Histological Investigation of Flower Parts of Cucumber Infected by Didymella Bryoniae

Neergaard, Eigil de

doi:10.1080/07060668909501143

Cited by 20 publications

(15 citation statements)

References 17 publications

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“…In this study, GSB penetration pegs were observed early in infection that was attached to various pumpkin leaf. Attachment of penetration pegs was followed by pathogen spread and direct infection of both new and mature leaves, as observed in previous studies (De Neergaad, ; Van steekelenburg,). Previous studies have also shown that differential expression of fungal genes is observed mainly during two critical periods in the fungal life cycle: during host infection and vegetative body formation (Torres et al., ).…”

Section: Discussionsupporting

confidence: 71%

Identification and characteristics of Stagonosporopsis cucurbitacearum pathogenic factors influencing pumpkin seeding survival in north‐east China

Zhao

Zhang

et al. 2018

Journal of Phytopathology

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The parasitic pathogenic fungus Stagonosporopsis cucurbitacearum (Sc.) is the main cause of pumpkin gummy stem blight (GSB), one of the most devastating pumpkin crop diseases in north‐east China. In the present study, transcriptome analyses of the GSB‐susceptible strain commonly grown in that region, zq‐1, were performed to identify differentially expressed genes (DEGs) relevant to GSB infection. Using RNA‐seq of six fungal samples, we obtained 44.62 Gb of clean sequence data with a Q30 base percentage of 92.38% or higher. We carried out functional annotation by comparing sequences against NR, Swiss‐Prot, KEGG, COG, KOG, GO, and Pfam databases and obtained annotation results for 38,110 unigenes. From these unigenes, a total of 341 DEGs were identified and subjected to GO functional annotation and KEGG pathway analysis. Subsequently, 52 genes possessed putative pathogenic functions, with 34 genes assigned to molecular function (including hydrolase, protein kinase, phosphatase, cellulase, etc.), 15 genes assigned to biological process, and 3 genes assigned to mitogen‐activated protein kinase pathways, ABC transport proteins and cellular endocytosis. Identification of DEGs should enhance our understanding of pathogenic genes involved in pumpkin infection by Sc. and should facilitate the systematic investigation of pathogenic gene expression patterns and host–pathogen interactions.

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Section: Discussionsupporting

confidence: 71%

Identification and characteristics of Stagonosporopsis cucurbitacearum pathogenic factors influencing pumpkin seeding survival in north‐east China

Zhao

Zhang

et al. 2018

Journal of Phytopathology

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“…It was characteristic that the amount of external fruit rot increased under such conditions, similar to the increased incidence of lesions on the vegetative parts. This is in accordance with the histological similarities in pathogen development inthe host tissue in vegetative parts and in fruits after superficial penetration (= external fruit rot), whereas internal fruit rot, originating from the flower, has a quite different way of development, the pathogen causing far less necrosis in the initial stages after invasion of the host tissue (De Neergaard 1989a, 1989b.…”

Section: Discussionsupporting

confidence: 66%

“…Attack on the main stem -if not treated -usually kills the plant, Ascospores are airborne and thus responsible for the infection of the flowers. The pathogen invades the stigmatic tissue and follows the pollen transmitting tissue into the young fruit, where it later causes internal rot (de Neergaard, 1989a). Van Steekelenburg (1986) investigated the occurrence of internal fruit rot and concluded that a short period of flowering and a long style are factors which limit the incidence of internal rot.…”

Section: Introductionmentioning

confidence: 99%

Studies of Didymella bryoniae: The influence of nutrition and cultural practices on the occurrence of stem lesions and internal and external fruit rot on different cultivars of cucumber

Neergaard¹,

Haupt²,

Rasmussen³

1993

Netherlands Journal of Plant Pathology

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Cucumber plants of different cultivars grown under various levels of nutrient supply, climatic conditions and different pruning practices were inoculated with Didymella bryoniae (Auersw.) Rehm. Increased concentration of the nutrient solution in the rockwool slabs leading to a higher amount of dry matter content in the plant tissue resulted in a lower percentage of internal and external fruit rot in the cultivars Daleva and LD 290/82 (D~ehnfeldt), and in lower percentage of internal fruit rot in cv. Aminex, whereas the effect on cv. Dalibor was insignificant. Differences between cultivars were observed, cv. Dalibor showing the highest level of resistance against internal fruit rot and lowest incidence of nodal and internodal lesions.Separate factors appear to affect the establishment of the pathogen in the nodal tissue and its invasion of the internodal tissue. By raising the temperature to daytime level 3 h before sunrise, the risk of extended periods with high humidity was limited and the incidence of internal fruit rot was reduced. An increased incidence of nodal and internodal lesions and external fruit rot was observed at reduced pruning, leaving wilted leaves and shoots on the plants.

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“…Moreover, dimorphic variation in hyphal morphology, associated with latent infection and lesion expression under natural conditions, was shown for the first time. Hyphal dimorphism has been reported in gummy stem blight of cucumber caused by Didymella hryomae (NEERGAARD 1989(NEERGAARD a, 1989 and downy mildew of buckwheat caused by Peronospora ducometi (ZIMMER et al 1990). With L. maculans HAMMOND et al (1985) indicated that there is a shift from a biotrophic to a necrotrophic host-pathogen relationship during disease development.…”

Section: Discussionmentioning

confidence: 99%

Histopathology of Blackleg Development in Spring Rapeseed

Morrall

1993

Journal of Phytopathology

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Hyphae of Leptosphaeria maculans were observed in all types of above-ground tissue of two cultivars of Spring rapeseed plants exposed to natural infection. Large quantities of hyphae were distributed in the epidermis and cortex of the stem in symptomless plants at early growth stages, irrespective of resistance level of the cultivar. These results verified the extensive latent infection early in the season that had been detected by isolation in a previous study. Two types of hyphae occurred in plants: narrow hyphae (2.1 ,um diam.) were usually found in symptomless plants at early growth stages, whereas thick hyphae (5.7,um diam.) occurred in plants with symptoms as the late stages of growth. Resistance mechanisms were evidenced by the presence of heavily stained material and disintegration of the hyphae in cells of the resistant cultivar.

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Histological Investigation of Flower Parts of Cucumber Infected by Didymella Bryoniae

Cited by 20 publications

References 17 publications

Identification and characteristics of Stagonosporopsis cucurbitacearum pathogenic factors influencing pumpkin seeding survival in north‐east China

Identification and characteristics of Stagonosporopsis cucurbitacearum pathogenic factors influencing pumpkin seeding survival in north‐east China

Studies of Didymella bryoniae: The influence of nutrition and cultural practices on the occurrence of stem lesions and internal and external fruit rot on different cultivars of cucumber

Histopathology of Blackleg Development in Spring Rapeseed

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