Histone demethylase KDM5A regulates the functions of human periodontal ligament stem cells during periodontitis via the miR-495-3p/HOXC8 axis

Niu, Fujun; Xu, Jing; Yan, Yongsheng

doi:10.1016/j.reth.2021.12.002

Cited by 3 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, KDM5A regulates osteogenic differentiation by controlling H3K4me3 of SOCS1 32 . Additionally, KDM5A can regulate the demethylation of H3K4me3 on the promoter of miR‐495‐3p to inhibit its expression 38 . Knockdown of KDM5A in human dental pulp cells leads to an increase in H3K4me3 levels on odontogenic marker promoters 14 .…”

Section: Discussionmentioning

confidence: 99%

“…32 Additionally, KDM5A can regulate the demethylation of H3K4me3 on the promoter of miR-495-3p to inhibit its expression. 38 Knockdown of KDM5A in human dental pulp cells leads to an increase in H3K4me3 levels on odontogenic marker promoters. 14 In future studies, we aim to validate the mechanism identified in this study in animal models, explore more upstream regulators of KDM5A, and investigate the downstream mechanism of KDM5A in osteogenic differentiation in SONFH.…”

Section: Mir-107 Inhibited Kdm5a Expression To Promote Runx2 Ocn and ...mentioning

confidence: 99%

See 1 more Smart Citation

Mechanism of KDM5A‐mediated H3K4me3 modification in the osteogenic differentiation of mesenchymal stem cells in steroid‐induced osteonecrosis of the femoral head

Yan,

Qiu

et al. 2024

Int J of Rheum Dis

View full text Add to dashboard Cite

ObjectivesSteroid‐induced osteonecrosis of the femoral head (SONFH) is characterized by impaired osteogenesis in bone marrow mesenchymal stem cells (BMSCs). This study investigates the role of lysine‐specific demethylase 5A (KDM5A) in SONFH to identify potential therapeutic targets.MethodsHuman BMSCs were isolated and characterized for cell surface markers and differentiation capacity. A SONFH cell model was established using dexamethasone treatment. BMSCs were transfected with KDM5A overexpression vectors or si‐KDM5A, and the expression of KDM5A, miR‐107, runt‐related transcription factor 2 (RUNX2), osteocalcin (OCN), and osteopontin (OPN) was assessed. Alizarin red staining was used to observe mineralization nodules, while alkaline phosphatase activity and cell viability were measured. The enrichment of KDM5A and histone 3 lysine 4 trimethylation (H3K4me3) on the promoters of RUNX2, OCN, and OPN was analyzed. The binding between miR‐107 and KDM5A 3′UTR was validated, and the combined effect of miR‐107 overexpression and KDM5A overexpression on BMSC osteogenic differentiation was evaluated.ResultsKDM5A was upregulated in BMSCs from SONFH. Inhibition of KDM5A promoted osteogenic differentiation of BMSCs, associated with increased RUNX2, OCN, and OPN promoters. KDM5A bound to the promoters of RUNX2, OCN, and OPN, leading to reduced H3K4me3 levels and downregulation of their expression. Overexpression of miR‐107 inhibited KDM5A and enhanced BMSC osteogenic differentiation.ConclusionKDM5A negatively regulates BMSC osteogenic differentiation by modulating H3K4me3 levels on the promoters of key osteogenic genes. miR‐107 overexpression counteracts the inhibitory effect of KDM5A on osteogenic differentiation. These findings highlight the potential of targeting the KDM5A/miR‐107 axis for SONFH therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Mir-107 Inhibited Kdm5a Expression To Promote Runx2 Ocn and ...mentioning

confidence: 99%

Mechanism of KDM5A‐mediated H3K4me3 modification in the osteogenic differentiation of mesenchymal stem cells in steroid‐induced osteonecrosis of the femoral head

Yan,

Qiu

et al. 2024

Int J of Rheum Dis

View full text Add to dashboard Cite

show abstract

Bisphenol S induces brown adipose tissue whitening and aggravates diet-induced obesity in an estrogen-dependent manner

Wen,

Xiao,

Xiao

et al. 2023

Cell Reports

View full text Add to dashboard Cite

Omentin-1 attenuates lipopolysaccharide-induced inflammation and osteogenic differentiation in periodontal ligament stem cells and reduces M1 macrophages polarization through repressing endoplasmic reticulum stress

Yang,

Chen

2024

Prostaglandins & Other Lipid Mediators

View full text Add to dashboard Cite

Histone demethylase KDM5A regulates the functions of human periodontal ligament stem cells during periodontitis via the miR-495-3p/HOXC8 axis

Cited by 3 publications

References 38 publications

Mechanism of KDM5A‐mediated H3K4me3 modification in the osteogenic differentiation of mesenchymal stem cells in steroid‐induced osteonecrosis of the femoral head

Mechanism of KDM5A‐mediated H3K4me3 modification in the osteogenic differentiation of mesenchymal stem cells in steroid‐induced osteonecrosis of the femoral head

Bisphenol S induces brown adipose tissue whitening and aggravates diet-induced obesity in an estrogen-dependent manner

Omentin-1 attenuates lipopolysaccharide-induced inflammation and osteogenic differentiation in periodontal ligament stem cells and reduces M1 macrophages polarization through repressing endoplasmic reticulum stress

Contact Info

Product

Resources

About