Histopathological detection of parasitic infections

Hemalatha, S.; Edith, R.; Sreekumar, C.; Arumugam, S.; Gomathinayagam, S.; Roy, Parimal; Ganesan, Prakash

doi:10.5958/0974-0813.2018.00006.2

Cited by 1 publication

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“…The diagnosis of parasite infections have been classically established by clinical data and morphological criteria by direct observation of tissue samples under the microscope 13 . Currently, the application of molecular-based techniques has modified the diagnostic process, as well as the classification and taxonomy of parasite species 12,14,15 , as in many cases morphological characteristics do not allow classification beyond the genus level, and new genotypes, some of them re-classified now as new species, have been recognized by applying new molecular and immunohistochemical techniques 12 .…”

Section: Introductionmentioning

confidence: 99%

Application of RGB trichrome staining to the study of human parasites

Gonzalez-Luna

Gaytán

2022

Preprint

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Human parasitic infections are major contributors to global disease load, compromising the human life and resulting in considerable morbidity and mortality worldwide. However, many parasitic diseases have been neglected and little investigated in western medicine. Although recently developed molecular techniques have revolutionized the taxonomy of parasites and the parasitic disease diagnosis, histopathology still remains a powerfull tool for the analysis of parasitic diseases, allowing direct observation of the parasite thus providing information about the morphological features of the parasite ifself, and revealing tissue alterations at the parasite-host interface. The recently developed RGB trichrome (acronym for the primary dye components, picrosirius Red, fast Green, and alcian Blue) stains the main components of the extracellular matrix, specifically collagens and proteoglycans. We have applied the RGB tricrome staining to human tissues infected by the main three classes of human parasites: Protozoa (Leishmania donovanii and Toxoplasma gondii), helminths (Trichinella spiralis, Enterobius vermicularis, Dirofilaria spp. and Echinococcus granulosus) and ectoparasites (Demodex folliculorum and Demodex brevis). Trichrome stain results in detailed staining of the parasite microanatomical structure, and highlights host tissue alterations such as granulomatous inflammation, immune cell infiltrate, or increased amount of collagen as a sign of parasite-induced fibrosis. Yet, the use of RGB trichrome, as a complement of hematoxylin and eosin staining, provides additional valuable information to assess parasitic infection histopathology.

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