Histopathological and Immunohistochemical Findings in Congenital Pouch Colon: A Prospective Study

Udawat, Hema; Nunia, Vandana; Mathur, Praveen; Udawat, Harsh; Gaur, Kusum Lata; Saxena, Amulya K.; Mohan, Monisha

doi:10.1159/000460821

Cited by 11 publications

(11 citation statements)

References 14 publications

(34 reference statements)

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“…However, chemotherapy is often indiscriminate, with many off-targeted toxic effects and is typically only used to treat colon cancers in the early stages of the disease. During this period, cancer cells often acquire drug resistance due to increased exposure to stressful conditions and cytotoxic drugs ( 2 ). This resistance is an important obstacle to treatment that can result in chemotherapy failure in many cancers, including colon cancer ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

Suppression of YAP by DDP disrupts colon tumor progression

Guo

et al. 2018

Oncol Rep

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Colon cancer is a commonly diagnosed cancer that often has a poor prognosis. Combined with the development of drug resistance to cancer treatment agents the treatment efficacy of colon cancer can be limited. Activation of the oncogene YAP has been shown to be related to colon cancer progression and is associated with poor prognosis, drug resistance and metastasis, even under treatment. Cisplatin (DDP) is a commonly used drug that can control carcinoma progression, although its mechanisms are poorly understood. In the present study, we examined whether DDP specifically suppressed YAP in order to inhibit colon carcinoma progression. Our data revealed that Mst/Yap signaling was unusually activated in colon cancers, promoting cell proliferation and invasion. DDP treatment decreased the expression of YAP at both the transcriptional and post-translational levels, leading to cell cycle arrest, apoptosis and senescence in cancer cells, in addition to decreasing epithelial-to-mesenchymal transition, cell motility and in vitro cell invasion and migration. Ultimately, DDP increased the expression of E-cadherin and decreased the expression of vimentin. The present study also revealed that post-translational regulation of YAP phosphorylation controlled the subcellular distribution between the nucleus and the cytoplasm. In conclusion, the findings of the present study revealed that DDP was a suitable therapeutic candidate for colon cancer that specifically targets the Mst/Yap signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Suppression of YAP by DDP disrupts colon tumor progression

Guo

et al. 2018

Oncol Rep

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“…In addition, as mentioned earlier, from our recent histological findings, we revealed the distortion of submucosal and muscularis external linings in diseased condition as compared to normal colon tissue [7]. Although the preliminary reports complementing histopathological studies followed by comparative proteomic analysis indicate a remarkable difference in the expression of few muscular protein coding genes between the normal and affected tissue colon and poorly formed keratin in affected tissues, we argue that there could be enormous effect of poorly formed keratin filaments in the pouch colon patients leading to disrupted muscle fibres (Table 1) as compared to normal colon.…”

Section: Discussionsupporting

confidence: 81%

“…The excised pouch is an abnormal tissue suggested by histopathological examination and immunohistochemistry. There are instances of pouch redilatation in CPC type 1 and 2 in which Coloplasty/ Colography is performed as definitive repair at a later stage which could be due to additional muscle coat seen in CPC type 1 and 2 [7]. Furthermore, pouch excision is rendered by shortening of bowel in most of the cases [8].…”

Section: Introductionmentioning

confidence: 99%

Is Pouch Specific to Colon and Not Ileum?

Gupta

Tiwari

Gupta³

et al. 2019

CPR

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Background: Congenital Pouch Colon (CPC) is an anorectal anomaly with an incidence of 3.5:1 in males and females, respectively. We have earlier reported CPC to be quite prevalent in north Indian tertiary care centers. Objective: n this article, we deliberate on the possible causes associated with CPC bringing the manifestation of the disease. In addition, we throw insights on the effective role of this congenital anomaly in Colon and provide systems genomic evaluation by comparing our recent analysis to that of Colon and Ileum based on Next-Generation Sequencing (NGS) studies. Conclusions: In this commentary article, we argue that a host of epigenetic factors could be the reason why the disease is manifested in colon alone. We further hypothesize on the few unmet challenges linking epigenetics to understand the genetic variants.

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“…In this deformity, varying lengths of the colon are replaced by a pouch like structure that communicates distally with genitourinary tract. Our histopathological and immuno-histochemical findings suggest that CPC has distinct defects in the neuromusculature [1]. An unusual feature of CPC is the prevalence of this malformation in the Indian subcontinent, the highest incidence in the west and north of the India [2].…”

Section: Introductionmentioning

confidence: 59%

Congenital Pouch Colon: Role of Genetics or Environmental Influence?

Mathur¹,

Nunia²,

Sharma³

et al. 2018

Pathobiology

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Background: Congenital pouch colon (CPC), a high type of anorectal malformation, is a sporadic disease and several environmental factors are known to be involved in its pathology. To the best of our knowledge, no familial incidence of CPC has been reported anywhere in the literature so far. Aim: In the present study, which is first of its kind, we have reported the familial incidences of CPC and also tried to elucidate the role of genetics in this pathology. Methods: We have reported 1 familial pedigree of CPC and 2 incidences of dizygotic twins (DZ), out of them one is affected and another one is normal. Highly comprehensive microarray CytoScan HD from Affymetrix was employed to understand the defects underlying submicroscopic genomic imbalance like segment duplication and deletion of the twin patients vis-à-vis their parents and unaffected siblings in these DZ twins. Results: A total of 21 copy number variations (CNVs) were reported in the patient samples that did not overlap with the CNVs in normal parents and healthy sibling, including 5 loss, 3 LOH and 13 gain with size varied from 95 bp to 77 kbp. Genetic analysis revealed involvement of 12 potential genetic loci on Chr 1, 2, 3, 4, 6, 11, and 16. Conclusion: Genetic study found that CPC could be a developmental disorder. These findings are important for further elucidating genetic causes of CPC pathogenesis.

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Histopathological and Immunohistochemical Findings in Congenital Pouch Colon: A Prospective Study

Cited by 11 publications

References 14 publications

Suppression of YAP by DDP disrupts colon tumor progression

Suppression of YAP by DDP disrupts colon tumor progression

Is Pouch Specific to Colon and Not Ileum?

Congenital Pouch Colon: Role of Genetics or Environmental Influence?

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