Histological fixation by microwave heating.

Mayers, Cathy

doi:10.1136/jcp.23.3.273

Cited by 154 publications

(59 citation statements)

References 2 publications

(2 reference statements)

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“…After fixation by microwave the tissues became firm and pale in colour as also noted by Mayer CP [13] and Leong ASY et al, [17] on macroscopic examination.…”

Section: Discussionsupporting

confidence: 67%

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Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation

Tripathi

Bansal

Gupta

et al. 2013

JCDR

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Introduction: Conventional formalin-fixed, paraffin-embedded tissue provides superior cellular morphology and long-term storage. Problems with formalin fixation comprise delay of fixation and variations in the duration of fixation. Microwave assisted tissue fixation removes the use of noxious and potentially toxic formalin that decreases the turnaround time and creates a personnel friendly workflow.

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“…After fixation by microwave the tissues became firm and pale in colour as also noted by Mayer CP [13] and Leong ASY et al, [17] on macroscopic examination.…”

Section: Discussionsupporting

confidence: 67%

“…In the present study microwave irradiation has been used only for fixation of tissues like Mayer CP [13] and Pitol DL et al, [14].…”

Section: Discussionmentioning

confidence: 97%

Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation

Tripathi

Bansal

Gupta

et al. 2013

JCDR

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“…Since the pioneering work of Mayers (1970), MWI has been utilized to accelerate biological specimen processing for light and electron microscopy. Because it raises the temperature and greatly increases molecu- (Boon et al 1990), MWI enables chemicals such as fixatives to diffuse rapidly into cells and tissues.…”

Section: Resultsmentioning

confidence: 99%

Application of Microwave Technology to the Processing and Immunolabeling of Plastic-embedded and Cryosections

Rangell¹,

Keller²

2000

J Histochem Cytochem.

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S U M M A R YWe have adapted existing microwave irradiation (MWI) protocols and applied them to the processing and immunoelectron microscopy of both plastic-embedded and frozen sections. Rat livers were fixed by rapid MW irradiation in a mild fixation solution. Fixed liver tissue was either cryosectioned or dehydrated and embedded in Spurr's, Unicryl, or LR White resin. Frozen sections and sections of acrylic-embedded tissue were immunolabeled in the MW oven with an anti-catalase antibody, followed by gold labeling. Controls were processed conventionally at room temperature (RT). The use of MWI greatly shortened the fixation, processing, and immunolabeling times without compromising the quality of ultrastructural preservation and the specificity of labeling. The higher immunogold labeling intensity was achieved after a 15-min incubation of primary antibody and gold markers under discontinued MWI at 37C. Quantification of the immunolabeling for catalase indicated a density increase of up to fourfold in the sections immunolabeled in the MW oven over that of samples immunolabeled at RT. These studies define the general conditions of fixation and immunolabeling for both acrylic resin-embedded material and frozen sections.

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“…During the past decade, various antigen retrieval methods including microwave irradiation and autoclave treatment have been introduced to the immunohistochemical field and their beneficial effects were repeatedly documented [10,12]. Shi et al reported a microwave oven heating technique for retrieval of a variety of cell surface, cytoplasmic, and nuclear antigens [16].…”

Section: Introductionmentioning

confidence: 99%