Histological observation of cucumber infected with Corynespora cassiicola

Liu, Dong; Qin, Zhiwei; Zhang, Yanju; Zhou, Xiuyan; Xin, Ming

doi:10.1007/s10658-017-1195-8

Cited by 13 publications

(13 citation statements)

References 35 publications

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“…Corynespora cassiicola can infect more than 500 crops, including rubber tree (Déon et al., 2012), cucumber (Liu et al., 2017) and scarlet sage (Furukawa et al., 2008). Researchers have used GFP‐tagged Corynespora cassiicola isolates to visualize different stages of infection (Liu et al., 2014).…”

Section: Discussionmentioning

confidence: 99%

Cell‐wall‐degrading enzymes produced by sesame leaf spot pathogen Corynespora cassiicola

Jiang

Liu

et al. 2020

Journal of Phytopathology

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Corynespora leaf spot (CLS) is one of the most important diseases in sesame and is caused by a fungal pathogen, Corynespora cassiicola. In this study, we investigated the role of cell‐wall‐degrading enzymes (CWDEs) in the process of pathogen infection and aimed to identify the enzyme that exerts positive effect. Corynespora cassiicola was inoculated in liquid culture and in sesame leaf. At 7 days postinoculation, CWDEs including polygalacturonase (PG), cellulase (CL), neutral xylanase (NEX) and laccase (LC) were detected in the supernatant and leaf extracts inoculated with Corynespora cassiicola. The result showed that the activities of LC and NEX were higher in liquid culture, while the activities of CL and PG were lower. In the sesame leaf infected by Corynespora cassiicola, the activity of NEX was the highest, those of CL and LC were the second highest and that of PG was the weakest, which indicated that NEX was the key enzyme in the process of sesame infection by Corynespora cassiicola. Secondly, the activities of the CWDEs secreted by Corynespora cassiicola were disparate in different sesame varieties. CWDEs activities of cultivar Jz were generally higher than cultivar Hn, and the activity change curve of Jz was also steeper than that of Hn. This indicates that the resistance mechanism of Hn may inhibit the activities of CWDEs and that the appearance and expansion of leaf necrosis spot is related to the comprehensive accumulation and action of various CWDEs. This is the first report investigating the association between CWDEs and pathogenesis of Corynespora cassiicola, causing Corynespora leaf spot in sesame.

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Section: Discussionmentioning

confidence: 99%

Cell‐wall‐degrading enzymes produced by sesame leaf spot pathogen Corynespora cassiicola

Jiang

Liu

et al. 2020

Journal of Phytopathology

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“…Spore of cucumber pathogen C. cassiicola could be germinated from one end or both ends of the spore at 25-30℃ with above 90% of relative humidity, and the germination rate in water drop was the highest. C. cassiicola was able to invade into cucumber leaves mainly directly or via stomata [6]. C. cassiicola showed pathogenic and genetic variation among isolates sampled in different host plants, proving that the intraspecific strains of C. cassiicola showed high host specialization [2,7].…”

Section: Introductionmentioning

confidence: 97%

Genome sequence and spore germination associated transcriptome analysis of Corynespora cassiicola from cucumber

Gao

Zeng

Suo

et al. 2019

Preprint

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Corynespora cassiicola as a necrotrophic plant pathogenic ascomycetes fungus can infect hundreds of species of plants, and also rarely cause human disease. The pathogen infects cucumber and causes cucumber target spot, which has given rise to great yield loss of cucumber in China recently. Genome sequence and spore germination associated transcriptome analysis will contribute to the understanding of the molecular mechanism of pathogenicity and spore germination of C. cassiicola .Results Firstly, we reported the draft genome sequences of a cucumber-sampled C. cassiicola isolate HGCC with high virulence. Although being conspecific, HGCC had distinct difference with a rubber-sampled isolate (CCP) and a human-sampled isolate (UM591) in genome sequences. The proportion of secreted proteins was 7.4% in HGCC. 28.6% of HGCC predicted genes were highly homologous to experimentally proven virulence-associated genes, which was close to that in CCP, UM591 and some plant fungal pathogens, but far more than 21.9% in Phaeosphaeria nodorum and 19.6% in Botrytis cinerea . Thousands of putative virulence-associated genes in various pathways or families were identified in HGCC. Secondly, a global view of the transcriptome of C. cassiicola spores during germination was evaluated using RNA sequencing (RNA-seq). A total of 3,288 differentially expressed genes (DEGs) were identified. The majority of KEGG annotated DEGs were involved in metabolism, genetic information processing, cellular processes, organismal system, human diseases and environmental information processing.Conclusions These results not only facilitated the exploration of the molecular pathogenic mechanism of C. cassiicola to cucumber and the understanding of molecular and cellular processes during spore germination, but also laid the foundation for the disease control.

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“…C. cassiicola spores germinated from one end or both ends at 25-30°C with > 90% relative humidity, and the germination rate in drops of water was the highest. C. cassiicola was able to invade cucumber leaves primarily through direct contact or via stomata [6]. C. cassiicola showed pathogenic and genetic variation among isolates sampled in different host plants, proving that the intraspecific strains of C. cassiicola showed high host specialization [2,7].…”

Section: Introductionmentioning

confidence: 98%

Genome sequence and spore germination-associated transcriptome analysis of Corynespora cassiicola from cucumber

Gao

Zeng

et al. 2020

BMC Microbiol

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Background: Corynespora cassiicola, as a necrotrophic phytopathogenic ascomycetous fungus, can infect hundreds of species of plants and rarely causes human diseases. This pathogen infects cucumber species and causes cucumber target spot, which has recently caused large cucumber yield losses in China. Genome sequence and spore germination-associated transcriptome analysis will contribute to the understanding of the molecular mechanism of pathogenicity and spore germination of C. cassiicola. Results: First, we reported the draft genome sequences of the cucumber-sampled C. cassiicola isolate HGCC with high virulence. Although conspecific, HGCC exhibited distinct genome sequence differences from a rubber treesampled isolate (CCP) and a human-sampled isolate (UM591). The proportion of secreted proteins was 7.2% in HGCC. A total of 28.9% (4232) of HGCC genes, 29.5% (4298) of CCP genes and 28.6% (4214) of UM591 genes were highly homologous to experimentally proven virulence-associated genes, respectively, which were not significantly different (P = 0.866) from the average (29.7%) of 10 other phytopathogenic fungi. Thousands of putative virulenceassociated genes in various pathways or families were identified in C. cassiicola. Second, a global view of the transcriptome of C. cassiicola spores during germination was evaluated using RNA sequencing (RNA-Seq). A total of 3288 differentially expressed genes (DEGs) were identified. The majority of KEGG-annotated DEGs were involved in metabolism, genetic information processing, cellular processes, the organismal system, human diseases and environmental information processing. Conclusions: These results facilitate the exploration of the molecular pathogenic mechanism of C. cassiicola in cucumbers and the understanding of molecular and cellular processes during spore germination.

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Histological observation of cucumber infected with Corynespora cassiicola

Cited by 13 publications

References 35 publications

Cell‐wall‐degrading enzymes produced by sesame leaf spot pathogen Corynespora cassiicola

Cell‐wall‐degrading enzymes produced by sesame leaf spot pathogen Corynespora cassiicola

Genome sequence and spore germination associated transcriptome analysis of Corynespora cassiicola from cucumber

Genome sequence and spore germination-associated transcriptome analysis of Corynespora cassiicola from cucumber

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