Histomorphometric, Immunohistochemical, Ultrastructural Characterization of a Nano-Hydroxyapatite/Beta-Tricalcium Phosphate Composite and a Bone Xenograft in Sub-Critical Size Bone Defect in Rat Calvaria

Brum, Igor da Silva; Frigo, Lúcio; Devita, Renan Lana; Pires, Jorge; Oliveira, Victor Hugo Vieira de; Nascimento, Ana Lúcia Rosa; Carvalho, Jorge José de

doi:10.3390/ma13204598

Cited by 17 publications

(15 citation statements)

References 33 publications

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“…As regards histometric analysis, the results of this work were different from those obtained by Mendes et al (34), who suggested the osteoconductive activity of the material, as it did not allow the development of bone tissue along the surface of the biomaterial; therefore, the clinical application must be such that the technique used and the indications for use be optimal for the material, fact also concluded in a comparative study between xenogenous biomaterials and synthetic nano-hydroxyapatite/beta-tricalcium phosphate composite (28,29,35).…”

Section: Critical Defectcontrasting

confidence: 95%

Biocompatibility and osteopromotor factor of bovine integral bone—a microscopic and histometric analysis

Bassi¹,

Bizelli²,

Consolaro³

et al. 2021

Front Oral Maxillofac Med

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Background: The objective of this study was to evaluate the biological properties, biocompatibility, and osteopromotor factor of integral bone of bovine origin implanted in critical defects of rat calvaria and the subcutaneous plane of rats. Methods:The study was divided into two stages. For the first stage, 24 rats were divided into two groups of 12 animals: group GC, in which the critical defect was filled only by clot, and group GO, in which the defect was filled with particulate biomaterial, and the analysis performed at 30 and 60 days postoperatively.For the second stage, 16 rats were divided into two groups of eight animals: the GOP group, in which the biomaterial in its particulate form was inserted in the subcutaneous plane, and the GOB group, in which the block biomaterial was inserted in the subcutaneous; and the analysis performed at 15 and 45 days postoperatively.Results: The histological and histometric results of the calvaria demonstrated that the biomaterial induced a foreign body reaction over the entire length of the defect and around the particles and was not able to induce bone neoformation. Statistically, no difference was observed for the time, biomaterial, and time × biomaterial parameters (P>0.05). Subcutaneous microscopic examination of the pieces obtained at 15 days showed an inflammatory reaction around the particles of the material with the presence of giant cells and at 45 days, and a reduction in the inflammatory reaction and presence of fibrous connective tissue around the particles was observed. with the presence of giant cells, and for the block biomaterial, connective tissue was present in the trabecular spaces. There was no evidence of ectopic bone formation. Conclusions:The biomaterial, despite being biocompatible, do not promote bone neoformation.

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Section: Critical Defectcontrasting

confidence: 95%

Biocompatibility and osteopromotor factor of bovine integral bone—a microscopic and histometric analysis

Bassi¹,

Bizelli²,

Consolaro³

et al. 2021

Front Oral Maxillofac Med

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“… 1 The determination of the percentage of phases is not yet well defined, although a proportion 80% HA and 20% β-TCP seem adequate because HA is a less resorbable framework than β-TCP, creating an ideal condition, where the reabsorption ratio versus bone matrix deposition keeps itself balanced, generating results in the quality of newly formed bone much more satisfactory than those found in other materials that present substrates of calcium phosphate in different concentrations. 33 The present results support these characteristics with an osteoinductive potential more relevant in the nano-HA/β-TCP mixture than in the DBBM group.…”

Section: Discussionsupporting

confidence: 84%

Performance of Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogenic Hydroxyapatite on Bone Regeneration in Rat Calvarial Defects: Histomorphometric, Immunohistochemical and Ultrastructural Analysis

Brum¹,

Frigo²,

Santos³

et al. 2021

IJN

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Background: Synthetic biomaterials have played an increasingly prominent role in the substitution of naturally derived biomaterials in current surgery practice. In vitro and in vivo characterization studies of new synthetic biomaterials are essential to analyze their physicochemical properties and the underlying mechanisms associated with the modulation of the inflammatory process and bone healing. Purpose: This study compares the in vivo tissue behavior of a synthetic biomaterial nano-hydroxyapatite/beta-tricalcium phosphate (nano-HA/ß-TCP mixture) and deproteinized bovine bone mineral (DBBM) in a rat calvarial defect model. The innovation of this work is in the comparative analysis of the effect of new synthetic and commercially xenogenic biomaterials on the inflammatory response, bone matrix gain, and stimulation of osteoclastogenesis and osteoblastogenesis. Methods: Both biomaterials were inserted in rat defects. The animals were divided into three groups, in which calvarial defects were filled with xenogenic biomaterials (group 1) and synthetic biomaterials (group 2), or left unfilled (group 3, controls). Sixty days after calvarial bone defects filled with biomaterials, periodic acid Schiff (PAS) and Masson's trichrome staining, immunohistochemistry tumor necrosis factoralpha (TNF-α), matrix metalloproteinase-9 (MMP-9), and electron microscopy analyses were conducted. Results: Histomorphometric analysis revealed powerful effects such as a higher amount of proteinaceous matrix and higher levels of TNF-α and MMP-9 in bone defects treated with alloplastic nano-HA/ß-TCP mixture than xenogenicxenogic biomaterial, as well as collagen-proteinaceous material in association with hydroxyapatite crystalloids. Conclusion:These data indicate that the synthetic nano-HA/ß-TCP mixture enhanced bone formation/remodeling in rat calvarial bone defects. The nano-HA/ß-TCP did not present risks of cross-infection/disease transmission. The synthetic nano-hydroxyapatite/beta-tricalcium phosphate mixture presented adequate properties for guided bone regeneration and guided tissue regeneration for dental surgical procedures.

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“…If PS-polarizing microscopy does not provide detailed collagen chemical characteristics, it does provide thorough access to the spatial architecture of the fiber’s arrangement and enables its quantitation. 16 , 17…”

Section: Discussionmentioning

confidence: 99%

Histomorphometric Evaluation of Bone-Guided Regeneration in Maxillary Sinus Floor Augmentation Using Nano-Hydroxyapatite/Beta-Tricalcium Phosphate Composite Biomaterial: A Case Report

Salviano¹,

Lopes²,

Brum³

et al. 2021

IMCRJ

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Background The development of techniques in biomaterials design and production added to advanced surgical procedures which enabled better and more predictable clinical outcomes. Maxillary sinus floor augmentation (MSFA) is among the more studied bone-guided regeneration procedure in the literature. The MSFA could be considered the gold standard procedure for bone-guided regeneration as it provides suitable functional and aesthetic solutions to alveolar ridge atrophy due to tooth loss. Purpose This study aimed to conduct a detailed histomorphometric evaluation of collagen production in SFAs bone-guided regeneration, using nano-hydroxyapatite/ß-tricalcium phosphate (nano-HA/ß-TCP) composite. Patients and Methods A 52-year-old female had the left upper second premolar condemned due to periodontal disease, then a tooth implant replacement was planned. Due to maxillary sinus pneumatization, the MSFA had to be done before implant placement. Nano-HA/ß-TCP composite (2g) was used in the MSFA procedure. After nine months of the healing process, during the Cone Morse implant installation process, bone samples were collected for histologic analysis (sirius red, hematoxylin/eosin, polarized microscopy). Six months after implant installation, a ceramic crown was installed according to the patient’s request. Results Proper masticatory function and aesthetics were re-established. The histomorphometric evaluation indicated that nano-HA/ß-TCP composite did not show any area devoid of cellular activity in sirius red or hematoxylin/eosin staining and the percentage (%) of new bone collagen fibers was achieved using polarization technique evaluation. Conclusion According to these results, nano-HA/ß-TCP composite presented clinical and histomorphometric properties suit to be used as bone-guided regeneration biomaterial in MSFA. Furthermore, nano-HA/β-TCP composite provided a favorable nano-environment to bone cells, enhancing bone matrix production.

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Histomorphometric, Immunohistochemical, Ultrastructural Characterization of a Nano-Hydroxyapatite/Beta-Tricalcium Phosphate Composite and a Bone Xenograft in Sub-Critical Size Bone Defect in Rat Calvaria

Cited by 17 publications

References 33 publications

Biocompatibility and osteopromotor factor of bovine integral bone—a microscopic and histometric analysis

Biocompatibility and osteopromotor factor of bovine integral bone—a microscopic and histometric analysis

Performance of Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogenic Hydroxyapatite on Bone Regeneration in Rat Calvarial Defects: Histomorphometric, Immunohistochemical and Ultrastructural Analysis

Histomorphometric Evaluation of Bone-Guided Regeneration in Maxillary Sinus Floor Augmentation Using Nano-Hydroxyapatite/Beta-Tricalcium Phosphate Composite Biomaterial: A Case Report

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