Histological Study of Fresh Versus Frozen Semitendinous Muscle Tendon Allografts

Bitar, Alexandre Carneiro; Santos, Luiz Alberto dos; Croci, Alberto Tesconi; Maradei-Pereira, João Alberto Ramos; Bisneto, Edgard Novaes França; Giovani, Arlete Mazzini Miranda; Oliveira, Cláudia de

doi:10.1590/s1807-59322010000300010

Cited by 21 publications

(9 citation statements)

References 32 publications

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“…While literature provides comparative analysis of the fresh and fresh-frozen tendons [18,6], bones [10,26,41], osteochondral allografts [29] there is lack of such comparison for the human peripheral nerves. Hohmann et al [18] revealed that the long head of biceps tendons showed higher loads to failure and lower elasticity in the fresh-frozen samples when compared to the fresh specimens.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the histological structure of the tibial nerve and its terminal branches in the fresh and fresh-frozen cadavers

et al. 2021

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Background: The aim of this study was to compare the histological structure (cross-sectional area (CSA) and number of nerve fascicles) of the distal part of the tibial nerve (TN) and its terminal branches (medial plantar nerve [MPN], lateral plantar nerve [LPN]) in the fresh and fresh-frozen cadavers using computer assisted image analysis. Materials and methods: The tibial nerve with terminal branches (medial and lateral plantar nerves) were dissected from the fresh and fresh-frozen cadavers. Each nerve was harvested 5 mm proximally and respectively 5 mm distally from the tibial nerve bifurcation, marked, dehydrated, embedded in paraffin, sectioned at 2 µm slices and stained with haematoxylin and eosin. Then the specimens were photographed and analyzed using Olympus cellSens software. Results: The fresh cadavers group comprised 60 feet (mean age 68.1 ± 15.2 years). The mean CSA and the number of nerve fascicles were respectively 15.25 ± 4.6 mm 2 , 30.35 ± 8.45 for the tibial nerve, 8.76 ± 1.93 mm 2 , 20.75 ± 7.04 for the medial plantar nerve and 6.54 ± 2.02 mm 2 , 13.40 ± 5.22 for the lateral plantar nerve. The fresh-frozen cadavers group comprised 21 feet (mean age 75.1 ± 9.0 years). The mean CSA and the number of nerve fascicles were respectively 13.71 ± 5.66 mm 2 , 28.57 ± 8.00 for the tibial nerve, 7.55 ± 3.25 mm 2 , 18.00 ± 6.72 for the medial plantar nerve and 4.29 ± 1.93 mm 2 , 11.33 ± 1.93 for the lateral plantar nerve. Only lateral plantar nerves showed statistical differences in the CSA and the number of nerve fascicles between examined groups (p = 0.000, p = 0.037 respectively). A positive correlation was found between donors age and tibial nerve CSA in the fresh cadavers group (r = 0.44, p = 0.000). A statistical difference was found between the medial and lateral plantar nerves both in the CSA and the number of nerve fascicles (p < 0.001, p < 0.001 respectively). Conclusions: The CSA and the number of nerve fascicles of the tibial and medial plantar nerves were similar in the fresh and fresh-frozen cadavers whilst different in the lateral plantar nerve. The tibial nerve showed increasing CSA with the advanced age in the fresh cadavers. The medial plantar nerve had larger CSA and more nerve fascicles than the lateral plantar nerve.

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Section: Discussionmentioning

confidence: 99%

Comparison of the histological structure of the tibial nerve and its terminal branches in the fresh and fresh-frozen cadavers

et al. 2021

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“…Cartner et al [2011] noted that specimen exposure time after thawing has a detrimental effect on tissue properties. In contrast, other researchers could not find any evidence that the physical and histological properties of fresh frozen specimens were altered during cold storage [Panjabi et al, 1985;Bitar et al, 2010].…”

Section: Introductionmentioning

confidence: 91%

The mechanical properties of fresh versus fresh/frozen and preserved (Thiel and Formalin) long head of biceps tendons: A cadaveric investigation

Hohmann

Keough

Glatt

et al. 2019

Annals of Anatomy - Anatomischer Anzeiger

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Human cadaveric specimens commonly serve as mechanical models and as biological tissue donors in basic biomechanical research. Although these models are used to explain both in vitro and in vivo behavior, the question still remains whether the specimens employed reflect the normal in vivo situation. The mechanical properties of fresh-frozen or preserved cadavers may differ, and whether they can be used to reliably investigate pathology could be debated. The purpose of this study was to therefore examine the mechanical properties of cadaveric long biceps tendons, comparing fresh (n=7) with fresh-frozen (n=8), formalin embalmed (n=15), and Thiel-preserved (n=6) specimens using a Universal Testing Machine. The modulus of elasticity and the ultimate tensile strength to failure was recorded. Tensile failure occurred at an average of 12N/mm in the fresh group, increasing to 40.1N/mm in the fresh-frozen group, 50.3N/mm in the formalin group, and 52N/mm in the Thiel group. The modulus of elasticity/stiffness of the tendon increased from fresh (25.6MPa), to fresh-frozen (55.3MPa), to Thiel (82.5MPa), with the stiffest being formalin (510.6MPa). Thiel-preserved and formalin-embalmed long head of biceps tendons and fresh-frozen tendons have a similar load to failure. Either the Thiel or formalin preserved tendon could therefore be considered as alternatives for load to failure studies. However, the Young's modulus of embalmed tendons were significantly stiffer than fresh or fresh frozen specimens, and these methods might be less suitable alternatives when viscoelastic properties are being investigated.

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“…He was from the neighboring country and would not stay for long. When a decision to do a staged operation was decided during operation, we did not have a tissue bank or facilities for cryopreservation of the harvested tendons at −80 °C or with liquid nitrogen at −179 °C yet we had to keep the harvested tendons safe [ 11 ]. We opted to keep them in situ as the best option in our situation.…”

Section: Discussionmentioning

confidence: 99%

A case report of anterior cruciate ligament and posterolateral corner reconstruction using tendon graft preserved in situ

Mpatswenumugabo

Bukara

Semakula

et al. 2018

International Journal of Surgery Case Reports

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Histological Study of Fresh Versus Frozen Semitendinous Muscle Tendon Allografts

Cited by 21 publications

References 32 publications

Comparison of the histological structure of the tibial nerve and its terminal branches in the fresh and fresh-frozen cadavers

Comparison of the histological structure of the tibial nerve and its terminal branches in the fresh and fresh-frozen cadavers

The mechanical properties of fresh versus fresh/frozen and preserved (Thiel and Formalin) long head of biceps tendons: A cadaveric investigation

A case report of anterior cruciate ligament and posterolateral corner reconstruction using tendon graft preserved in situ

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