Histopathological Study of the Effect of Guided Tissue Regeneration(GTR) with Atelocollagen-Natural Hydroxyapatite Composite.

Abstract

The purpose of this study is to evaluate the effects of Guided Tissue Regeneration (GTR) with biodegradable collagen membrane (Tissue GuideTM) and natural apatite composition materials (Bone Ject (R)) for experimental periodontal bone defect. The mesial roots of the lower third and fourth premolars from 10 beagle dogs were used. Mucoperiosteal flaps were reflected and bone defects measuring 5mm in width and 5mm in height were created. The experimental bone defects were treated by four different methods : (1) flap operation (control) , (2) GTR with biodegradable collagen membrane (T group) , (3) grafting Bone Ject (R) (B group) , (4) CI GTR with Tissue GuideTM and grafting Bone Ject (R) (TB group). After 24 and 48 weeks of healing, histologic sections were processed and histologically observed, showing that the TB group produced more new bone and new cementum than the other groups. When granules of Bone Ject (R) remained, the structure was observed smaller. Our study suggested that Bone Ject (R) reconstract a periodontal tissue while it is absorbed partially.