Comparison the efficiency of different techniques for the diagnosis of Toxoplasma gondii infection in slaughtered ewes

Abstract

Toxoplasma gondii is one of the most common parasitic infections of human and other warm-blooded animals causes toxoplasmosis. In the present study a total of 50 uterus samples collected from slaughtered ewes were investigated for detection of T. gondii. Several techniques have been used to diagnose the infection with this parasite. Firstly, the impression smears staining methods used for the all samples using giemsa stain. Secondly, uses of direct fluorescence technique by acridine orange method for staining the impression smears of the uteri. As well as the histological section technique was used to determine the developmental growth stages of the parasite of all uterus samples and finally the serological method by latex agglutination test was used for the detection of antibodies of parasite. The results showed that detection of T. gondii using these four methods was 100, 80, 80 and 50%, respectively. It was concluded that the impression smears of the uterus staining with Giemsa stain was more readily, effectively and efficiently, followed by the direct immunofluorescence technique and histological section stained with hematoxylin and eosin stain technique, and finally the serological method.

Toxoplasmosis, a zoonotic infection; A critical and updated analysis: A Review Article

“…As the use of the medication throughout pregnancy resulted in improved clinical results. In summary, many zoonotic diseases exist (43)(44)(45)(46)(47); Toxoplasmosis still has many layers still to be unraveled.…”

Detection of Cryptosporidium parvum in calf feces using microscopical, serological, and molecular methods

“…Indirect serological methods, like latex agglutination test (LAT), modified agglutination test (MAT), ELISA enzyme-linked immunosorbent assay which, depend on coproantigen detection. Coproantigen detection assays are rapid and useful for screening large numbers of specimens simultaneously but do not provide details concerning the species of apicomplexan protozoal included Cryptosporidium detected (10,11). Molecular methods, e. g. conventional polymerase chain reaction (PCR), are used to detect parasite DNA but needed amplification before PCR test to overcome eliminate or inactivation inhibitors (12,13).…”

Routine red bone marrow smear in local breed claves at Basrah, Iraq

“…The value of red bone marrow elements distinguishes of disease status from peripheral blood counts such as leukemia, congenital hematopoietic defect, and cytological responses for bone marrow (6,7). Most endemic diseases in Iraq such as anaplasmosis, babesiosis and theileriosis (8,9), and other disease states associated with anemia (10,11), may also be related to a defect in erythropoiesis and myelopoiesis (12,13), which can be characterized by a reduction in the levels of erythrocytes count, and of their other parameters in the blood periphery also dysplasia in one or more hematologic cell lines in the bone marrow (14,15). Authors assure that blood-borne diseases accompanied by diminished granulopoiesis and megakaryocytopoiesis in the bone marrow in the infected animal also the erythroid suppression, lymphocytic, monocytic, and macrophage hyperplasia are also found (3,16,17).…”