Histomorphorlogical and biochemical evaluation of oral administration of datura metel stramonium on the kidneys of adult male wistar rats

Abstract

Datura metel stramonium (leaves) extract has traditionally been used to treat swellings, burns, ulcers, asthma and sinus infections. However, report indicate that this plant is a strong poison and has been linked to delirium and acute poisoning, which may result in death. Aim: Histomorphological potentials and biochemical effects of Datura metel stramonium on the kidneys of adult male Wistar rats. Fresh Datura metel leaves were harvested, cleaned, air-dried and crushed into fine powder and cold macerated in 0.5 L of 80% v/v methanol in water for 72 hours and the resultant mixture was filtered using Whatman filter paper (No.1). The filtrate was condensed to dryness in vapour at 400C using water bath, yielding 9g (18% w/w) of a semi-solid extract and stored in the refrigerator at 40C until use. Twenty-four (24) adult male Wistar rats were divided into four (4) groups of six (6) animals each. Groups 1 (0.2ml of distilled water), 2 (200 mg/kg), 3 (400 mg/kg), and 4 (600 mg/kg) body weight/day had oral administration for twenty-eight (28) days. The Wistar rats were sacrificed by cervical dislocation and kidney tissues were excised for histomorphological and biochemical examinations.