Epigenetic regulation of aging stem cells

Pollina, Elizabeth A.; Brunet, Anne

doi:10.1038/onc.2011.45

Cited by 109 publications

(94 citation statements)

References 316 publications

(426 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The involvement of epigenetic dysregulation in the development of various tumors, including osteosarcoma, has been reported. Recent studies on the epigenetic reprogramming of cancer cells have revealed the potent effects of embryonic factors on the reversal of metastatic phenotypes [6,34]. These include studies with amphibian oocyte extracts [1] or zebrafish embryo extracts [8].…”

Section: Discussionmentioning

confidence: 99%

“…Examples of epigenetic modification include DNA methylation and histone modification, both of which regulate gene expression but do not alter the genetic code. For somatic stem cells, epigenetic changes in response to environmental stimuli are important to regulate stem cell function and differentiation [28,34]. For tumor cells, the epigenetic silencing of tumor suppressor genes is associated with tumor formation and progression [2,9,14].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chick Embryo Extract Demethylates Tumor Suppressor Genes in Osteosarcoma Cells

Sultankulov

Agarwal

et al. 2014

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

Background Epigenetics is the study of changes in gene expression or cellular phenotype caused by mechanisms other than changes in the underlying DNA sequence. It is widely accepted that cancer has genetic and epigenetic origins. The idea of epigenetic reprogramming of cancer cells by an embryonic microenvironment possesses potential interest from the prospect of both basic science and potential therapeutic strategies. Chick embryo extract (CEE) has been used for the successful expansion of many specific stem cells and has demonstrated the ability to facilitate DNA demethylation.Questions/purposes The current study was conducted to compare the status of DNA methylation in highly metastatic and less metastatic osteosarcoma cells and to investigate whether CEE may affect the epigenetic regulation of tumor suppressor genes and thus change the metastatic phenotypes of highly metastatic osteosarcoma cells. Methods K7M2 murine OS cells were treated with CEE to determine its potential effect on DNA methylation, cell apoptosis, and invasion capacity. Results Our current results suggest that the methylation status of tumor suppressor genes (p16, p53, and E-cadherin) is significantly greater in highly metastatic mouse ostoesarcoma K7M2 cells in comparison with less metastatic mouse osteosarcoma K12 cells. CEE treatment of K7M2 cells caused demethylation of p16, p53, and E-cadherin genes, upregulated their expression, and resulted in the reversion of metastatic phenotypes in highly metastatic osteosarcoma cells. Conclusions CEE may promote the reversion of metastatic phenotypes of osteosarcoma cells and can be a helpful tool to study osteosarcoma tumor reversion by epigenetic reprogramming. Clinical Relevance Demethylation of tumor suppressor genes in osteosarcoma may represent a novel strategy to diminish the metastatic potential of this neoplasm. Further studies, both in vitro and in vivo, are warranted to evaluate the clinical feasibility of this approach as an adjuvant to current therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chick Embryo Extract Demethylates Tumor Suppressor Genes in Osteosarcoma Cells

Sultankulov

Agarwal

et al. 2014

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

show abstract

“…Early studies of cellular senescence showed that somatic cells can divide for a limited number of times [15]. When this limit is reached, the cells reduce replication and remain viable, but they are not capable of re-entering the cells cycle independently of stimuli received from their surrounding environ-ment [16]. The senescence state is defined as a state during which the cells can no longer divide and it is quite distinct from the quiescent state, where cells are temporarily nonproliferative, but can be induced to divide by appropriate stimuli.…”

Section: Introductionmentioning

confidence: 99%

Morphological, molecular and functional differences of adult bone marrow- and adipose-derived stem cells isolated from rats of different ages

Mantovani

Raimondo

Haneef

et al. 2012

Experimental Cell Research

View full text Add to dashboard Cite

“…6,44,54,55 miRNA'lar normal büyüme ve gelişme, yaş-lanma ve kanser olgularında genlerin düzenlenmesi için oldukça önemlidir. 16 Olgun miRNA 18-25 nük-leotid (nt) uzunluğunda olup ilk olarak saç tokası yapısına sahip primer-miRNA (pri-miRNA) molekülünden transkribe edilir ve translasyon sonrası gen susturulmasında rol alır.…”

Section: Kromati̇n Yeni̇den şEki̇llenmesi̇ Ve Yaşlanmaunclassified

Epigenetic Alterations in Aging and Aging-Associated Diseases: Review

Güneş¹,

Bayramov²

2016

Turkiye Klinikleri J Med Sci

View full text Add to dashboard Cite

Epigenetic regulation of aging stem cells

Cited by 109 publications

References 316 publications

Chick Embryo Extract Demethylates Tumor Suppressor Genes in Osteosarcoma Cells

Chick Embryo Extract Demethylates Tumor Suppressor Genes in Osteosarcoma Cells

Morphological, molecular and functional differences of adult bone marrow- and adipose-derived stem cells isolated from rats of different ages

Epigenetic Alterations in Aging and Aging-Associated Diseases: Review

Contact Info

Product

Resources

About