Histology of the rat vas deferens after injection of a non-occlusive chemical contraceptive

Verma, Kushagra; Misro, M. M.; Singh, Harpal; Mahajan, Sudha; Ray, Alok R.; Guha, Sujoy K.

doi:10.1530/jrf.0.0630539

Cited by 21 publications

(12 citation statements)

References 3 publications

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“…The feasibility of reversal of SMA vas occlusion by DMSO and sodium bicarbonate has also been demonstrated earlier (Verma et al. , 1981; Koul et al.…”

Section: Discussionsupporting

confidence: 58%

“…Although non‐invasive reversal has been successfully demonstrated in langur monkeys, the procedure may be less feasible in human because the human vas takes a deep course in the inguinal region, which makes it more difficult to palpate the vas beyond the scrotum. The feasibility of preliminary trial of SMA reversal by DMSO (Verma et al. , 1981) is under consideration as an alternative approach to non‐invasive reversal.…”

Section: Introductionmentioning

confidence: 99%

“…Although non-invasive reversal has been successfully demonstrated in langur monkeys, the procedure may be less feasible in human because the human vas takes a deep course in the inguinal region, which makes it more difficult to palpate the vas beyond the scrotum. The feasibility of preliminary trial of SMA reversal by DMSO (Verma et al, 1981) is under consideration as an alternative approach to non-invasive reversal. In this study, an attempt has been made to establish the functional success and safety of reversal of RISUG vas occlusion by DMSO through sperm characteristics, fertility status and teratological changes, if any, in F 1 offspring of the reversed animals in albino rats.…”

Section: Introductionmentioning

confidence: 99%

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Sperm characteristics and teratology in rats following vas deferens occlusion with RISUG and its reversal

Lohiya¹,

Suthar²,

Khandelwal³

et al. 2010

Int J of Andrology

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The functional success of the reversal of vas occlusion by styrene maleic anhydride (RISUG), using the solvent vehicle, Dimethyl Sulphoxide (DMSO), has been investigated. Reversal with DMSO was carried out in Wistar albino rats 90 days after bilateral vas occlusion. The body weight, organ weight, sperm characteristics, fertility test and teratology, including skeletal morphology were evaluated in vas occlusion and reversal animals and in F(1) progenies to assess the functional success of the occlusion and reversal. Body weight, organ weight and the cauda epididymal sperm characteristics of vas occlusion and reversal animals and of F(1) progenies were comparable to control. Ejaculated spermatozoa in the vaginal smear showed detached head/tail, acrosomal damage, bent midpiece, bent tail and morphological aberrations in sperm head after vas occlusion, which returned to normal, 90 days after reversal. Monthly fertility test, post-injection showed 0% fertility, which improved gradually and 100% fertility was achieved 90 days after reversal. The fertility/pregnancy/implantation record and skeletal morphology of the offspring were comparable to control. The results suggest functional success and safety of vas occlusion reversal by DMSO.

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“…The feasibility of reversal of SMA vas occlusion by DMSO and sodium bicarbonate has also been demonstrated earlier (Verma et al. , 1981; Koul et al.…”

Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sperm characteristics and teratology in rats following vas deferens occlusion with RISUG and its reversal

Lohiya¹,

Suthar²,

Khandelwal³

et al. 2010

Int J of Andrology

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show abstract

“…9 Just after injection, RISUG lowers the pH suffi ciently to kill the sperm passing through. 32,33 RISUG is presently undergoing extended phase III clinical trials in India 2,34 after successfully completing phase I and phase II trials.…”

Section: Discussionmentioning

confidence: 99%

“…2 It consists of a hydrogel preparation of copolymer styrene maleic anhydride (SMA) dissolved in dimethyl sulphoxide (DMSO) possessing antimicrobial properties. 3,4 Long-term vas occlusion with RISUG (SMA-DMSO) has been proved safe [5][6][7][8][9] and effective. [10][11][12][13] Reversal by injection of sodium bicarbonate solution as solvent has been demonstrated in the rat.…”

Section: Introductionmentioning

confidence: 99%

Smart RISUG: A potential new contraceptive and its magnetic field-mediated sperm interaction

Jha¹,

Jha²,

Guha³

2009

IJN

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Abstract:The rationale and technique underlying a novel concept of noninvasive fertility control by a new Cuproferrogel contraceptive drug, iron oxide-copper-styrene maleic anhydride-dimethyl sulphoxide (Fe 3 O 4 -Cu-SMA-DMSO) composite named 'Smart RISUG' (smart reversible inhibition of sperm under guidance) in presence of pulsed magnetic fi eld (PMF; 1 mT to 800 mT) is explained. It was synthesized by dispersing iron oxide particles and copper particles into SMA-DMSO (male contraceptive RISUG) and characterized for particle distribution, particle size measurement and transmittance peaks, etc. Interaction of the RISUG particles as well as Smart RISUG particles with Albino rat sperm cell was studied in presence as well as absence of PMF. To fi nd an explanation to increased reaching of the Smart RISUG drug into sperm under infl uence of magnetic fi eld, the transport properties were characterized by high resolution transmission electron microscopy and atomic force microscopy. Smart RISUG could be mobilized into sperm cell membrane at the PMF, 760 mT in about 50 seconds. Adoption of novel drug Smart RISUG involving new technique may open the pathway for non surgical control of drug distribution, detection and restoration of the normal fertility after removal of the contraceptive from the male/female reproductive tube in presence of electromagnetic fi eld.

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Modification of LLDPE using esterified styrene maleic anhydride copolymer: Study of its properties and environmental degradability

Pal

Singh

Ghosh

2004

J of Applied Polymer Sci

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Linear low-density polyethylene (LLDPE) was blended with decanol-esterified styrene maleic anhydride copolymer (MDESMA) with an aim to enhance the environmental degradability of polyethylenes. Styrene-maleic anhydride copolymer (SMA) was synthesized by precipitation polymerization, using benzoyl peroxide (BPO) as initiator. SMA was esterified with a long-chain monoalcohol, n-decanol, using methyl ethyl ketone (MEK) as solvent at 80°C to obtain monoesterified styrene-maleic anhydride (MDESMA). Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimeter (DSC), and thermogravimetric analysis (TGA) were performed to characterize SMA and MDESMA. IR spectra of MDESMA showed a decrease in intensity of peak responsible for carbonyl absorption of a five-membered ring anhydride group along with broadening of carboxyl O-H stretching peak. TGA showed two-stage degradation for SMA and MDESMA. LL-DPE was blended with MDESMA in single-screw extruder and blends were characterized thermally by DSC and TGA. A single endothermic melting peak of LLDPE/MDESMA blend was observed. Films of the blends, formed by compression molding, showed an increase in modulus of elasticity but a decrease in elongation at break with increasing concentration of MDESMA. LLDPE/MDESMA blend compositions when kept in phosphate/citric acid buffer solution (pH ϳ 8) showed initial weight gain because of water absorption and subsequently loss in weight due to dissolution of soluble component of blends. Film samples of blends kept for soil burial also showed similar behavior. Contact-angle measurement of film samples of the blends showed an increase in value on soil burial, indicating degradation/dissolution of MDESMA

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Histology of the rat vas deferens after injection of a non-occlusive chemical contraceptive

Cited by 21 publications

References 3 publications

Sperm characteristics and teratology in rats following vas deferens occlusion with RISUG and its reversal

Sperm characteristics and teratology in rats following vas deferens occlusion with RISUG and its reversal

Smart RISUG: A potential new contraceptive and its magnetic field-mediated sperm interaction

Modification of LLDPE using esterified styrene maleic anhydride copolymer: Study of its properties and environmental degradability

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