HDAC6 Is Involved in the Histone Deacetylation of In Vitro Maturation Oocytes and the Reprogramming of Nuclear Transplantation in Pig

Abstract

It remained unknown whether HDAC6 affected the histone deacetylation of in vitro maturation oocytes and the reprogramming of nuclear transplantation in pig. Our results indicated that HDAC6 specific inhibition did not affect overall HDAC activity and meiosis process, which increased histone H3K9/K14 and H4K8 acetylation of porcine in vitro maturation oocytes and pseudopronucleus embryos. HDAC6 inhibition also significantly enhanced the cleavage and blastocyst of nuclear transfer embryos (0.81 ± 0.12 vs. 0.68 ± 0.12 and 0.46 ± 0.19; 0.73 ± 0.13 vs. 0.63 ± 0.18 and 0.40 ± 0.16, P<0.05). The inhibition of HDAC6 significantly enhanced histone H3K9/K14 and H4K8 acetylation, and upregulated the OCT4 and CDX2 expressions (1.83 ± 0.16 vs. 1.00 ± 0.00 %; 2.07 ± 0.09 vs. 1.00 ± 0.00; P<0.05) in porcine SCNT blastocysts. Interestingly, HDAC6 inhibition significantly increased the pseudo-pronucleus volume during somatic cell reprogramming. Thus, HDAC6 was required for porcine histone deacetylation during the in vitro maturation and pseudo-pronucleus stages. HDAC6 inhibition improved the in vitro development of nuclear transfer embryos. HDAC6 may restrict the reprogramming of somatic nuclear transfer by regulating pseudo-pronucleus expansion. We need further research to confirm this in the future.

LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6

“…To our knowledge, HDAC6 could deacetylate H3K9 (Ortega et al, 2021;Sun et al, 2021), and deacetylation of H3K9 at the promoter usually leads to reduced transcription (Chen et al, 2021;Kawamura et al, 2021). Therefore, ChIP assay followed by PCR and qPCR was performed by H3K9ac antibody to identify the effect of lncRNA NORAD on H3K9 deacetylation at the VEGF gene promoter in ox-LDL-treated HUVECs.…”

Antisense oligonucleotide-mediated perturbation of long non-coding RNA reveals functional features in stem cells and across cell types

“…1 HDAC6 translates to histone deacetylase and suppresses multiple genes during differentiation. 39 Inhibition of it up-regulated OCT4 in porcine blastocysts 40 while HDAC inhibitor was shown to improve iPS cell reprogramming from peripheral blood mononuclear cells. 41…”

Insufficient HtrA2 causes meiotic defects by inducing cytoskeletal disorganization in aging germinal vesicle oocytes

“…Based on these ndings, we investigated how HtrA2 modulated α-tubulin acetylation. Several deacetylases have been reported to participate in α-tubulin acetylation in somatic cells and oocytes [24,35,[48][49][50]. Histone deacetylase inhibitors were found to boost BAX levels in pancreatic adenocarcinoma cell lines, as well as promote the release of apoptosis-inducing factor and Omi/HtrA2 from the mitochondria, thereby inducing apoptosis [51].…”