Histone variants H3.3 and H2A.Z are incorporated into the β-globin locus during transcription activation via different mechanisms

Kang, Jin Gu; Kim, Yea Woon; Kim, AeRi

doi:10.1016/j.bbagrm.2018.05.005

Cited by 9 publications

(9 citation statements)

References 48 publications

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“…A previous study has also shown that H3.3 co-localizes with HIRA complex at the poised enhancers, the regions enriched of H3K4me1 but lack of H3K27ac and p300 ( 53 ). In addition, another recent study has indicated that H2A.Z can co-localize with H3.3 and dynamically incorporate into enhancers during transcriptional activation ( 74 ). Most recently, Zhu and colleagues found that mutation of lysine 27 in histone variant H3.3 results in a dramatic decrease of H3K27ac at enhancers, indicating that enhancers are mainly occupied by H3.3 instead of canonical H3.1/H3.2 ( 75 ).…”

Section: Discussionmentioning

confidence: 99%

HIRA complex presets transcriptional potential through coordinating depositions of the histone variants H3.3 and H2A.Z on the poised genes in mESCs

Yang

Zhang

Xiong

et al. 2021

Nucleic Acids Research

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Histone variants have been implicated in regulating chromatin dynamics and genome functions. Previously, we have shown that histone variant H3.3 actively marks enhancers and cooperates with H2A.Z at promoters to prime the genes into a poised state in mouse embryonic stem cells (mESCs). However, how these two important histone variants collaboratively function in this process still remains elusive. In this study, we found that depletion of different components of HIRA complex, a specific chaperone of H3.3, results in significant decreases of H2A.Z enrichment at genome scale. In addition, CUT&Tag data revealed a genomic colocalization between HIRA complex and SRCAP complex. In vivo and in vitro biochemical assays verified that HIRA complex could interact with SRCAP complex through the Hira subunit. Furthermore, our chromatin accessibility and transcription analyses demonstrated that HIRA complex contributed to preset a defined chromatin feature around TSS region for poising gene transcription. In summary, our results unveiled that while regulating the H3.3 incorporation in the regulatory regions, HIRA complex also collaborates with SRCAP to deposit H2A.Z onto the promoters, which cooperatively determines the transcriptional potential of the poised genes in mESCs.

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Section: Discussionmentioning

confidence: 99%

HIRA complex presets transcriptional potential through coordinating depositions of the histone variants H3.3 and H2A.Z on the poised genes in mESCs

Yang

Zhang

Xiong

et al. 2021

Nucleic Acids Research

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“…Total RNA was extracted from 2 × 10 6 cells using QIAzol Lysis Reagent (Qiagen) and cDNA was generated from 0.5 µg of RNA with random hexamers using the GoScript kit (Promega) as previously described. 35 Quantitative PCR was performed with TaqMan probes in 10 µL of reaction volume using a 7300 Real-time PCR system (Applied Biosystems). The sequences of the probes and primers are listed in Table S3.…”

Section: F I G U R Ementioning

confidence: 99%

Histone H3K4me1 and H3K27ac play roles in nucleosome eviction and eRNA transcription, respectively, at enhancers

et al. 2021

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Enhancers are cis-regulatory elements that highly induce the transcription of target genes. 1,2 They have unique histone modifications, H3K4me1 and H3K27ac, in the chromatin environment. H3K4me1 and H3K27ac are catalyzed by histone methyltransferases MLL3 and MLL4 3,4 and histone acetyltransferases CBP and p300, 5,6 respectively, in mammalian cells. Depletion or inactivation of these histone-modifying enzymes disturbs cell-type-specific gene expression in addition to the severe decrease of H3K4me1 or H3K27ac at enhancers. 7-10 However, the reciprocal effects of these histone modifications on each other at enhancers are not clear. Most studies have focused on one of the histone modifications by targeting histone-modifying enzymes, and controversial results have been obtained from different experimental

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“…Incorporating of histone H2A.Z into chromatin might have allowed enhancers more access by transcription factors or coactivators before transcription initiation. H2A.Z was highly enriched on active enhancers or locus control regions (LCR HSs; HS4, HS2, HS1) of the β-globin locus, either the active γ-globin promoter of the erythroleukemic cell line, K562, or the active β-globin promoter of murine erythroleukemia cells 30 , suggesting its role in chromatin reorganization during erythropoiesis and hemoglobin production. H2A.Z incorporation facilities RNA pol II elongation 31 .…”

Section: Discussionmentioning

confidence: 99%

Down-regulation of the transcriptional repressor ZNF802 (JAZF1) reactivates fetal hemoglobin in β0-thalassemia/HbE

Wongborisuth

Chumchuen

Sripichai

et al. 2022

Sci Rep

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Reactivating of fetal hemoglobin (HbF; α2γ2) can ameliorate the severity of β-thalassemia disease by compensating for adult hemoglobin deficiency in patients. Previously, microarray analysis revealed that zinc finger protein (ZNF)802 (also known as Juxta-posed with another zinc finger gene-1 (JAZF1)) was upregulated in human erythroblasts derived from adult peripheral blood compared with fetal liver-derived cells, implying a potential role as a HbF repressor. However, deficiency in ZNF802 induced by lentiviral shRNA in β0-thalassemia/hemoglobinE erythroblasts had no effect on erythroblast proliferation and differentiation. Remarkably, the induction of HBG expression was observed at the transcriptional and translational levels resulting in an increase of HbF to 35.0 ± 3.5%. Interestingly, the embryonic globin transcripts were also upregulated but the translation of embryonic globin was not detected. These results suggest ZNF802 might be a transcriptional repressor of the γ-globin gene in adult erythroid cells.

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Histone variants H3.3 and H2A.Z are incorporated into the β-globin locus during transcription activation via different mechanisms

Cited by 9 publications

References 48 publications

HIRA complex presets transcriptional potential through coordinating depositions of the histone variants H3.3 and H2A.Z on the poised genes in mESCs

HIRA complex presets transcriptional potential through coordinating depositions of the histone variants H3.3 and H2A.Z on the poised genes in mESCs

Histone H3K4me1 and H3K27ac play roles in nucleosome eviction and eRNA transcription, respectively, at enhancers

Down-regulation of the transcriptional repressor ZNF802 (JAZF1) reactivates fetal hemoglobin in β0-thalassemia/HbE

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