Histological evaluation of the possible role of Na+/ H+ entiporter and anion exchanger in endochondral ossification activities of secondary bone healing in rats

Ibrahim, S. M.; Handool, Kareem Obayes; Abdul, Abubakar Adamu; Abu, Jalila; Yusof, Sabri; Ibrahimmi, Mehdi; Yusof, Loqman Mohamad

doi:10.33899/ijvs.2019.125832.1165

Cited by 7 publications

(7 citation statements)

References 27 publications

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“…These variations in the time of developing of ossification centres of tarsal bones that were documented in our data, could be due to the genetic background that control every cell activity during endochondral ossification (24). Another study mentioned that embryonic bone development including the geometric properties like medullary area and thickness and even the mechanical characters such as power to fracture and ultimate load is a completely and genetically programmed event that is modified by epigenetic factors (25).…”

Section: Discussionsupporting

confidence: 60%

A study of primary ossification centers in the hind limbs of Awasi sheep fetuses by double stains method and radiography.

Salih¹,

Ahmed²

2022

IJVS

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Forty Local Awasi sheep fetuses were collected from several areas in Nineveh Governorate, further than local slaughter house. Their estimated ages calculated depending on crown-rump length with the aid of Richardson formula. The study aimed to detect the site and the time of appearance of centers of ossification in the bones of hind limb of Awasi sheep by using modified method of double stains to demonstrate cartilage with bone, or by using radiography. The first ossification centers appeared in the hind limb bones was the primary center of the tibia and femur, metatarsus with ilium then ischium at 45, 46, 48, 50, 52 days of fetal age respectively. The ossification centers of pes phalanges appeared in the following sequence; distal phalanx, followed by the proximal phalanx and end by middle phalanx, at 58, 60, 64 days respectively. Ossification centers of tarsal bones began to appear in calcaneus at 64 days, talus at 77 days, central and 4th tarsal bones at 96 days, followed by centers of 3rd and 2nd tarsal bones at 100 days while, the center of 1st tarsal bone appeared lastly at 105 days. Femur, tibia and metatarsus studied statistically by follow up the relative increase of their total lengths with ossified portion length of their diaphyses. The very important finding of our work is that the maximum relative increment in the length of the long bones and their ossified diaphysis is happening within the 7th to 8th weeks of gestation making this time to be considered as the most critical period for bone development especially long bones so it is essential to avoid giving any medication or therapy during that time which could affect or even retard this vital process.

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Section: Discussionsupporting

confidence: 60%

A study of primary ossification centers in the hind limbs of Awasi sheep fetuses by double stains method and radiography.

Salih¹,

Ahmed²

2022

IJVS

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“…Uterus biopsy was fixed in neutral buffered 10 % formalin and paraffin embedded section were stained with hematoxylin and eosin, then microscopically examined for the detection of tachyzoite and tissue cysts of T. gondii (11,12).…”

Section: Histological Methodsmentioning

confidence: 99%

Comparison the efficiency of different techniques for the diagnosis of Toxoplasma gondii infection in slaughtered ewes

Aghwan¹,

Al-Bakri²,

Albaqqal³

2021

IJVS

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Toxoplasma gondii is one of the most common parasitic infections of human and other warm-blooded animals causes toxoplasmosis. In the present study a total of 50 uterus samples collected from slaughtered ewes were investigated for detection of T. gondii. Several techniques have been used to diagnose the infection with this parasite. Firstly, the impression smears staining methods used for the all samples using giemsa stain. Secondly, uses of direct fluorescence technique by acridine orange method for staining the impression smears of the uteri. As well as the histological section technique was used to determine the developmental growth stages of the parasite of all uterus samples and finally the serological method by latex agglutination test was used for the detection of antibodies of parasite. The results showed that detection of T. gondii using these four methods was 100, 80, 80 and 50%, respectively. It was concluded that the impression smears of the uterus staining with Giemsa stain was more readily, effectively and efficiently, followed by the direct immunofluorescence technique and histological section stained with hematoxylin and eosin stain technique, and finally the serological method.

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“…Parts of diaphragm muscles from each sample were fixed in 10% neutral buffer formalin at least 48 hours. The fixed muscles samples were trimmed into 0.5 cm 3 thick section dehydrated with serial dilutions of ethanol and xylene, then embedded in paraffin block for sectioned to 3-5 mm, then stained with hematoxylin and eosin (H&E) and Masson's trichrome examined for detection of microcysts with light microscope (10)(11)(12).…”

Section: Histopathological Examinationmentioning

confidence: 99%

Pathological and molecular study of ovine diaphragms naturally infected by Sarcosystis spp.

Mostafa¹,

Alhayali²,

Suleiman³

2021

IJVS

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Sarcosystis spp., has a close relationship with muscles due to its unique localization within skeletal muscle in humans and the animals it infects, as the chronic condition of the disease causes significant economic losses, especially in terms of meat production as a result of the formation of cysts, whether macroscopic or microscopic, in their muscle fibers. Sarcosystis tenella and Sarcosystis arieticanis are the most important pathogenic cysts forming in sheep. In this study, 50 samples of diaphragm muscles of sheep slaughtered in the butchers' shops and the Mosul abattoir were examined grossly, histologically, and using PCR technique as a diagnostic tool to identify or diagnose the causative and responsible species of these changes. The diaphragm samples appeared white and pale on the macroscopic examination, while the tissue lesions were characterized by the presence of Sarcosystis in different numbers and sizes among the muscle fibers, which led to the occurrence of zinker necrosis and intense infiltration of inflammatory cells, especially eosinophil, monocyte, macrophage and giant cells, and also oedema and proliferation of fibroblast. With the formation of fibrous tissue whose intensity was inferred (mild, medium and intense) by using the masson's trichrome stain. The results of the molecular analysis using the nested PCR technique indicated that these diagnosed microscopic cysts belong to Sarcosystis tenella with a reaction product of 800bp and 500bp.

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Histological evaluation of the possible role of Na+/ H+ entiporter and anion exchanger in endochondral ossification activities of secondary bone healing in rats

Cited by 7 publications

References 27 publications

A study of primary ossification centers in the hind limbs of Awasi sheep fetuses by double stains method and radiography.

A study of primary ossification centers in the hind limbs of Awasi sheep fetuses by double stains method and radiography.

Comparison the efficiency of different techniques for the diagnosis of Toxoplasma gondii infection in slaughtered ewes

Pathological and molecular study of ovine diaphragms naturally infected by Sarcosystis spp.

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