Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation

Tripathi, Meenakshi; Bansal, Rani; Gupta, Mamta; Bharat, Vinay

doi:10.7860/jcdr/2013/6233.3754

Cited by 5 publications

(5 citation statements)

References 26 publications

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“…[ 17 ] and Tripathi et al . [ 18 ] found the RBCs to be intact without any lysis in microwave assisted tissue fixation methods. Whereas Hopwood et al .,[ 19 ] Mayers,[ 20 ] Leong et al .,[ 16 ] Bernard[ 21 ] found RBCs to be lysed after microwave processing.…”

Section: Discussionmentioning

confidence: 99%

Microwave-Based Rapid Tissue Processing Technique: A Novel Aid in Histopathologic Laboratory

Mishra¹,

Bandyopadhyay²,

Kumar³

et al. 2021

Journal of Pharmacy and Bioallied Sciences

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Introduction: Conventional tissue processing takes a lot of time, but microwave processing method though rapid, its quality of tissue texture and staining properties is still questionable. Therefore, we conducted this study to find out the reliability of histological sections in the microwave method. Aim and Objectives: The aim is to assess whether microwave tissue processing, which is a rapid technique, is comparable to conventional tissue processing in terms of quality and reliability of histological sections. Materials and Methods: A total of 80 formalin-fixed tissue samples were taken and were divided into two pieces. One subjected to conventional tissue processing and the other subjected to microwave tissue processing. The slides were coded and evaluated by three independent observers using various parameters. All the data were subjected to Chi-square test. Results: Both the process did not differ in their efficiency in overall nuclear details, overall cytoplasmic detail, occurrence of artifact and tissue architecture, nuclear staining, cytoplasmic staining, and red blood cell staining. Although the other observers did not find any statistical significance, according to observer 3, conventional processing proved to have marginally significantly better epithelial connective tissue interface than microwave processing ( P = 0.047). Conclusion: The microwave method of tissue processing is an economical procedure which reduces the obnoxious chemicals as well as the turnaround time.

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Section: Discussionmentioning

confidence: 99%

Microwave-Based Rapid Tissue Processing Technique: A Novel Aid in Histopathologic Laboratory

Mishra¹,

Bandyopadhyay²,

Kumar³

et al. 2021

Journal of Pharmacy and Bioallied Sciences

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“…Tissue fixations consider the keystone for preserving biological samples, and the fixative is the tools that used to get rigid tissue fixation be completed; fixation and fixative are used mainly to stop tissue decomposition and decays that are either caused by the action of saprophytic bacteria or by the act of proteolytic enzymes in the cell (23). Fixation, in our opinion, is considered the critical stage in tissue processing since it stops postmortem changes and prevents further biochemical activity in the cell.…”

Section: Tissue Fixationmentioning

confidence: 99%

“…Fixation, in our opinion, is considered the critical stage in tissue processing since it stops postmortem changes and prevents further biochemical activity in the cell. In addition, the next steps rely on it for being accurate and correct (24). Formaldehyde is considered the gold solution in the fixation process.…”

Section: Tissue Fixationmentioning

confidence: 99%

Standard techniques for formalin-fixed paraffin-embedded tissue: A Pathologist’s perspective

Al-Sabaawy¹,

Rahawi²,

Al-Mahmood³

2021

IJVS

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Histopathology considered as an essential keystone to understanding diseases on a cellular level, without examining affected tissues and cells; will lack the accurate diagnosis. The most common protocol that used worldwide for histopathology is termed as formalinfixed paraffin-embedded techniques; this protocol is the oldest protocol and still used, this technique not invented once, but it is started with a simple process of tissue fixation then undergo several successful steps as it's known today. This protocol depends on formalin to induce tissue fixation and preservation simultaneously, followed by a series of steps to facilitate infiltration of hot paraffin wax in the cells to adjust the density of tissue similar to that of paraffin wax to support tissue during sectioning process at few micrometers of thickness. Many protocols have been invented and modified subsequently to increase the accuracy of tissue processing and facilitate their study under light microscopy. Additionally, the formalin-fixed and paraffin-embedded samples can be considered the most durable process till now. In this review, we are trying to view all fundamental aspects of classical tissue processing that was depended since 1850s, starting from tissue examination, sampling, accessory, labelling, fixation, dehydration, clearance, infiltration, and embedding with hot paraffin wax. Additionally, explain the routine sectioning and staining with classical Hari's hematoxylin and alcoholic eosin staining protocol.

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“…Grading was done by adding the score of each parameter. 5-10 = Poor; 11-15 = Fair; 16-20 = Good; 21-25 = Excellent; >25 = Outstanding [6].…”

Section: Ethical Consideration and Permission: Institutional Review Boamentioning

confidence: 99%

A study on qualitative comparison between cryostat and conventional technique for histopathology diagnosis

Zinzala

Goswami

et al. 2020

Trop J Pathol Microbiol

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Introduction: Intraoperative consultation by frozen section technique is an invaluable tool for immediate diagnosis. The correlation of intraoperative frozen section diagnosis with final diagnosis on permanent section is an integral part of quality assurance in surgical pathology. Its accuracy and limitations vary with different anatomical sites. Aims & Objective: Qualitative morphological comparison between frozen section and routine formalin fixed paraffin embedded sections in different tissues, to assess the accuracy of frozen section, to detect the number and type of discrepancies and to assess the causes for discrepancies. Material and Method: The present study retrospectively reviewed frozen sections performed in the pathology department, Sirt hospital, government medical college, Bhavnagar during a period of 2 year. Diagnostic accuracy of frozen section and its morphological quality and reliability in comparison to histopathology was evaluated by 2 pathologists in a blinded fashion for the following parameters: cellular outline, nuclear and cytoplasmic features, staining pattern and overall morphology. The results were compared to the routine formalin fixed paraffin sections to evaluate diagnostic accuracy. Discordant cases were reassessed to find the reasons for discrepancy. Result: Diagnostic accuracy of frozen section was 95.1% with false negative case are 4.8% and no false positive case. Statistical analysis showed that most common indications of frozen section in the present study was presence/typing of neoplasm (61.2%) followed by assessment of margin (35.6%) and assessment of nodal status (3.2%). It was observed that nuclear details, cellular outline and overall morphological quality of frozen section was slightly inferior to that of routine histopathology section, however, staining and cytoplasmic details were comparable. The discrepancies were mainly due to technical artefacts (9.7%), sampling error (3.2%) interpretation error (1.6%), and partly due to lack of interdepartmental communication. Conclusion: Frozen section diagnosis is very useful and highly accurate procedure. Gross inspection, sampling by pathologist, frozen complemented with cytological and histological review and intimal cooperation with surgeon can avoid certain limitations and provide rapid, reliable, cost effective information necessary for optimum patient care.

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Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation

Cited by 5 publications

References 26 publications

Microwave-Based Rapid Tissue Processing Technique: A Novel Aid in Histopathologic Laboratory

Microwave-Based Rapid Tissue Processing Technique: A Novel Aid in Histopathologic Laboratory

Standard techniques for formalin-fixed paraffin-embedded tissue: A Pathologist’s perspective

A study on qualitative comparison between cryostat and conventional technique for histopathology diagnosis

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