Histomorphometry and micro‐computed tomography of bone augmentation under a titanium membrane

Maréchal, Marina; Luyten, Frank P.; Nijs, J; Постнов, А. А.; Schepers, Evert; Steenberghe, Daniël van

doi:10.1111/j.1600-0501.2005.01205.x

Cited by 33 publications

(43 citation statements)

References 11 publications

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“…This result was similar to previous studies. Maréchal et al [19] reported a strong correlation between micro-CT and serial histological sections of bone augmentation under a titanium barrier membrane with r 2 =0.072. Umoh et al [20] compared the histological assessments and in vivo micro-CT images in a rat calvarial model and also observed a high correlation between both examinations with r 2 =0.70.…”

Section: Discussionmentioning

confidence: 99%

The evaluation of the correlation between histomorphometric analysis and micro-computed tomography analysis in AdBMP-2 induced bone regeneration in rat calvarial defects

Park

Kim

Koo

et al. 2011

J Periodontal Implant Sci

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PurposeMicro-computed tomography (micro-CT) has been widely used in the evaluation of regenerated bone tissue but the reliability of micro-CT has not yet been established. This study evaluated the correlation between histomorphometric analysis and micro-CT analysis in performing new bone formation measurement.MethodsCritical-size calvarial defects were created using a 8 mm trephine bur in a total of 24 Sprague-Dawley rats, and collagen gel mixed with autogenous rat bone marrow stromal cells (BMSCs) or autogenous rat BMSCs transduced by adenovirus containing bone morphogenic protein-2 (BMP-2) genes was loaded into the defect site. In the control group, collagen gel alone was loaded into the defect. After 2 and 4 weeks, the animals were euthanized and calvaria containing defects were harvested. Micro-CT analysis and histomorphometric analysis of each sample were accomplished and the statistical evaluation about the correlation between both analyses was performed.ResultsNew bone formation of the BMP-2 group was greater than that of the other groups at 2 and 4 weeks in both histomorphometric analysis and micro-CT analysis (P=0.026, P=0.034). Histomorphometric analysis of representative sections showed similar results to histomorphometric analysis with a mean value of 3 sections. Measurement of new bone formation was highly correlated between histomorphometric analysis and micro-CT analysis, especially at the low lower threshold level at 2 weeks (adjusted r2=0.907, P<0.001). New bone formation of the BMP-2 group analyzed by micro-CT tended to decline sharply with an increasing lower threshold level, and it was statistically significant (P<0.001).ConclusionsBoth histomorphometric analysis and micro-CT analysis were valid methods for measurement of the new bone in rat calvarial defects and the ability to detect the new bone in micro-CT analysis was highly influenced by the threshold level in the BMP-2 group at early stage.

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Section: Discussionmentioning

confidence: 99%

The evaluation of the correlation between histomorphometric analysis and micro-computed tomography analysis in AdBMP-2 induced bone regeneration in rat calvarial defects

Park

Kim

Koo

et al. 2011

J Periodontal Implant Sci

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“…Direct comparison of micro-CT images with histology in rat confirmed good agreement with respect to fine bone detail. Marechal et al (16) used micro-CT and histological sections to examine the extent of bone augmentation underneath an occlusive membrane; the results were highly correlated. Umoh et al (17) compared histological assessments and in vivo micro-CT images and observed consistent patterns of bone formation in both.…”

Section: Discussionmentioning

confidence: 99%

A comparative study of microfocus CT and histomorphometry in the evaluation of bone augmentation in rat calvarium

et al. 2010

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Microfocus computed tomography (micro-CT; R_mCT) is a dynamic noninvasive method for measuring bone regeneration. This study evaluated whether R_mCT was equivalent to histomorphometry in assessing bone augmentation. Two plastic caps of graft material with (experiment) or without hydroxyapatite (HA; control) were placed in the exposed calvaria of rats. Images of bone augmentation within the plastic caps were then taken using R_mCT. Histological sections were cut along the same plane as that used for the micro-CT images. Bone regeneration beyond the skeletal envelope occurred at both the experimental and control sites. Bone volume also increased at both sites. In addition, consistent patterns of bone formation were observed in both R_mCT and histological images. R_mCT analysis enables highly quantitative and qualitative measurement of bone augmentation in living animals. (J Oral Sci 52, 203-211, 2010)

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“…However, when imaging the ECM or tissue growth in metallic scaffolds or around implants, additional caution has to be taken during image analysis as metal artifacts 20,21 can significantly influence the accuracy of the quantification of the newly formed ECM or tissue volume. 22,23 Several studies have shown that when using phase-contrast imaging, in most cases only available by synchrotron radiation, the nonmineralized ECM formed in vitro in 3D TE constructs can be visualized. [24][25][26] However, routine access to systems allowing phase-contrast imaging is limited and there are restrictions on the sample specifications.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

Papantoniou

Sonnaert

Geris

et al. 2014

Tissue Engineering Part C: Methods

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To successfully implement tissue-engineered (TE) constructs as part of a clinical therapy, it is necessary to develop quality control tools that will ensure accurate and consistent TE construct release specifications. Hence, advanced methods to monitor TE construct properties need to be further developed. In this study, we showed proof of concept for contrast-enhanced nanofocus computed tomography (CE-nano-CT) as a whole-construct imaging technique with a noninvasive potential that enables three-dimensional (3D) visualization and quantification of in vitro engineered extracellular matrix (ECM) in TE constructs. In particular, we performed a 3D qualitative and quantitative structural and spatial assessment of the in vitro engineered ECM, formed during static and perfusion bioreactor cell culture in 3D TE scaffolds, using two contrast agents, namely, Hexabrix Ò and phosphotungstic acid (PTA). To evaluate the potential of CE-nano-CT, a comparison was made to standardly used techniques such as Live/Dead viability/cytotoxicity, Picrosirius Red staining, and to net dry weight measurements of the TE constructs. When using Hexabrix as the contrast agent, the ECM volume fitted linearly with the net dry ECM weight independent from the flow rate used, thus suggesting that it stains most of the ECM. When using PTA as the contrast agent, comparing to net weight measurements showed that PTA only stains a part of the ECM. This was attributed to the binding specificity of this contrast agent. In addition, the PTA-stained CE-nano-CT data showed pronounced distinction between flow conditions when compared to Hexabrix, indicating culture-specific structural ECM differences. This novel type of information can contribute to optimize bioreactor culture conditions and potentially critical quality characteristics of TE constructs such as ECM quantity and homogeneity, facilitating the gradual transformation of TE constructs in well-characterized TE products.

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Histomorphometry and micro‐computed tomography of bone augmentation under a titanium membrane

Cited by 33 publications

References 11 publications

The evaluation of the correlation between histomorphometric analysis and micro-computed tomography analysis in AdBMP-2 induced bone regeneration in rat calvarial defects

The evaluation of the correlation between histomorphometric analysis and micro-computed tomography analysis in AdBMP-2 induced bone regeneration in rat calvarial defects

A comparative study of microfocus CT and histomorphometry in the evaluation of bone augmentation in rat calvarium

Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

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