Histone lysine demethylases: emerging roles in development, physiology and disease

Shi, Yang

doi:10.1038/nrg2218

Cited by 539 publications

(459 citation statements)

References 61 publications

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“…Histone demethylases can reverse the effects of methyltransferases by removing methyl groups at specific lysine residues on histones (Shi, 2007), but their role in adult stem cells is still relatively unexplored. However, a few key examples highlight potentially critical functions for these molecules in stem cell maintenance during aging and illustrate the dynamic nature of chromatin changes.…”

Section: Histone Demethylases As Regulators Of Reversible Chromatin Smentioning

confidence: 99%

Epigenetic regulation of aging stem cells

2011

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Section: Histone Demethylases As Regulators Of Reversible Chromatin Smentioning

confidence: 99%

Epigenetic regulation of aging stem cells

2011

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“…10 Vitamin C is also an important cofactor in several enzymatic reactions such as histone demethylases and prolylhydroxylases. 11 Recent studies have demonstrated that vitamin C supplementation produces important effects on stem cell generation, proliferation and differentiation. 12 It was reported that the supplementation of vitamin C with an appropriate dose in culture medium could increase the efficiency of induced pluripotent stem cell generation from human somatic cells, 13 enhance the expansion and osteogenic differentiation of bone marrow-derived mesenchymal stem cells.…”

Section: Introductionmentioning

confidence: 99%

Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

Zhang

et al. 2016

Organogenesis

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ABSTRACT. Although adipose-derived stem cells (ADSCs) have demonstrated a promising potential for the applications of cell-based therapy and regenerative medicine, excessive reactive oxygen species (ROS) are harmful to ADSCs cell survival and proliferation. Vitamin C is an important antioxidant, and is often added into culture media as an essential micronutrient. However, its roles on the proliferation of human ADSCs have not been studied. Therefore, in this study, human ADSCs were isolated, and detected by flow cytometry for the analysis of their cell surface antigens. Cell proliferation and cell cycle progression were measured with cell counting kit-8 assay and flow cytometry, respectively. Western blotting was used to detect the expression levels of cyclin E1, p53, p21, and CDK2 proteins. The effect of vitamin C pretreatment on the production of hydrogen peroxide (H 2 O 2 )-mediated ROS in the ADSCs was evaluated by flow cytometry. Our results indicated that vitamin C treatment significantly increased cell proliferation, and changed the cell cycle distribution of ADSCs by decreasing the percentage of G 1 phase, and concurrently increased the percentage of S and G 2 /M phase. Western blot analysis indicated that vitamin C treatment up-regulated the expression levels of cyclin E1 and CDK2, but down-regulated p53 and p21 proteins expression, which contributed to cell proliferation and cell cycle progression. Vitamin C pretreatment significantly reduced the production of H 2 O 2 -induced ROS in the ADSCs. These findings suggest that vitamin C can promote the proliferation and cell cycle progression in the ADSCs possibly through regulation of p53-p21 signal pathway.

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“…Lysine acetylation and methylation are both reversible processes thanks to the activities of histone deacetylases and recently discovered demethylases [1,4]. Both of these enzyme families are implicated in a range of developmental and physiologic pathways as well as in malignancy [1,5,6].…”

Section: Introductionmentioning

confidence: 99%

Chromatin state maps: new technologies, new insights

Mendenhall

Bernstein

2008

Current Opinion in Genetics & Development

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Recent years have seen unprecedented characterization of mammalian chromatin thanks to advances in chromatin assays, antibody development and genomics. Genome-wide maps of chromatin state can now be readily acquired using microarrays or next-generation sequencing technologies. These datasets reveal local and long-range chromatin patterns that offer insight into the locations and functions of underlying regulatory elements and genes. These patterns are dynamic across developmental stages and lineages. Global studies of chromatin in embryonic stem cells have led to intriguing hypotheses regarding Polycomb/trithorax and RNA polymerase roles in 'poising' transcription. Chromatin state maps thus provide a rich resource for understanding chromatin at a 'systems level', and a starting point for mechanistic studies aimed at defining epigenetic controls that underlie development.

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Histone lysine demethylases: emerging roles in development, physiology and disease

Cited by 539 publications

References 61 publications

Epigenetic regulation of aging stem cells

Epigenetic regulation of aging stem cells

Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

Chromatin state maps: new technologies, new insights

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