Histomorphometric Analysis of Bone Regeneration After Use of Propolis Versus Nanobone Graft Materials for the Management of Class Ii Furcation Defects in Dogs

Abstract

INTRODUCTION: Nanotechnology has been greatly utilized for bone regeneration strategies. It helps overcome some of the current limitations associated with bone regeneration methods Propolis is a natural substance made by the honeybee, it has effective antioxidant, antimicrobial, and anti-inflammatory effects. Moreover, it has multiple biological effects including improvement of wound healing and bone regeneration. OBJECTIVES: comparison of the effectiveness of NanoBone graft and propolis material on the inter-radicular bone regeneration in Mongrel dogs. MATERIALS AND METHODS: A split mouth design was carried out using the third and fourth mandibular premolars of 3 healthy mongrel dogs. A total of 12 grade II furcation defects were surgically created. The defects in the right side were treated with NanoBone graft and then a collagen membrane was used to cover the defects. In the left side the defects were treated with propolis and then covered with collagen membrane. The dogs were sacrificed after 4 weeks. Segments of the jaw bone containing the defects were then dissected out and prepared for histological evaluation. RESULTS: Both percentage of bone fill and percentage of bone surface area in defects treated with propolis powder were higher than those in NanoBone-filled defects. CONCLUSIONS: Both propolis and NanoBone graft materials have bone regenerative effects with higher ability of propolis to regenerate bone.

Applications of honeybee-derived products in bone tissue engineering

“…In fact, the histomorphometric and biomechanical results of chitosan were even superior to the autograft group when applied in combination with royal jelly ( Meimandi-Parizi et al, 2018b ). Additionally, comparisons have shown superior outcomes of propolis compared to NanoBone and Nanohydroxyapatite ( Zohery et al, 2018 ; Zohery et al, 2017 ).…”

“…Limitations of these studies included the lack of histological assessment in the early healing stage that could have revealed initial tissue reaction to propolis material, and also, the absence of clinical findings on the periodontal response to the grafted materials. Therefore, a clinical trial should be considered in future attempts ( Zohery et al, 2018 ; Zohery et al, 2017 ). Alcoholic extracts of propolis expedite tissue regeneration ( Sutta et al, 1975 ), enhance the healing process in damaged cartilage ( Scheller et al, 1977 ), and increase ossification in induced bone defects ( Stojko et al, 1978 ).…”

Effect of Botulinum Toxin on Surgical Wound Healing and Scar Formation (An Experimental Study)

“…For this reason, clinical research involving human subjects has traditionally been thought to require the use of animal experimental models. All the rabbits in the current study were male to exclude any variations that might be caused by female hormonal changes (5,13).…”

“…Particularly concerning is sepsis within 15 days of reconstitution (11,12). Anesthesia All surgical procedures were done under general anesthesia, with 0.05 ml of xylazine hydrochloride (Xyla-ject) (ADWIA, Egypt) and 0.1 ml of ketamine hydrochloride (Aneket) (NEON Pharmaceutical, UK) administered intramuscularly for every 100 grammes of body weight (13).…”

“…All specimens were labelled and fixed for one day in 10% neutral buffered formalin, after which segments that contained the surgical sites were dissected and left for five days in the same fixation (16). Histological procedures (13) Following fixation, specimens were cleaned, dehydrated in ethanol solutions with increasing ethanol concentrations, cleared with xylene, infiltrated, and embedded in paraffin wax. A rotatory microtome was used to cut thin sections in serial that were 2-5 m thick.…”