Histopathological assessment and inflammatory response in the digestive gland of marine mussel Mytilus galloprovincialis exposed to cadmium-based quantum dots

Rocha, Thiago Lopes; Sabóia‐Morais, Simone Maria Teixeira de; Bebianno, Maria João

doi:10.1016/j.aquatox.2016.06.003

Cited by 59 publications

(16 citation statements)

References 44 publications

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“…This suggests that changes to the morphology and functioning of these cellular organelles, that are used as biomarkers of environmental stress in adult bivalves (i.e. lysosome abundance, alterations to membrane stability, lipofuscin accumulation (Etxeberria et al, 1995, Domouhtsidou and Dimitriadis, 2000, Moore et al, 2007, Rocha et al, 2016) could have similar utility in larvae for evaluating the cytotoxicity of internalized NPs.…”

Section: Discussionmentioning

confidence: 99%

Gold nanoparticles ingested by oyster larvae are internalized by cells through an alimentary endocytic pathway

et al. 2018

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The biological fate of nanoparticles taken up by organisms from their environment is a crucial issue for assessing ecological hazard. Despite its importance, it has scarcely been addressed due to the technical difficulties of doing so in whole organism in vivo studies. Here, by using transmission electron microscopy and energy dispersive X-ray spectroscopy (TEM-EDS), we describe the key aspects that characterize the interaction between an aquatic organism of global ecological and 2 economic importance, the early larval stage of the Japanese oyster (Crassostrea gigas), and model gold nanoparticles dispersed in their environment. The small size of the model organism allowed for a high-throughput visualization of the subcellular distribution of nanoparticles, providing a comprehensive and robust picture of the route of uptake, mechanism of cellular permeation, and the pathways of clearance counterbalancing bioaccumulation. We show that nanoparticles are ingested by larvae and penetrate cells through alimentary pinocytic/phagocytic mechanisms. They undergo intracellular digestion and storage inside residual bodies, before excretion with feces or translocation to phagocytic coelomocytes of the visceral cavity for potential extrusion or further translocation. Our mechanistically-supported findings highlight the potential of oyster larvae and other organisms which feature intracellular digestion processes to be exposed to manmade nanoparticles and thus any risks associated with their inherent toxicity.

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Section: Discussionmentioning

confidence: 99%

Gold nanoparticles ingested by oyster larvae are internalized by cells through an alimentary endocytic pathway

et al. 2018

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“…The responses of digestive gland of mussels to various pollutants exposure of previous results and our present study are in agreement with the following findings of works. Disruption in the epithelial of glands, tissue burden, digestive tube thickness, lumen enlargement, necrotic tissues by heavy metals in Mytilus galloprovincialis [42,43], in mytilus edulis [44], in Perna viridis, Perna indica [45,46], in Crenomytilus grayanus [17]; PAH induced altered diverticula, damages in digestive tubules in Mytilus galloprovincialis [47]; industrial effluents alters vacuolar degeneration in Mytilus galloprovincialis [48]; mixture of micro-plastics altered atrophies, lumen enlargement in Mytilus spp [39]; insecticide damages digestive tubule alterations, hypertrophy, hyperplasia in Mytilus galloprovincialis [36]; heavy metals damages hemocytic infiltration digestive gland, inflammation in Anodonta cygnea [49], condensed nuclei, altered morphology of cell in Dreissena polymorpha [50], increase of atrophic tubule, damages lumen of digestive tubule in Mytilus galloprovincialis [51]; anthropogenic contaminant induces diverticula, inter-tubular tissue necrosis in Ruditapes decussatus [52];…”

Section: Discussionmentioning

confidence: 99%

Histopathology of the Foot, Gill and Digestive Gland Tissues of Freshwater Mussel, Lamellidens marginalis Exposed to Oil Effluent

Balamurugan¹,

Subramanian²

2021

austinjenvirontoxicol

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We investigated the histopathological alterations in the tissues of freshwater mussel, Lamellidens marginalis in response to oil effluent. Based on the previous acute toxicity, two sub lethal [1/4th (11.88ppt) and 1/10th (8.55ppt)] concentrations of oil effluent (hydrocarbon) were prepared and exposed to mussels. In a first series of experiment, animals were exposed/accumulated for 30 days [Ist, 8th, 15th, 22nd and 30th days] by two sub lethal concentrations of oil. In a second series of experiment, oil exposed animals were thereafter transferred to clean water and kept in it up to 30 days [Ist, 8th, 15th, 22nd and 30th days] to assess the recovery pattern (depuration). At seven-day intervals, histopathological alterations were analyzed in foot, gill and digestive gland tissues of mussel. First series of experimental observation showed remarkable damages in foot (disorganized outer epithelium, necrosis of the cell, the formation of lumina, disorganized muscle bundle); in gill (disruption of gill filaments, odema formation, necrosis, dis-aggregated cilia) and in digestive gland (stoma, detached glandular epithelium, vertical clefts, presence of leucocytes, dense accumulation of luminal material) and also oil effluent inducement are confirmed with the aforementioned results. At second series of experiment, it was found that oil effluent tended to accumulate in tissues in a duration-dose-dependent manner. Tissue burden by oil effluent of mussels completely were restored at 30th day. The present experimental findings may be of early warning signals of oil effluent pollution. In conclusion oil effluent are highly toxic to the Lamellidens marginalis.

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“…Vacuolization of the DD was more prevalent in clams from the high p CO 2 treatment after the grow‐out, but the potential mechanisms for this effect are numerous. Although vacuolization is often used as a biomarker of pollutant exposure in bivalves (Neff et al ; Syasina et al ; Usheva et al ; Rocha et al ), it can also be the result of other factors, including disease (Lauckner ), environmental stress (Bright and Ellis ), and surplus lipid storage (Lowe and Clarke ) that may be the indirect result of contaminants, seasonal factors such as phytoplankton abundance or energy storage prior to gametogenesis (Pennec et al ), or calcium ion storage (Taieb ). FTIR responses after the upweller phase are similarly difficult to interpret, especially given that the medium p CO 2 treatment appeared to result in a lower aragonite : calcite ratio than both the ambient and high p CO 2 treatments.…”

Section: Discussionmentioning

confidence: 99%

In situ recovery of bivalve shell characteristics after temporary exposure to elevated pCO₂

Grear

Pimenta

Booth

et al. 2020

Limnology & Oceanography

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Ocean uptake of carbon dioxide (CO 2) is causing changes in carbonate chemistry that affects calcification in marine organisms. In coastal areas, this CO 2-enriched seawater mixes with waters affected by seasonal degradation of organic material loaded externally from watersheds or produced as a response to nutrient enrichment. As a result, coastal bivalves often experience strong seasonal changes in carbonate chemistry. In some cases, these changes may resemble those experienced by aquacultured bivalves during translocation activities. We mimicked these changes by exposing juvenile hard clams (500 μm, Mercenaria mercenaria) to pCO 2 in laboratory upwellers at levels resembling those already reported for northeastern U.S. estuaries (mean upweller pCO 2 = 773, 1274, and 1838 μatm) and then transplanting to three grow-out sites along an expected nutrient gradient in Narragansett Bay, Rhode Island (154 bags of 100 clams). Prior to the field grow-out, clams exposed to elevated pCO 2 exhibited larger shells but lower dry weight per unit volume (dw/V). However, percent increase in dw/V was highest for this group during the 27-day field grow-out, suggesting that individuals with low dw/V after the laboratory treatment accelerated accumulation of dw/V when they were transferred to the bay. Treatments also appeared to affect shell mineral structure and condition of digestive diverticula. Although treatment effects diminished during the field grow-out, clams that were preexposed for several weeks to high pCO 2 would likely have been temporarily vulnerable to predation or other factors that interact with shell integrity. This would be expected to reduce population recovery from short-term exposures to high pCO 2 .

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Histopathological assessment and inflammatory response in the digestive gland of marine mussel Mytilus galloprovincialis exposed to cadmium-based quantum dots

Cited by 59 publications

References 44 publications

Gold nanoparticles ingested by oyster larvae are internalized by cells through an alimentary endocytic pathway

Gold nanoparticles ingested by oyster larvae are internalized by cells through an alimentary endocytic pathway

Histopathology of the Foot, Gill and Digestive Gland Tissues of Freshwater Mussel, Lamellidens marginalis Exposed to Oil Effluent

In situ recovery of bivalve shell characteristics after temporary exposure to elevated pCO₂

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Histopathological assessment and inflammatory response in the digestive gland of marine mussel Mytilus galloprovincialis exposed to cadmium-based quantum dots

Cited by 59 publications

References 44 publications

Gold nanoparticles ingested by oyster larvae are internalized by cells through an alimentary endocytic pathway

Gold nanoparticles ingested by oyster larvae are internalized by cells through an alimentary endocytic pathway

Histopathology of the Foot, Gill and Digestive Gland Tissues of Freshwater Mussel, Lamellidens marginalis Exposed to Oil Effluent

In situ recovery of bivalve shell characteristics after temporary exposure to elevated pCO2

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In situ recovery of bivalve shell characteristics after temporary exposure to elevated pCO₂