Histopathological Study of the Effect of Guided Tissue Regeneration(GTR) with Enamel Matrix Protein.

Abstract

The purpose of this study is to reconsider the attachment pattern and to evaluate the effects of Guided Tissue Regeneration (GTR) with Biodegradable collagen membrane (Tissue GuideTM) and enamel matrix protein (EMDOGAIN (R)) applied to experimental periodontal bone defects. The mesial roots of the lower third and fourth premolars from 10 beagle dogs were used. Mucoperiosteal flaps were reflected and bone defects were created measuring 5 mm in width and 5 mm in height. The experimental bone defects were treated by four different methods: (1) flap operation (control) ; (2) GTR with biodegradable collagen membrane (Tissue GuideTM) (T group) ; (3) applying enamel matrix protein (EMDOGAIN (R)) (E group) ; (4) GTR with Tissue GuideTM and EMDOGAIN (R) applied (TE group). After 6-12 weeks of healing, histologic sections were processed and histologically analyzed. In new cementum of T, E, and TE groups, hardly any cement cells were recognized, and no significant differences in attachment pattern among the three groups were noticeable. More significant new bone formation was found in the TE group than in the other groups, suggesting that GTR with Tissue GuideTM and EMDOGAIN (R) applyied may facilitate periodontal tissue regeneration. J.