Histone H1—DNA interaction. On the mechanism of DNA strands crosslinking by histone H1

“…Studies on the relationship of nucleosomal repeat sizes and altered amounts of Hi and H5 in erythroid cells failed to show a correlation (53). This was unexpected, since Hi and H5 both bind to linker regions and vary in charge and size (54).…”

Section: Discussionmentioning

confidence: 97%

Organizational changes in chromatin at different malignant stages of Friend erythroleukemia

Leonardson¹,

Levy²

1981

Nucl Acids Res

View full text Add to dashboard Cite

The chromatin structure of morphologically-similar, but increasinglymalignant erythroleukemia cells was investigated using mild micrococcal nuclease digestion of isolated nuclei. The maximum solubilization of chromatin was unique for each of the three cell types: the least malignant (our (190 bp). In addition, while the DNA of mononucleosomes depleted in Hi was smaller than that in the Hicontaining species, relative size differences among the different cell types were retained. These data suggested that the difference in the mononucleosome particle size resistant to nuclease digestion was independent of histone Hi. Differences in nucleosome repeat length were also noted among the cell types. These studies have demonstrated dramatic differences in chromatin structure associated with malignant potential of an otherwise morphologically identical cell type. These findings may reflect changes in the relative amounts of H2a variants which we have previously described among the different malignant cell types.

show abstract

“…This procedure is adequate for the study of the interrelationship between complex size and transfection. With further increasing salt concentration, binding interactions between H1 and DNA decrease, which results, after the disintegration of the aggregates, in the expansion of the resulting complexes and dissociation at about 0.6 M NaCl [3,5]. This process is accompanied by a decreasing size of the complexes.…”

Section: Discussionmentioning

confidence: 99%

“…These observations have been made for both lipid-and polycation-mediated gene transfer. Previously, chromatin research faced similar problems with solubility [1,2], and this holds true also for H1 (histone H1)-DNA complexes [3,4]. In a previous paper we reported that aggregates of H1-DNA complexes formed in 0.15 M NaCl were the most transfection-efficient part of a heterogeneous H1-DNA complex population.…”

Section: Introductionmentioning

confidence: 86%

“…In order to understand why these small compact complexes were inactive, we evaluated in the present study the transfection/uptake of H1–DNA complexes of various sizes. The different complex sizes were generated by using salt concentrations known to determine the complex sizes [3,6]. This procedure was necessary because the fractionation of H1–DNA complexes by the usual procedures of centrifugation or gel filtration in physiological salt resulted in aggregating complexes again being formed, a phenomenon that excluded clear separation results.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structural aspects of histone H1–DNA complexes and their relation to transfection efficiency

Haberland

Cartier

Heuer

et al. 2005

Biotech and App Biochem

View full text Add to dashboard Cite

During transfection, polycation-DNA complexes are normally diluted by the transfection medium, which often contains salt in the physiological concentration range and serum. It is not exactly known to what extent this dilution step influences the properties of the complexes, which in turn influence the transfection efficiency. In order to gain more insight into the size-structure-transfection activity relationship, we prepared histone H1-DNA complexes in NaCl solutions at various concentrations known to determine the size and structure of the resulting complexes. We characterized the complexes by physicochemical methods. Fluorescence correlation spectroscopy enabled relative measurements of complex sizes even under physiological conditions. The different appearances of the complexes were correlated with their transfection efficiency. When transfection was performed by dilution of the complexes in cell-cultivation media, the initial structure of H1-DNA complexes preformed under distinct salt conditions had no significant influence on the transfection efficiency. The dilution of the preformed complexes with cell-cultivation medium resulted in re-formation and aggregation of the complexes. The addition of the complexes to the cells without cell-cultivation medium, however, showed a direct correlation between the size of the complexes and the transfection efficiency (correlation coefficient 0.91). Small complexes did not contribute to the transfection.

show abstract

Histone H1—DNA interaction. On the mechanism of DNA strands crosslinking by histone H1

Cited by 21 publications

References 24 publications

Mutual arrangement of histone H1 molecules in chromatin of intact nuclei

Mutual arrangement of histone H1 molecules in chromatin of intact nuclei

Organizational changes in chromatin at different malignant stages of Friend erythroleukemia

Structural aspects of histone H1–DNA complexes and their relation to transfection efficiency

Contact Info

Product

Resources

About