Histone deacetylase inhibitor stimulates E2 and P4 secretion in sika deer ovarian granulosa cells at a moderate dose

Abstract

The histone deacetylase inhibitor (HDACi) and tumor suppressor play an important role in genome reorganization and epigenetic regulation. In this study, granulosa cells (GCs) isolated from sika deer ovaries were cultured and treated with different concentrations of trichostatin A (TSA) for 48 h. It was found that TSA inhibited GCs proliferation and induced GCs apoptosis by upregulating expression of BAX, meanwhile, downregulating expression of GLUT3, GLUT8, BCL-XL. In addition, TSA caused cell cycle arrest at the G1 and G2/M phase accompanied by reducing expression of Cyclin D2 and CDK4. TSA pretreatment increased DNMT3a, DNMT1, HDAC1, and HAT1 expression, and attenuated them when TAS higher than 50 nM. The protein levels of H3K9ac and H4K8ac in GCs were increased at 48 h after TSA treatment. TSA stimulated the secretion of estradiol and progesterone at a moderate dose. Our data suggest that TSA is important as a regulator of steroid hormone synthesis in granulosa cells during follicular development in the sika deer ovary.

Effects of 5‐Aza‐2'‐deoxycytidine on hormone secretion and epigenetic regulation in sika deer ovarian granulosa cells

“…On day 8, follicle‐stimulating hormone (FSH) was administered by intramuscular injection daily for 4 d. On day 10, the CIDRs were removed, and the hinds were given an intramuscular injection of 0.1 mg cloprostenol (PG, Munitions University Veterinary Institute, China) to stimulate superovulation. On day 12, we collected ovum and GCs from the largest follicles by laparoscopic follicular aspiration (Xing et al., 2018) (Figure 1a). There were remarkable follicles in the superovulation ovaries (Figure 1b).…”

Identification of the JY‐1 in sika deer and its effects on granulosa cells cultured in vitro

“…Total RNA was then isolated using Trizol (Invitrogen Inc.) according to the manufacturer's instructions. Quantitative real‐time PCR using gene‐specific primers for JY‐1 knockdown of RNA was performed per a previous study (Xing et al, ). Controls were NC group.…”

Epigenetic regulation in premature ovarian failure: A literature review

“…According to He et al, specific disruption of Lsd1 resulted in significantly increased autophagy through its H3K4me2 demethylase activity and a decreased oocyte number in perinatal mice, leading to the depletion of oocytes ( He et al, 2020 ). In contrast, some studies confirm that treatment with HDAC inhibitors, such as TSA or butyrate, may promote steroid hormone synthesis in GCs during follicular development by differentially regulating gene expression ( Xing et al, 2018 ; Ye et al, 2021 ).…”