Histological study of the influence of antidiabetic plant collection on pancreatic tissue in rats with diabetes mellitus, which is induced by dexamethasone

Abstract

Diabetes mellitus is a global problem today, because there is a sharp increase the number of patients in the world each year. Severe complications of diabetes are very dangerous because it can lead to disability of patients and high mortality.
 The aim of our work was to study the protective effect of antidiabetic plant collection on the pancreas in rats with diabetes type 2, caused by the introduction of dexamethasone.
 The research materials were pancreatic tissue of rats with experimental diabetes that received the decoction (1:10) of antidiabetic plant collection (witch includes Equiseti arvensis herba, Sambuci flores, Inulae rhizomata et radices, Hyperici herba, Tiliae flores, Polygoni avicularis herba, Myrtilli folium, Urticae folia) in treatment and preventive mode. The model of diabetes mellitus was induced by administration of dexamethasone 0.125 ml/kg for 15 days.
 Decoction of antidiabetic plant collection in doses 6 ml/kg, 9 ml/kg and 12 ml/kg reduces diabetogenic properties of dexamethasone when administered simultaneously. The insular tissue increases absorbance pancreatic islets, to some extent restored the normal percentage distribution of the islets in size, histologically visualized the increase more high-grade cells.
 The study found that antidiabetic plant collection reduces destructive-dystrophic changes of insular apparatus in rats with diabetes type 2. Antidiabetic effect of the studied plant collection has dose-dependent nature. The most effective conditional-effective doses are 9 and 12 ml/kg.

Sorghum flour's effect on improving plasma lipid profile and atherogenic index in diabetic rats

“…The decrease in mean weight during the intervention in the (K+) group was due to this group's insulin resistance and not receiving nutritional therapy. Insulin resistance causes the body to be unable to absorb glucose in the blood optimally and disrupts the glycolysis process, triggering the glycogenolysis process (Savych & Marchyshyn., 2017). The process of glycogenolysis that occurs continuously can cause weight loss.…”

“…The highest average feed consumption was in the (K+) group, but there was a decrease in body weight in this group. Because the (K+) group experienced insulin resistance which caused the glycogenolysis process (Savych & Marchyshyn, 2017), the situation will cause leptin's performance in controlling appetite in the hypothalamus and brain stem from being disrupted because the body's cells do not receive glucose by insulin performance, so the body will assume that it still needs energy and continues to feel hungry. The body then produces energy by burning fat and muscle.…”

“…Insulin resistance causes a decrease in the functional activity of cells so that in conditions of insulin resistance or insulin deficiency, it can interfere with the glycolysis process due to a decrease in the work of the enzymes glucokinase, phosphofructokinase, and pyruvate kinase. (Lenzen, 2014;Savych and Marchyshyn., 2017) Insulin resistance causes Hormone Sensitive Lipase (HSL) to be active, thereby increasing the lipolysis of triglycerides in adipose tissue to meet energy needs through gluconeogenesis. This situation causes excess free fatty acids, used as raw materials for formation (Nuraini et al, 2017).…”