Mutual arrangement of histone H1 molecules in chromatin of intact nuclei

Николаев, Л. Г.; Glotov, Boris O.; Itkes, A.V.; Severin, E. S.

doi:10.1016/0014-5793(81)80987-8

Cited by 17 publications

(12 citation statements)

References 15 publications

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“…The N-terminal half of Hl histone includes the so cahed 'nose' and 'head' of the molecule, while practically only the 'tail' of the Hl is present in the C-terminal half 19, lo] _ Using the chymot~~~ digestion of ~ross~li~k~d Hl histone dimers, it is possible to reveal closelyspaced HI chymotryptic halves belonging to different molecules both in nuclei [7] and in extended chromatin. For such an analysis, digested Hl dimers were resolved in SDS-containing polyacrylamide gel in the first dimension and, after splitting of the cross-links with 2-mer~apt~th~ol, were further electrophoresed in the second dimension on the same gel.…”

Section: Resultsmentioning

confidence: 99%

“…Histone Hl dimers were isolated by gel filtration on a 1 S x 100 cm column of Sephadex G-200 [7], equilibrated with 10 mM HCl.…”

Section: Methodsmentioning

confidence: 99%

“…Earlier we studied the proximity between the Nand C-terminal parts of Hl molecules in compact chromatin of intact nuclei using ch~otr~tic digestion of cross-linked Hl histone dimers [7].…”

Section: Protein Crop-linkingmentioning

confidence: 99%

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Mutual arrangement of histone H1 molecules in extended chromatin

Николаев¹,

Glotov²,

Dashkevich³

et al. 1983

FEBS Letters

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Mutual arrangement of histone H1 molecules in chromatin extended in low salt—EDTA buffer and additionally in the presence of urea was studied by means of reversible cross‐linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross‐linked in all possible combinations; i.e., C—C, C—N and N—N. The results imply that the mutual arrangement of H1 histones is determined by the structure of extended nucleosomal chain, rather than chromatin superstructure.

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Section: Resultsmentioning

confidence: 99%

“…Histone Hl dimers were isolated by gel filtration on a 1 S x 100 cm column of Sephadex G-200 [7], equilibrated with 10 mM HCl.…”

Section: Methodsmentioning

confidence: 99%

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Mutual arrangement of histone H1 molecules in extended chromatin

Николаев¹,

Glotov²,

Dashkevich³

et al. 1983

FEBS Letters

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“…The HCI extracts were prepared for electrophoresis as above. Diagonal gel electrophoresis of the extracted histones and their crosslinked oligomers was done in 0.1 x 15 x 15 cm polyacrylamide slab gels in the presence of Na-dodecylsulphate [20] as in [21]. Prior to the separation in the second dimension the S-S bridges of the crosslinks were split with 2-mercaptoethanol.…”

Section: Methodsmentioning

confidence: 99%

“…Individual histone constituents from separated histone heterooligomers fall on the corresponding vertical lines. [21].…”

Section: Identification Of Histones Crosslinked In Compact Nuclear Chmentioning

confidence: 99%

Chemical crosslinking of histone H1° to histone neighbours in nuclei and chromatin

Dashkevich¹,

Николаев²,

Zlatanova

et al. 1983

FEBS Letters

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The arrangement of H5 molecules in extended and condensed chicken erythrocyte chromatin.

Lennard¹,

Thomas²

1985

The EMBO Journal

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Chemical cross‐linking with dithiobis(succinimidyl propionate) has been used to investigate the relative disposition of neighbouring H5 (H1) molecules in chicken erythrocyte chromatin in the extended (nucleosome filament) and condensed (300 A filament) states; in this chromatin H5 and H1 are interspersed along the nucleosome filament, rather than segregated into blocks, as shown by the nature of the cross‐linked dimers and their relative amounts. Detailed analysis of the cross‐linked H5 homopolymers from extended chromatin and condensed nuclear chromatin indicates which domains of H5 are in contact (or close proximity) in the two states. Two results suggest a polar, head‐to‐tail arrangement of H5 molecules along the nucleosome filament. This arrangement persists when chromatin adopts higher‐order structure but in the folded state neighbouring basic C‐terminal domains, in particular, are more closely juxtaposed than they are in extended chromatin.

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Mutual arrangement of histone H1 molecules in chromatin of intact nuclei

Cited by 17 publications

References 15 publications

Mutual arrangement of histone H1 molecules in extended chromatin

Mutual arrangement of histone H1 molecules in extended chromatin

Chemical crosslinking of histone H1° to histone neighbours in nuclei and chromatin

The arrangement of H5 molecules in extended and condensed chicken erythrocyte chromatin.

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