 0.01). The average trehalose value of the control (C) (4.3 mg/ ml) was reduced by metal contamination, dependent on both the metal itself and the concentration (Cd, Cu, Zn; 1.4-3.3 mg/ml). Sorbitol was not detected in the hemolymph of EF specimens, whereas it occurred in all artificial diet-fed groups. Metal-and dosedependent differences in the hemolymph sorbitol levels were observed in the treatment groups, but not in the controls. Glycogen content increased in the low concentration of Cd, Pb, and Cu, whereas a decrease was observed for the low Cd and both Zn concentrations. Tissue free glucose was enhanced only in three of the metal groups. Generally, fresh and dry weights of larvae were reduced in all groups except the high Cu-contaminated one. The results may indicate that mass outbreaks of an important forest pest insect like L. dispar may be facilitated in metal-contaminated areas because parasitization success of antagonistic species may decline due to deterioration of nourishment within the metal-stressed host.

show abstract

Section: Metal Effectssupporting

confidence: 85%

Section: Metal Effectsmentioning

confidence: 99%

Metal‐supplemented diets alter carbohydrate levels in tissue and hemolymph of gypsy moth larvae (Lymantria Dispar, lymantriidae, lepidoptera)

Ortel

1996

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…Thus, the reduction of neutral lipids is probably caused by increased consumption of lipids, as a source of energy, in stressed organisms, in order to activate cellular processes of detoxification (Guerlet et al 2006). Alternatively, certain pollutants can strengthen the activity of lipases, thus helping in the split of lipids (Gil et al 1989). Fig.…”

Section: Discussionmentioning

confidence: 98%

Effects of organic pollutants on Eobania vermiculata measured with five biomarkers

Itziou

Dimitriadis

2012

Ecotoxicology

View full text Add to dashboard Cite

In the present study, the effect of organic pollution on land snails Eobania vermiculata was investigated. Five pollution biomarkers (neutral red retention assay, morphometry of lysosomes and neutral lipids, acetylcholinesterase activity and metallothioneins content), were applied on tissues of the land snails. The results showed intense differentiations between the snails treated with organic pollutants and the control ones, as indicated by the results obtained. Statistically significant correlations among the results obtained emphasize the usefulness of these biomarkers.

show abstract

“…So, these results indicate that histological alterations in the hepatopancreas reflect better, the toxic effects of the tested insecticide than the dosage of biochemical contents. This can be attributed to the fact that thiametoxam at low tested concentrations does not interfere with the energetic metabolism of hepatopancreatic cells, and more exactly, the biosynthesis and the biodegradation of energetic molecules (carbohydrates, lipids) or of proteins (Gil and al., 1989;Padmaja and Rao, 1994). Nevertheless, the reduction of the different constituents at the highest tested concentrations of thiametoxam could be caused by the alterations of tubular cell structures in general and it could especially be caused by the destruction of the basal and topical membranes.…”

Section: Discussionmentioning

confidence: 99%

Histological changes and biochemical parameters in the hepatopancreas of terrestrial gastropod Helix aspersa as biomarkers of neonicotinoid insecticide exposure

Hamlet¹,

Bensoltane²,

Mohamed³

et al. 2012

Afr. J. Biotechnol.

View full text Add to dashboard Cite

In this study, adult snails, Helix aspersa were used to estimate the effect of aneonicotinoid insecticide (thiametoxam) on biochemical parameters and histological changes in the hepatopancreas of this gastropod after a treatment of six weeks. During this period, snails were exposed by ingestion and contact to fresh lettuce leaves which were soaked with an insecticide solution. The thiametoxam test solutions were 0, 25, 50, 100 and 200 mg/L. The results of the biochemical dosages (total carbohydrates, total proteins and total lipids) showed significant decreases at two concentrations (100 and 200 mg/L) of thiametoxam. However, the histological examination of the hepatopancreas of the treated snails showed alterations as a response to all the treatments, and revealed the degeneration of the digestive tubules and the breakdown of the basement membrane in a dose-dependent manner, leading to a severe deterioration of the tissues in the concentration of 200 mg/L thiametoxam. The dosage of carbohydrates, lipids and proteins supported by the study of histological changes on the hepatopancreas of H. aspersa can be considered as potential biomarkers of exposure to thiametoxam.

show abstract

Histophysiology of polysaccharide and lipid reserves in various tissues of Littorina littorea exposed to sublethal concentrations of cadmium

Cited by 11 publications

References 24 publications

Metal‐supplemented diets alter carbohydrate levels in tissue and hemolymph of gypsy moth larvae (Lymantria Dispar, lymantriidae, lepidoptera)

Metal‐supplemented diets alter carbohydrate levels in tissue and hemolymph of gypsy moth larvae (Lymantria Dispar, lymantriidae, lepidoptera)

Effects of organic pollutants on Eobania vermiculata measured with five biomarkers

Histological changes and biochemical parameters in the hepatopancreas of terrestrial gastropod Helix aspersa as biomarkers of neonicotinoid insecticide exposure

Contact Info

Product

Resources

About