Histological Techniques for Decalcified Bone and Cartilage

Kang, Qian; LaBreck, Jackie C.; Gruber, Helen E.; An, Yuehuei H.

doi:10.1385/1-59259-417-4:209

Cited by 2 publications

(1 citation statement)

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“…Histological pathological sections of femurs and tibias were as previously described [22] . Briefly, femurs and tibias were fixed in paraformaldehyde, then decalcified by immersion in Servicebio EDTA decalcified solution (Shanghai, China).…”

Section: Histological Examinationmentioning

confidence: 99%

Bifidobacterium breveCCFM1078 regulates bone development in growing BALB/c mice via gut microbiota and metabolites to balance bone formation and resorption

Li,

Ding,

Liu

et al. 2024

Food Science and Human Wellness

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This study endeavors to investigate the effects of Bifidobacterium breve CCFM1078 on bone formation and resorption balance in growing BALB/c mice. Newborn BALB/c mice were assigned to the control group (administration saline) and the CCFM1078 group (administration B. breve CCFM1078, 3 × 10 9 CFU/day) in 3-, 4-, and 5-week tests. All the groups have male and female distinctions. Our findings demonstrate that B. breve CCFM1078 exerts on the dynamic equilibrium between bone formation and resorption during the critical period of growth in mice by modulating the composition of gut microbiota and metabolites (hexadecanamide, linoleoyl ethanolamide, and palmitoyl ethanolamide), the genes and proteins expression related to the GH/IGF-1 axis and Gs/PKA/CREB signaling pathways, as well as downstream osteogenic and osteoclastic differentiation factors. The effects of B. breve CCFM1078 were different with age and gender dependent. This finding suggests B. breve CCFM1078 may have potential applications in regulating bone metabolism in the growth period population.

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Section: Histological Examinationmentioning
confidence: 99%

Bifidobacterium breveCCFM1078 regulates bone development in growing BALB/c mice via gut microbiota and metabolites to balance bone formation and resorption
Li,
Ding,
Liu
et al. 2024
Food Science and Human Wellness
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0
0
0
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Injectable perlecan domain 1-hyaluronan microgels potentiate the cartilage repair effect of BMP2 in a murine model of early osteoarthritis
Srinivasan
¹
,
McCoy
²
,
Jha
³

et al. 2012
Biomed. Mater.
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The goal of this study was to use bioengineered injectable microgels to enhance the action of bone morphogenetic protein 2 (BMP2) and stimulate cartilage matrix repair in a reversible animal model of osteoarthritis (OA). A module of perlecan (PlnD1) bearing heparan sulfate (HS) chains was covalently immobilized to hyaluronic acid (HA) microgels for the controlled release of BMP2 in vivo. Articular cartilage damage was induced in mice using a reversible model of experimental OA and was treated by intra-articular injection of PlnD1-HA particles with BMP2 bound to HS. Control injections consisted of BMP2 free PlnD1-HA particles, HA particles, free BMP2 or saline. Knees dissected following these injections were analyzed using histological, immunostaining and gene expression approaches. Our results show that knees treated with PlnD1-HA/BMP2 had lesser OA-like damage compared to control knees. In addition, the PlnD1-HA/BMP2-treated knees had higher mRNA levels encoding for type II collagen, proteoglycans, and xylosyltransferase 1, a rate-limiting anabolic enzyme involved in the biosynthesis of glycosaminoglycan chains, relative to control knees (PlnD1-HA). This finding was paralleled by enhanced levels of aggrecan in the articular cartilage of PlnD1-HA/BMP2 treated knees. Additionally, decreases in the mRNA levels encoding for cartilage-degrading enzymes and type X collagen were seen relative to controls. In conclusion, PlnD1-HA microgels constitute a formulation improvement compared to HA for efficient in vivo delivery and stimulation of proteoglycan and cartilage matrix synthesis in mouse articular cartilage. Ultimately, PlnD1-HA/BMP2 may serve as an injectable therapeutic agent for slowing or inhibiting the onset of OA after knee injury.

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Histological Techniques for Decalcified Bone and Cartilage

Cited by 2 publications

References 0 publications

Bifidobacterium breveCCFM1078 regulates bone development in growing BALB/c mice via gut microbiota and metabolites to balance bone formation and resorption

Bifidobacterium breveCCFM1078 regulates bone development in growing BALB/c mice via gut microbiota and metabolites to balance bone formation and resorption

Injectable perlecan domain 1-hyaluronan microgels potentiate the cartilage repair effect of BMP2 in a murine model of early osteoarthritis

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