Histopathological Kidney Overview of Experimental Diabetes Mellitus Wistar Rats Given Ethanol Extract of Moringa Leaf

Abstract

This study aims to prove that the Moringa leaf extract can improve renal histological structure of diabetic Wistar rats. Wistar rats were divided into six groups, so that each group has four replications. K1 as the positive control was not given the extract, K2 was administered the extract dose 100 mg/kg, K3 was administered the extract dose 200 mg/kg, K4 was administered the extract dose 300 mg/kg, K5 was administered the extract dose 400 mg/kg, K6 was administered the extract dose 500 mg/kg. The administrations of extract were held for five weeks. Rats then sacrificed and the kidneys were taken for the histopathology preparations with Hematoxylin-Eosin staining (HE). The observations indicate fatty degeneration and necrosis of the kidneys. Kruskal Wallis test results of all treatments showed p = 0.001 in both fatty degeneration and necrosis (p <0.05).

THE INHIBITION EFFECT OF MORINGA (Moringa oleifera L.) LEAVES AND RED GINGER RHIZOME (Zingiber officinale Rosc.) PT. "P" ETHANOL EXTRACT ON ENHANCEMENT SERUM CREATININE LEVELS OF MALE WISTAR RATS DYSLIPIDEMIA MODEL

“…This can happen because the 2 herbs used have an antioxidant effect with ascorbic acid, flavonoid, phenolic, and carotenoid content which work by preventing the generation of ROS and directly capturing ROS. 16,19 Another mechanism of action is changing free radicals. reactive free radicals become relatively stable reactive free radicals by giving hydrogen atoms to reactive free radicals to inhibit oxidation so that they can function as renoprotection.…”

“…14,15 The antioxidant effect of flavonoids can function as a renoprotection and antihyperlipidemic. 16,17 This study uses a human dose conversion of 3 x 500 mg/day as stated on the package.…”

“…Antioxidants can work by preventing the degeneration of ROS and directly capturing ROS so that they can reduce oxidative stress which can cause kidney damage. 16,19 In addition, antioxidants can convert reactive free radicals into reactive free radicals that are more stable by donating hydrogen atoms to reactive free radicals so that they can inhibit oxidation. 20 Antihyperlipidemic in Moringa leaves works by inhibiting the activity of the enzyme Acyl-CoA cholesterol acyltransferase (ACAT) and 3-hydroxy-3-methylglutaryl CoA reductase on HepG2.…”

UJI TOKSISITAS AKUT EKSTRAK KUNYIT PUTIH (Curcuma zedoaria) PADA GINJAL TIKUS WISTAR JANTAN

“…15 Proses degenerasi timbul oleh proses kerusakan tubulus ginjal, terutama saat akumulasi ekstrak kunyit putih yang tinggi pada tubulus proksimal secara terus menerus pada absorbsi dan sekresi. 16 Saat konsentrasi suatu senyawa yang diberikan semakin tinggi, ginjal akan mulai memperlihatkan respons berupa degenerasi tubulus pada gambaran mikroskopis. Degenerasi ditandai adanya kondisi gangguan metabolik dimana sel mengalami kehilangan sruktur normalnya sehingga menimbulkan penimbunan bahan-bahan baik secara ekstraseluler maupun intraseluler.…”

“…17 Pemberian ekstrak bila dilanjutkan dalam waktu yang lama, degenerasi dapat berlanjut menjadi nekrosis dan menyebabkan penurunan fungsi ginjal. 16 Maka dari itu perlu dilakukan pengujian toksisitas pemberian ekstrak kunyit putih lebih lanjut terhadap ginjal dalam periode yang lebih lama.…”