A comparative study of microfocus CT and histomorphometry in the evaluation of bone augmentation in rat calvarium

Kochi, Go; Sato, Shuichi; Ebihara, Hajime; Hirano, Jiro; Arai, Yoshinori; Ito, Koichi

doi:10.2334/josnusd.52.203

Cited by 29 publications

(35 citation statements)

References 17 publications

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“…The design of the skeletal envelope in the calvarium was according to the surgical procedure reported by us previously 1) . The advantages of our model include a lack of spontaneous regeneration and a standardized defect size by the use uniform plastic caps.…”

Section: Discussionmentioning

confidence: 99%

“…Although we have investigated the effect of various regenerative factors and biomaterials on GBA in rat calvarium, it was difficult to accurately measure the significant amount and height of newly generated bone in every instance [1][2][3] . Platelet-derived growth factor (PDGF) is a potent mitogen and chemotactic factor for mesenchymal cells.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Platelet-Derived Growth Factor on Enhanced Bone Augmentation beyond the Skeletal Envelope within a Plastic Cap in the Rat Calvarium

Tsuchiya

Sato

Kigami

et al. 2013

J. Hard Tissue Biology.

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We evaluated the ability of platelet-derived growth factor (PDGF) to promote bone augmentation beyond the skeletal envelope in rat calvarium. The calvaria of 14 rats were exposed and two plastic caps were placed with 0.03% or 0.01% PDGF with a collagen sponge (ACS) or ACS alone in the calvarium. Micro-CT and histological sections were used to obtain amount of bone augmentation within the plastic caps. Bone volume (BV) was calculated using BV-measuring software. The BV and amount of bone augmentation were significantly increased in the PDGF groups compared to the control groups after 4 weeks. However, there was no significant difference between the 0.03% and 0.01% PDGF groups. These results indicate that 0.03% and 0.01% PDGF in ACS enhance bone formation beyond the skeletal envelope in rat calvarium.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Platelet-Derived Growth Factor on Enhanced Bone Augmentation beyond the Skeletal Envelope within a Plastic Cap in the Rat Calvarium

Tsuchiya

Sato

Kigami

et al. 2013

J. Hard Tissue Biology.

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“…In the AB group, a rat GBA model reported and standardized previously by our group was applied by setting plastic caps on the rat calvaria [12,13]. All operations were conducted under sterile conditions.…”

Section: Primary Surgerymentioning

confidence: 99%

“…To overcome these limits, procedures using a bone substitute [12,13] or bioactive molecules [14,15] are used with the original GBR theory. Even if adequate BV is acquired successfully by the GBR procedure, a bony ridge in the desired shape is rarely obtained.…”

Section: Introductionmentioning

confidence: 99%

Regenerative capacity of augmented bone in rat calvarial guided bone augmentation model

Kubota

Hasuike

Ozawa

et al. 2017

J Periodontal Implant Sci

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Purpose: Guided bone regeneration (GBR) is the most widely used technique to regenerate and augment bones. Even though augmented bones (ABs) have been examined histologically in many studies, few studies have been conducted to examine the biological potential of these bones and the healing dynamics following their use. Moreover, whether the bone obtained from the GBR procedure possesses the same functions as the existing autogenous bone is uncertain. In particular, little attention has been paid to the regenerative ability of GBR bone. Therefore, the present study histologically evaluated the regenerative capacity of AB in the occlusive space of a rat guided bone augmentation (GBA) model. Methods: The calvaria of 30 rats were exposed, and plastic caps were placed on the right of the calvaria in 10 of the 30 rats. After a 12-week healing phase, critical-sized calvarial bone defects (diameter: 5.0 mm) were trephined into the dorsal parietal bone on the left of the calvaria. Bone particles were harvested from the AB or the cortical bone (CB) using a bone scraper and transplanted into the critical defects. Results: The newly generated bone at the defects' edge was evaluated using micro-computed tomography (micro-CT) and histological sections. In the micro-CT analysis, the radiopacity in both the augmented and the CB groups remained high throughout the observational period. In the histological analysis, the closure rate of the CB was significantly higher than in the AB group. The numbers of cells positive for runt-related transcription factor 2 (Runx2) and tartrateresistant acid phosphatase (TRAP) in the AB group were larger than in the CB group. Conclusions: The regenerative capacity of AB in the occlusive space of the rat GBA model was confirmed. Within the limitations of this study, the regenerative ability of the AB particulate transplant was inferior to that of the CB particulate transplant.

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“…We previously studied the effects of various regenerative factors and biomaterials on GBA in rat calvaria but found it difficult to accurately measure the amount and height of newly generated bone in all specimens (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

Effect of a chitosan sponge impregnated with platelet-derived growth factor on bone augmentation beyond the skeletal envelope in rat calvaria

et al. 2014

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Abstract:We evaluated the ability of platelet-derived growth factor (PDGF) to promote bone augmentation beyond the skeletal envelope in rat calvaria. The calvariae of 14 rats were exposed, and two plastic caps-one with 0.03% PDGF and a chitosan sponge and one with a chitosan sponge alone-were placed. Microcomputed tomography and histologic sections were used to determine the amount of bone augmentation within the plastic caps. Bone volume was calculated using measurement software. Bone volume and amount of bone augmentation were significantly greater in the PDGF group than in the control group. In conclusion, a chitosan sponge containing 0.03% PDGF enhanced bone formation beyond the skeletal envelope in rat calvaria. (J Oral Sci 56, 23-28, 2014)

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A comparative study of microfocus CT and histomorphometry in the evaluation of bone augmentation in rat calvarium

Cited by 29 publications

References 17 publications

Effects of Platelet-Derived Growth Factor on Enhanced Bone Augmentation beyond the Skeletal Envelope within a Plastic Cap in the Rat Calvarium

Effects of Platelet-Derived Growth Factor on Enhanced Bone Augmentation beyond the Skeletal Envelope within a Plastic Cap in the Rat Calvarium

Regenerative capacity of augmented bone in rat calvarial guided bone augmentation model

Effect of a chitosan sponge impregnated with platelet-derived growth factor on bone augmentation beyond the skeletal envelope in rat calvaria

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