Abstract:Background: Periodontitis causes an increased receptor activator level in the nuclear factor-κβ ligand (RANKL), which is one of the inflammatory mediators that plays a role in osteoclastogenesis. The open flap debridement (OFD) technique is the preferred treatment when accompanied by regenerative periodontal treatment using guided tissue regeneration (GTR) and guided bone regeneration (GBR). Carbonated hydroxyapatite is a GBR material that serves as a scaffold and has strong osteoconductive properties for bone… Show more
“…Specifically, RANKL controls osteoclastogenesis in bone remodeling and modeling, respectively, on the surfaces of osteoblasts and osteocytes. 15,20 This study is also in line with a previous study that demonstrated how gelatin could regulate RANKL-induced osteoclast differentiation through TGF-β1 signaling pathways. 21 As noted, OPG and RANKL play a role as mutual competitors, which was confirmed through this study's results, which showed that there is an increase in the amount of OPG compared with RANKL and RANK.…”
Background: Osteoprotegerin (OPG) plays a role in preventing osteoclast formation and osteoclastic bone resorption by inhibiting the receptor activator nuclear factor-β and its ligand (RANK–RANKL) interaction that causes bone resorption. Pangasius djambal gelatin contains essential amino acids that may stimulate bone remodeling. Purpose: This study aimed to investigate the expressions of OPG, RANKL, and RANK following tooth extraction to ascertain whether OPG is associated with the RANKL and RANK ratios following gelatin induction. Methods: This study was a true experimental study with a simple, randomized post-test only control group design. Twenty-four albino rats (Rattus norvegicus) were divided into two groups (n = 4). The treatment group was treated with P. djambal gelatin following tooth extraction, while the control group was left untreated. Animal models undergoing tooth extraction were anesthetized intraperitoneally with ketamine (1,000 mg/10 ml up to 0.2 ml). The tooth was then extracted slowly so as not to break it. P. djambal gelatin was dripped (0.1 ml) into the socket after the extraction procedure. The association of OPG with RANKL and RANK immunohistochemistry expressions was evaluated after three, five, and seven days. Results: There was a significant increase in OPG expression on day 7 (P < 0.05) and a significant decrease in the expression of RANKL and RANK (P < 0.05) in the treatment group. Conclusion: The application of P. djambal gelatin could increase OPG expression, decrease RANKL and RANK expression in the socket following tooth extraction, and might be associated with the inhibition of bone resorption.
“…Specifically, RANKL controls osteoclastogenesis in bone remodeling and modeling, respectively, on the surfaces of osteoblasts and osteocytes. 15,20 This study is also in line with a previous study that demonstrated how gelatin could regulate RANKL-induced osteoclast differentiation through TGF-β1 signaling pathways. 21 As noted, OPG and RANKL play a role as mutual competitors, which was confirmed through this study's results, which showed that there is an increase in the amount of OPG compared with RANKL and RANK.…”
Background: Osteoprotegerin (OPG) plays a role in preventing osteoclast formation and osteoclastic bone resorption by inhibiting the receptor activator nuclear factor-β and its ligand (RANK–RANKL) interaction that causes bone resorption. Pangasius djambal gelatin contains essential amino acids that may stimulate bone remodeling. Purpose: This study aimed to investigate the expressions of OPG, RANKL, and RANK following tooth extraction to ascertain whether OPG is associated with the RANKL and RANK ratios following gelatin induction. Methods: This study was a true experimental study with a simple, randomized post-test only control group design. Twenty-four albino rats (Rattus norvegicus) were divided into two groups (n = 4). The treatment group was treated with P. djambal gelatin following tooth extraction, while the control group was left untreated. Animal models undergoing tooth extraction were anesthetized intraperitoneally with ketamine (1,000 mg/10 ml up to 0.2 ml). The tooth was then extracted slowly so as not to break it. P. djambal gelatin was dripped (0.1 ml) into the socket after the extraction procedure. The association of OPG with RANKL and RANK immunohistochemistry expressions was evaluated after three, five, and seven days. Results: There was a significant increase in OPG expression on day 7 (P < 0.05) and a significant decrease in the expression of RANKL and RANK (P < 0.05) in the treatment group. Conclusion: The application of P. djambal gelatin could increase OPG expression, decrease RANKL and RANK expression in the socket following tooth extraction, and might be associated with the inhibition of bone resorption.
“…Interestingly, propolis was shown to reduce osteoclastogenesis by modulating RANKL expression. Devitaningtyas et al (2020) found that the RANKL expression in rabbit’s alveolar bones was reduced by the propolis-incorporated with carbonated hydroxyapatite.…”
Propolis is a resinous product collected by bees from plant exudates to protect and maintain hive homeostasis. Propolis has been used therapeutically for centuries as folk medicine. Modern research investigating the diversity of the chemical composition and plant sources, biological activity, extraction processes, analytical methods, and therapeutic properties in clinical settings have been carried out extensively since the 1980s. Due to its antimicrobial, anti-inflammatory, and immuno-modulator properties, propolis appears to be a suitable bioactive component to be incorporated into biomaterials. This review article attempts to analyze the potential application of propolis as a biomaterial component from the available experimental evidence. The efficacy and compabitility of propolis depend upon factors, such as types of extracts and types of biomaterials. Generally, propolis appears to be compatible with hydroxyapatite/calcium phosphate-based biomaterials. Propolis enhances the antimicrobial properties of the resulting composite materials while improving the physicochemical properties. Furthermore, propolis is also compatible with wound/skin dressing biomaterials. Propolis improves the wound healing properties of the biomaterials with no negative effects on the physicochemical properties of the composite biomaterials. However, the effect of propolis on the glass-based biomaterials cannot be generalized. Depending on the concentration, types of extract, and geographical sources of the propolis, the effect on the glass biomaterials can either be an improvement or detrimental in terms of mechanical properties such as compressive strength and shear bond strength. In conclusion, two of the more consistent impacts of propolis across these different types of biomaterials are the enhancement of the antimicrobial and the immune-modulator/anti-inflammatory properties resulting from the combination of propolis and the biomaterials.
“…Limitations of this study was a short duration of the experiment. It was essential to conduct more research on the impact of the application of 10 % propolis and carbonate hydroxyapatite in OFD with a longer duration of observation ( Devitaningtyas et al, 2020 ). Fig.…”
Section: Propolismentioning
confidence: 99%
“…( Pereira et al, 2012 ). Additional findings have indicated that propolis can decrease osteoclastogenesis when administered in combination with carbonated hydroxyapatite ( Devitaningtyas et al, 2020 ).…”
Section: Propolismentioning
confidence: 99%
“…The efficacy of propolis in healing femoral defects has also been demonstrated when implants loaded with propolis were used in conjunction with propolis treatment in rats ( Guney et al, 2011 ). Coating implants with propolis has been shown to improve bone formation ( Devitaningtyas et al, 2020 ; Boudra, 2014 ; Pereira et al, 2012 ; Al-Molla et al, 2014 ). Furthermore, new electrospun nanocomposite scaffolds consisting of polyurethane loaded with ghee and propolis have been developed, exhibiting suitable physicochemical properties, blood compatibility, and non-toxicity, making them potential candidates for bone tissue engineering ( Mani and Jaganathan, 2020 ).…”
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