Histopathological evaluation of bivalves from the southwest coast of India as an indicator of environmental quality

Joshy, Aswathy; Sharma, S R Krupesha; Mini, K G; Gangadharan, Suja; Pranav, P.

doi:10.1016/j.aquatox.2022.106076

Cited by 25 publications

(5 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Destructive reactions at the expense of gill tissue morphology and functions, such as vacuolation, lamellar fusion, and loss of epithelial morphology, were already reported by several authors in bivalve molluscs, as common reactions to pollutant exposure ( Khudhur et al, 2019 ; Joshy et al, 2022 ; Khan et al, 2018 ). The influence of ASA concentration on these modifications has followed a constantly increasing trend during our study.…”

Section: Discussionmentioning

confidence: 75%

Toxicological Evaluation of Acetylsalicylic Acid in Non-Target Organisms: Chronic Exposure on Mytilus galloprovincialis (Lamarck, 1819)

et al. 2022

View full text Add to dashboard Cite

Pharmaceuticals are now considered to be established contaminants, and their presence in water poses a real risk not only to the marine ecosystem, as they may adversely affect non-target organisms that are exposed to them, but also indirectly to humans. This is particularly true for the model organism considered in this work, Mytilus galloprovincialis (Lamarck, 1819), a suspensivore and bioaccumulating organism that enters the human food chain. Among the most commonly used over-the-counter medicines, anti-inflammatory drugs certainly feature prominently, with acetylsalicylic acid (ASA) at the top. In this work, M. galloprovincialis specimens were exposed to two concentrations of ASA (10 and 100 μg/L) for 10 and 20 days to evaluate possible alterations in the decrease in regulatory volume (RVD) in digestive gland cells and cell viability of both these cells and hemocytes. In addition, the histopathological condition index of the gills and digestive gland was evaluated. The data obtained showed that chronic exposure to ASA did not alter the cell viability of hemocytes and digestive gland cells but alters the physiological mechanisms of volume regulation in the digestive gland and, in addition, a time-dose reaction to ASA in the gills and digestive gland showing numerous alterations such as lipofuscin deposits and hemocyte infiltration was found. These results confirm the potential toxicity to the marine biota, highlighting the necessity to deepen the knowledge regarding the link between over-the-counter pharmaceuticals and non-target organisms.

show abstract

Section: Discussionmentioning

confidence: 75%

Toxicological Evaluation of Acetylsalicylic Acid in Non-Target Organisms: Chronic Exposure on Mytilus galloprovincialis (Lamarck, 1819)

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Finally, each stained slice was photographed using Pannoramic MIDI slide scanner (3DHISTECH, Budapest, Hungary) and analyzed using the Case-Viewer software (3DHISTECH, Budapest, Hungary). The scoring methods developed and modified by Bernet et al (1999), Costa et al (2013), and Joshyd et al (2022) were used to assess the histopathological condition indices (HPI) in bivalves [ 49 , 58 , 59 ].…”

Section: Methodsmentioning

confidence: 99%

Possible Role of Docosahexaenoic Acid in Response to Diarrhetic Shellfish Toxins in the Mussel Perna viridis

et al. 2023

View full text Add to dashboard Cite

Marine bivalves are rich in docosahexaenoic acid (DHA), a polyunsaturated fatty acid known to be beneficial for human health; however, the potential role of DHA in protecting shellfish from the toxicity of diarrhetic shellfish toxins (DSTs) remains poorly understood. Here, we aimed to study the effect of DHA on the response of the bivalve, Perna viridis, to DSTs by using LC-MS/MS, RT-qPCR, and histological examination. In this study, we observed that the DHA content decreased significantly with esterification of DSTs in the digestive gland of the mussel P. viridis after 96 h of exposure to Prorocentrum lima, a DST-producing dinoflagellate. The addition of DHA significantly increased the esterification level of DSTs and increased the expression of Nrf2 signaling pathway-related genes and enzyme activities, alleviating the damage of DSTs to digestive glands. These results suggested that DHA may mediate the esterification of DSTs and activation of the Nrf2 signaling pathway in P. viridis to protect mussels from the toxic effects of DSTs. This study may provide new insights regarding the response of bivalves to DSTs and lay the foundation for uncovering the role of DHA in environmental adaptation of bivalves.

show abstract

“…Hemocytes were shown to perform osmoregulatory responses during the salinity stress and are convenient model objects for investigation of the intracellular ROS metabolism (Kladchenko et al, 2022, Lignot et al, 2000. Gills were selected for the present study due to their role respiration and osmoregulation and because this tissue directly contacts the surrounding aquatic environment (Joshy et al, 2022). The range of experimental salinity corresponded to naturally observed salinity fluctuations in coastal aquatic systems of the Black Sea.…”

Section: Introductionmentioning

confidence: 99%

Short-time salinity fluctuations are strong activators of oxidative stress in Mediterranean mussel (Mytilus galloprovincialis)

Gostyukhina,

Kladchenko,

Chelebieva

et al. 2023

Ecol. Monten.

View full text Add to dashboard Cite

Salinity stress is an emerging key factor affecting aquatic animals in coastal waters. In bivalves, physiological responses to anisosmotic conditions are frequently associated with excessive reactive oxygen species (ROS) formation, lipid peroxidation processes and alterations of the antioxidant enzyme activity in tissues. In this study, we investigated whether short-time exposure to high (20-40 ‰) or low (6-14 ‰) salinity induces oxidative stress in hemocytes and gills of Mediterranean mussel (Mytilus galloprovincialis). Our findings showed that both hypoosmotic and hyperosmotic stress enhanced formation of ROS in hemocytes, which was accompanied by an increase in DNA damage. Experimental acclimation to low salinity (6‰, 10 ‰) led to a decrease of the activity of superoxide dismutase but did not affect catalase activity in gills. Exposure to high salinity (24 ‰) inhibited activities of both antioxidant enzymes, whereas at 30 ‰ activity of both enzymes recovered up to controls. We found that exposure to high salinity was associated with lower risks of oxidative stress in mussels, and changes in redox balance are observed in hemocytes and gills even if the range of salinity changes is in range of species adaptive potential. Taken together, this study suggests that short-time salinity fluctuations promote oxidative stress in bivalves.

show abstract

Histopathological evaluation of bivalves from the southwest coast of India as an indicator of environmental quality

Cited by 25 publications

References 54 publications

Toxicological Evaluation of Acetylsalicylic Acid in Non-Target Organisms: Chronic Exposure on Mytilus galloprovincialis (Lamarck, 1819)

Toxicological Evaluation of Acetylsalicylic Acid in Non-Target Organisms: Chronic Exposure on Mytilus galloprovincialis (Lamarck, 1819)

Possible Role of Docosahexaenoic Acid in Response to Diarrhetic Shellfish Toxins in the Mussel Perna viridis

Short-time salinity fluctuations are strong activators of oxidative stress in Mediterranean mussel (Mytilus galloprovincialis)

Contact Info

Product

Resources

About