Histone deacetylase III interactions with BK polyomavirus large tumor antigen may affect protein stability

Abstract

Background Human polyomavirus BK (BKPyV) causes associated nephropathy and contributes to urinary tract cancer development in renal transplant recipients. Large tumor antigen (LT) is an early protein essential in the polyomavirus life cycle. Protein acetylation plays a critical role in regulating protein stability, so this study investigated the acetylation of the BKPyV LT protein. Methods The BKPyV LT nucleotide was synthesized, and the protein was expressed by transfection into permissive cells. The BKPyV LT protein was immunoprecipitated and subjected to LC-MS/MS analysis to determine the acetylation residues. The relative lysine was then mutated to arginine in the LT nucleotide and BKPyV genome to analyze the role of LT lysine acetylation in the BKPyV life cycle. Results BKPyV LT acetylation sites were identified at Lys3 and Lys230 by mass spectrometry. HDAC3 and HDAC8 and their deacetylation activity are required for BKPyV LT expression. In addition, mutations of Lys3 and Lys230 to arginine increased LT expression, and the interaction of HDAC3 and LT was confirmed by coimmunoprecipitation. Conclusions HDAC3 is a newly identified protein that interacts with BKPyV LT, and LT acetylation plays a vital role in the BKPyV life cycle.

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“…The immunoprecipitates were separated by SDS-PAGE, and the gel was stained with PageBlue Protein Staining Solution (ThermoFisher, Rockford, IL, USA) to visualize the protein bands. The band of interest was excised from the gel and subjected to in-gel digestion as described previously [ 36 ]. The resulting peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an Orbitrap Fusion mass spectrometer (ThermoFisher) equipped with an EASY-nLC 1200 system (ThermoFisher) and an EASY-Spray HPLC column (75 μm × 150 mm, 3 μm, 100 Å).…”