Histopathologic Limbus Evolution After Alkaline Burns

Abstract

In patients with moderate alkaline burns, AMT improved both limbal stromal and epithelial regeneration more effectively than medical therapy. In patients with severe burns, the best reepithelialization and stromal regeneration were obtained with ALT + AMT.

Temporary Sutureless Amniotic Membrane Patch for Acute Alkaline Burns

“…Encouraging results were noted by some,9,16–18,21,23 but not by others 18,19,24. We speculate that the inconsistency might be due to 2 factors: the severity of the injury, and the rapidity of delivering AMP.…”

“…Consistent with previous studies using conventional AMP with sutures,9,16–18,21 our study demonstrated the effectiveness of the sutureless approach in 5 eyes with grades I to III alkaline burns. In grade IV acute burn, although the use of AMP has been shown to reduce limbal stromal inflammation,23 it is not sufficient to prevent LSCD, presumably because of severe limbal ischemia 9,18,19,24. To combat limbal ischemia in this grade of acute burn, additional procedures such as tenonplasty are required to prevent corneal and scleral melt 7,25,26.…”

Xenotransplantation Using Lyophilized Acellular Porcine Cornea with Cells Grown in vivo and Stimulated with Substance-P

“…With respect to the use of porcine corneas, the most significant limitation is hyperacute or acute rejection, mainly due to xenogenic antigen (Li et al, 1992;Tseng et al, 2005). Several decellularization methods have been reported to decrease the antigenicity by removing stromal cells, but the ideal method for graft survival has not been determined www.intechopen.com Xenotransplantation Using Lyophilized Acellular Porcine Cornea with Cells Grown in vivo and Stimulated with Substance-P 397 (Gilbert et al, 2006;Grauss et al, 2005;López-García et al, 2007). In the current study, we used physical methods to decellularize the porcine cornea by a freeze-thaw technique in combination with a centrifuge.…”

Role of Amniotic Membrane Transplantation in Acute Chemical Injury

“…One patient had grade III injury, whereas the other 4 patients were classified as grade II. 58 Kheirkhah et al 59 retrospectively reported their results with a sutureless approach to temporary delivery of amniotic membrane to the ocular surface using Prokera (Bio-Tissue Inc., Miami, FL), a cryopreserved amniotic membrane clipped into a dual ring system, in a small, uncontrolled, retrospective series of 5 patients with grade I to III acute alkali burns. Furthermore, the patients were found to have stable ocular surface with no cicatricial sequelae in long-term follow-up (mean, 19.6 mo).…”