Histone hyperacetylation may improve the preimplantation development and epigenetic status of cloned embryos

Cui, Kuiqing; Li, Zhipeng; Gao, Bangjun; Jiang, Jianrong; Liu, QingYou; Huang, Bingqing; Shi, Dongquan

doi:10.1016/j.repbio.2020.02.005

Cited by 6 publications

(7 citation statements)

References 41 publications

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“…The process of primary culture, drug treatment, and immunofluorescence of donor cells was in accordance with the experimental procedure of previous article [5]. The ear skin tissue of local pig (born for 1-2 weeks) was collected from the live pig gene bank in Nanning, China.…”

Section: Donor Cells' Treatment and Preparationmentioning

confidence: 99%

“…The process of nuclear transfer and parthenogenetic activation was in accordance with the experimental procedure of previous article [5]. The process of nuclear transfer was that oocytes were enucleated by a blind aspiration to remove the first polar body with the surrounding.…”

Section: Somatic Cell Nuclear Transfer and Parthenogenetic Activationmentioning

confidence: 99%

“…Histone H4K5 and H3K9/K14 acetylation are involved in the protamine replacement and the regulation of chromosome structure [3,4]. Increased H3K9 acetylation in donor cells and nuclear transfer embryos enhances the development efficiency and the expression level of pluripotent genes [5]. A large number of histone H3K9 and H4K8 were acetylated in the expression regions of human and mouse pluripotent genes [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…The granular cells injected into MII oocytes maintain high acetylation levels, but histone acetylation is removed rapidly after the nuclear membrane broken [10]. Increasing H3K9 acetylation of the donor is still erased by histone deacetylase in the cytoplasm [5]. Histone H4K8 and H3K9 acetylation of nuclear transfer embryo is maintained by using broad-spectrum deacetylase inhibitors [2,15,16].…”

Section: Introductionmentioning

confidence: 99%

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HDAC6 Is Involved in the Histone Deacetylation of In Vitro Maturation Oocytes and the Reprogramming of Nuclear Transplantation in Pig

Liu

RuYu

et al. 2021

Reprod. Sci.

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It remained unknown whether HDAC6 affected the histone deacetylation of in vitro maturation oocytes and the reprogramming of nuclear transplantation in pig. Our results indicated that HDAC6 specific inhibition did not affect overall HDAC activity and meiosis process, which increased histone H3K9/K14 and H4K8 acetylation of porcine in vitro maturation oocytes and pseudopronucleus embryos. HDAC6 inhibition also significantly enhanced the cleavage and blastocyst of nuclear transfer embryos (0.81 ± 0.12 vs. 0.68 ± 0.12 and 0.46 ± 0.19; 0.73 ± 0.13 vs. 0.63 ± 0.18 and 0.40 ± 0.16, P<0.05). The inhibition of HDAC6 significantly enhanced histone H3K9/K14 and H4K8 acetylation, and upregulated the OCT4 and CDX2 expressions (1.83 ± 0.16 vs. 1.00 ± 0.00 %; 2.07 ± 0.09 vs. 1.00 ± 0.00; P<0.05) in porcine SCNT blastocysts. Interestingly, HDAC6 inhibition significantly increased the pseudo-pronucleus volume during somatic cell reprogramming. Thus, HDAC6 was required for porcine histone deacetylation during the in vitro maturation and pseudo-pronucleus stages. HDAC6 inhibition improved the in vitro development of nuclear transfer embryos. HDAC6 may restrict the reprogramming of somatic nuclear transfer by regulating pseudo-pronucleus expansion. We need further research to confirm this in the future.

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Section: Donor Cells' Treatment and Preparationmentioning

confidence: 99%

Section: Somatic Cell Nuclear Transfer and Parthenogenetic Activationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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HDAC6 Is Involved in the Histone Deacetylation of In Vitro Maturation Oocytes and the Reprogramming of Nuclear Transplantation in Pig

Liu

RuYu

et al. 2021

Reprod. Sci.

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“…Therefore, such efforts must focus on giving not only comprehensive explanations of underlying biological mechanisms, but also detailed identifications of intrinsic molecular factors that affect proliferative activity, genetic stability, cytophysiological longevity, replicative senescence, physiological ageing, and the apoptotic or autophagy-dependent cell death of cultured nuclear donor cells. This will lead to the epigenetic reprogramming of their cell nuclei both in the host cytoplasm of SCNT-derived oocytes and in the blastomeres of the corresponding cloned embryos (Samiec et al, 2013;Gómez and Pope, 2015;Wang et al, 2020b;Tunstall et al, 2018;Wu et al, 2019;Jeong et al, 2020;Sun et al, 2020).…”

Section: Importance Of Somatic Cell Banking and Scnt-based Cloning For Genetic Rescuing And Maintaining The Genetic Diversity Of Polish Nmentioning

confidence: 99%

Ex Situ Conservation and Genetic Rescue of Endangered Polish Cattle and Pig Breeds with the Aid of Modern Reproductive Biotechnology – A Review

Trzcińska

Samiec

2021

Annals of Animal Science

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The development and optimization of reproductive biotechnology – specifically semen cryopreservation, spermatological diagnostics, and intraspecies cloning by somatic cell nuclear transfer (SCNT) – have become essential techniques to conserve the genetic resources and establish genetic reserves of endangered or vanishing native Polish livestock breeds. Moreover, this biotechnology is necessary for perpetuating biological diversity and enhancing genetic variability as well as for restoring and reintroducing breeds into anthropogenic agricultural ecosystems. On the one hand, the purpose of our paper is to interpret recent efforts aimed at the ex situ conservation of native cattle and pig breeds. On the other, it emphasizes the prominent role played by the National Research Institute of Animal Production (NRIAP) in maintaining biodiversity in agricultural environmental niches. Furthermore, our paper provides an overview of the conventional and modern strategies of the banking and cryopreservation of germplasm-carrier biological materials and somatic cell lines, spermatological diagnostics, and semen-based and SCNT-mediated assisted reproductive technologies (ARTs). These are the most reliable and powerful tools for ex situ protection of the genetic resources of endangered breeds of livestock, especially cattle and pigs.

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Metformin improves epigenetic modification involved in oocyte growth and embryo development in polycystic ovary syndrome mice model

Abkenari

Safdarian

Amidi

et al. 2021

Molecular Reproduction Devel

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The possible relationship between dehydroepiandrosterone (DHEA)‐induced polycystic ovary syndrome (PCOS) and epigenetic changes (ECs) leading to the impaired oocyte quality, has not been investigated yet. So, this study aimed to provide an insight into the relationship of the impaired oocyte quality with ECs in a mice DHEA‐induced PCOS model and to further reveal the effect of metformin treatment. For this purpose, 80 female BALB/C mice were randomly divided into four equal groups, named as the control, sham, (DHEA) and DHEA + Metformin groups. The alterations in acetylation of H4K5 and H4K16, and in methylation of DNA (5MeC) and H3K9 were evaluated using immunocytochemical. Moreover, the expression of Hdac1, Hdac2, Dnmt1, and Dnmt3a genes involved in ECs were analyzed using reverse‐transcription polymerase chain reaction. As well, the levels of mitochondrial membrane potential (MMP), oxidative stress (OS), embryo development, ovarian morphology, sexual hormone, ovulatory function, and AMPKα phosphorylation activity were compared in all the studied groups. Metformin attenuated the damages induced by DHEA as indicated by the normalized the estrous cycle, the improved ovarian morphology, the decreased sexual hormone and OS levels, and the increased MMP and AMPKα phosphorylation levels. In the metformin group, the Dnmt1, Dnmt3a, and Hdac2 genes have significantly upregulated compared to the DHEA group. However, metformin was found to have no effect on the expression level of Hdac1. In this regard, significant decrease and increase were observed in both the acetylated H4K16 and methylated H3K9 within MII oocytes in the DHEA + Metformin group compared with the DHEA group. Our results show that metformin could enhance the developmental competence of PCOS oocytes via reducing OS and ECs.

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Histone hyperacetylation may improve the preimplantation development and epigenetic status of cloned embryos

Cited by 6 publications

References 41 publications

HDAC6 Is Involved in the Histone Deacetylation of In Vitro Maturation Oocytes and the Reprogramming of Nuclear Transplantation in Pig

HDAC6 Is Involved in the Histone Deacetylation of In Vitro Maturation Oocytes and the Reprogramming of Nuclear Transplantation in Pig

Ex Situ Conservation and Genetic Rescue of Endangered Polish Cattle and Pig Breeds with the Aid of Modern Reproductive Biotechnology – A Review

Metformin improves epigenetic modification involved in oocyte growth and embryo development in polycystic ovary syndrome mice model

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