Histopathology of Blackleg Development in Spring Rapeseed

Xi, K.; Morrall, R. A. A.

doi:10.1111/j.1439-0434.1993.tb01322.x

Cited by 6 publications

(2 citation statements)

References 11 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The growth pattern in the stem, whereby hyphae grew intercellularly in the cortex at the infection front, behind which growth was intracellular and hyphae proliferated into the vascular tissue and pith, is consistent with that observed by Xi & Morrall (1993). Leptosphaeria maculans grows within xylem vessels in the petiole (Hammond et al ., 1985; Eckert et al ., 2005); however, the route of hyphal growth at the petiole–stem junction and entry into xylem vessels in the stem is unclear.…”

Section: Discussionsupporting

confidence: 88%

“…Hammond & Lewis (1987) observed that, even in susceptible cultivars, hyphae advanced from the petiole into the stem within xylem vessels but emerged and grew in the cortex and pith but not in the vascular tissue once inside the stem. Other studies have reported hyphae in xylem vessels in the stems of plants inoculated at the stem–petiole junction (Sexton & Howlett, 2001) and in plants infected naturally in the field (Xi & Morrall, 1993). Hyphae may follow xylem vessels from the petiole into the stem; however, these junctions are anatomically complicated and vessels are generally discontinuous (Esau, 1977).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Pathways of infection of Brassica napus roots by Leptosphaeria maculans

et al. 2007

View full text Add to dashboard Cite

Summary• Infection of Brassica napus cotyledons and leaves by germinating ascospores of Leptosphaeria maculans leads to production of leaf lesions followed by stem cankers (blackleg). Leptosphaeria maculans also causes root rot but the pathway of infection has not been described.• An L. maculans isolate expressing green fluorescent protein (GFP) was applied to the petiole of B. napus plants. Hyphal growth was followed by fluorescence microscopy and by culturing of sections of plant tissue on growth media. Leptosphaeria maculans grew within stem and hypocotyl tissue during the vegetative stages of plant growth, and proliferated into the roots within xylem vessels at the onset of flowering. Hyphae grew in all tissues in the stem and hypocotyl, but were restricted mainly to xylem tissue in the root.• Leptosphaeria maculans also infected intact roots when inoculum was applied directly to them and hyphae entered at sites of lateral root emergence. Hyphal entry may occur at other sites but the mechanism is uncertain as penetration structures were not observed.• Infection of B. napus roots by L. maculans can occur via above-and belowground sources of inoculum, but the relative importance of the infection pathways under field conditions is unknown.

show abstract

Section: Discussionsupporting

confidence: 88%