Histology of spheroidal degeneration of the cornea in Labrador.

Johnson, Gordon J.; Overall, Mary

doi:10.1136/bjo.62.1.53

Cited by 43 publications

(20 citation statements)

References 18 publications

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“…Climatic droplet keratopathy is relatively unusual and more often reported in harsh climates, such as arctic or desert areas (Klintworth 1977). Ultraviolet radiation is thought to play a role in its etiology (Taylor 1980;Johnson & Ghosh 1975;Johnson & Overall 1978;Dahan et al 1986;Clear et al 1979). Recently, climatic proteoglycan stromal keratopathy in the Middle East has been described by Waring et al (1995).…”

Section: Discussionmentioning

confidence: 99%

Repeated UVR exposures cause keratocyte resistance to apoptosis and hyaluronan accumulation in the rabbit cornea

Podskochy

Fagerholm

2001

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To evaluate hyaluronan (HA) production and level of apoptosis of corneal cells after repeated UVR exposures. Methods: Fifteen albino rabbit corneas were exposed to 310 nm UVR at a dose that causes biomicroscopically significant keratitis (0.47 J/cm 2 ). Nine rabbits received a single dose of UVR. Six rabbits were irradiated 3 times at 7-day intervals. Rabbits exposed to the single dose of UVR, were sacrificed 24 hours, 7 and 14 days after irradiation. Rabbits exposed to the repeated doses of UVR, were sacrificed 24 hours and 14 days after the last irradiation. The corneal tissue specimens were processed for histological analysis using specific staining for HA, and the TdT-dUTP terminal nick-end labeling (TUNEL) assay. Results: Corneas exposed to a single UVR dose showed extensive positive TU-NEL staining 24 hours after exposure. Almost all basal epithelial cells, keratocytes throughout the entire thickness of the stroma, and endothelial cells were TUNEL-positive. No HA was found 24 hours after exposure. Extracellular HA staining of high intensity was found at day 7 throughout the entire central stroma, except the anterior one-fourth. At day 14 only a faint HA staining was detected in the posterior stroma, close to Descemet's membrane. Corneas exposed to repeated UVR doses showed at 24 hours positive TUNEL staining only in epithelial cells and in very few stromal cells. The majority of stromal cells and endothelial cells were unaffected. At the same time HA staining of very high intensity was found both at 24 hours and day 14, and it was evenly distributed throughout the entire thickness of the stroma. Conclusion: Repeated UVR exposures lead to increased production and accumulation of HA in the corneal stroma. The repopulated keratocytes are much more resistant to apoptosis than the native ones. HA accumulation may be a sign of long-term changes in the cornea.

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Section: Discussionmentioning

confidence: 99%

Repeated UVR exposures cause keratocyte resistance to apoptosis and hyaluronan accumulation in the rabbit cornea

Podskochy

Fagerholm

2001

Acta Ophthalmologica Scandinavica

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“…The latter may be of altered plasma constituents from leaking limbal vessels. 22 The accepted theories of collagen degeneration in both lattice dystrophy and corneal elastosis suggest the possibility of a closely related metabolic reason for both, although one is genetically inherited and the other is not. Corneal elastosis may be associated with a variety of chronic ocular and corneal disorders.7" Our patients presented clinical findings or a history of other corneal diseases like those of other cases with corneal elastosis.…”

Section: Discussionmentioning

confidence: 99%

Corneal elastosis within lattice dystrophy lesions.

Pe'er

Fine

Dixon

et al. 1988

British Journal of Ophthalmology

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“…The coalescence and increased volume of these spherules or deposits may cause disruption of Bowman’s membrane and elevation and thinning of the corneal epithelium [1,8,9,10]. Electron microscopy has shown that globules are round, electron-dense and sharply demarcated structures, always surrounded by basement membrane material, and adjacent disorganized collagen fibrils [1,9]. The droplets generally lack positive staining for fat or calcium [1].…”

Section: Introductionmentioning

confidence: 99%

“…The droplets generally lack positive staining for fat or calcium [1]. Although the exact nature and composition of droplets remain unclear, proteinaceous constituents have been found [8,9,10,11]. …”

Section: Introductionmentioning

confidence: 99%

Genetic Background and Climatic Droplet Keratopathy Incidence in a Mapuche Population from Argentina

et al. 2013

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PurposeTo determine whether the incidence of and susceptibility to climatic droplet keratopathy (CDK), an acquired, often bilateral degenerative corneal disease, is influenced by the genetic background of the individuals who exhibit the disorder.MethodsTo determine whether the disease expression was influenced by the genetic ancestry of CDK cases in native Mapuche of the northwest area of Patagonia in Argentina, we examined mitochondrial DNA and Y-chromosome variation in 53 unrelated individuals. Twenty-nine of them were part of the CDK (patient) population, while 24 were part of the control group. The analysis revealed the maternal and paternal lineages that were present in the two study groups.ResultsThis analysis demonstrated that nearly all persons had a Native American mtDNA background, whereas 50% of the CDK group and 37% of the control group had Native American paternal ancestry, respectively. There was no significant difference in the frequencies of mtDNA haplogroups between the CDK patient and control groups. Although the Y-chromosome data revealed differences in specific haplogroup frequencies between these two groups, there was no statistically significant relationship between individual paternal genetic backgrounds and the incidence or stage of disease.ConclusionsThese results indicate a lack of correlation between genetic ancestry as represented by haploid genetic systems and the incidence of CDK in Mapuche populations. In addition, the mtDNA appears to play less of a role in CDK expression than for other complex diseases linked to bioenergetic processes. However, further analysis of the mtDNA genome sequence and other genes involved in corneal function may reveal the more precise role that mitochondria play in the expression of CDK.

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Histology of spheroidal degeneration of the cornea in Labrador.

Cited by 43 publications

References 18 publications

Repeated UVR exposures cause keratocyte resistance to apoptosis and hyaluronan accumulation in the rabbit cornea

Repeated UVR exposures cause keratocyte resistance to apoptosis and hyaluronan accumulation in the rabbit cornea

Corneal elastosis within lattice dystrophy lesions.

Genetic Background and Climatic Droplet Keratopathy Incidence in a Mapuche Population from Argentina

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