Histological evaluation of the healing process of autografted mandibular bone defects in rats under treatment with zoledronate

Zandi, Mohammad; Dehghan, Arash; Talimkhani, Ideh; Rezaeian, Leila; Mezerji, Naser Mohammad Gholi

doi:10.1016/j.jcms.2019.08.003

Cited by 3 publications

(2 citation statements)

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“…Histology has been widely used to assess bone healing at the microscale throughout the entire process (Manjubala et al, 2009;Mora-Macías et al, 2017) to identify the type of defect (Epari et al, 2006;Zandi et al, 2019), to evaluate fixation efficiency (Claes et al, 2008;Meeson et al, 2019) classify the mineralised and unmineralised tissue formation in the healing stages (Epari et al, 2006;Manjubala et al, 2009). The mineralisation of the fracture over time provides information on the effect of fixator stiffness (Zandi et al, 2019), on the use of biomaterials and pharmaceuticals to enhance fracture healing. This investigates only selected two-dimensional (2D) regions of the tissue.…”

Section: Introductionmentioning

confidence: 99%

Full‐field strain of regenerated bone tissue in a femoral fracture model

Karali

Kao

Meeson

et al. 2020

Journal of Microscopy

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The mechanical behaviour of regenerated bone tissue during fracture healing is key in determining its ability to withstand physiological loads. However, the strain distribution in the newly formed tissue and how this influences the way a fracture heals it is still unclear. X-ray Computed Tomography (XCT) has been extensively used to assess the progress of mineralised tissues in regeneration and when combined with in situ mechanics and digital volume correlation (DVC) has been proven a powerful tool to understand the mechanical behaviour and full-field three-dimensional (3D) strain distribution in bone. The purpose of this study is therefore to use in situ XCT mechanics and DVC to investigate the strain distribution and load-bearing capacity in a regenerating fracture in the diaphyseal bone, using a rodent femoral fracture model stabilised by external fixation. Rat femurs with 1 mm and 2 mm osteotomy gaps were tested under in situ XCT stepwise compression in the apparent elastic region. High strain was present in the newly formed bone (ε p1 and ε p3 reaching 29 000 µε and -43 000 µε, respectively), with a wide variation and inhomogeneity of the 3D strain distribution in the regenerating tissues of the fracture gap, which is directly related to the presence of unmineralised tissue observed in histological images. The outcomes of this study will contribute in understanding natural regenerative ability of bone and its mechanical behaviour under loading.

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Section: Introductionmentioning

confidence: 99%

Full‐field strain of regenerated bone tissue in a femoral fracture model

Karali

Kao

Meeson

et al. 2020

Journal of Microscopy

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“…These drugs raise the bone density and decrease the danger of fracture in osteoporotic individuals. In addition, calcium metabolic diseases as hypercalcemia of malignancy, Paget’s disease, multiple myeloma are treated by these agents [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Bisphosphonate’s effect on the tongue in adult male albino rats and the possible protective role of rutin: light and scanning electron microscopic study

El-ghazouly,

Yassien

2024

Anat Cell Biol

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Alendronate sodium (ALS) is a nitrogen-containing bisphosphonate used for the treatment of different bone disorders. However, its adverse effect on oral soft tissue has been detected. Rutin (RUT) is natural flavonoid with antioxidant and anti-inflammatory properties. This work aimed to investigate the possible effect of ALS on the tongue of adult male albino rats and to evaluate the possible protective role of RUT. Forty adult male albino rats were equally divided into four groups: group I (control), group II (RUT): Received RUT 50 mg/kg, group III (ALS): Received ALS 1 mg/kg, group IV (ALS+RUT): Received ALS and RUT with the same doses as pervious groups. The drugs were given once daily for 5 weeks. Tongue specimens were taken and processed for light and scanning electron microscopic inspection. ALS treated group revealed structural changes in the tongue in the form of decrease in the height of the filiform papillae with blunt ends, marked atrophy in some papillae with areas of focal loss, loss of some epithelial cells, pyknotic nuclei and cytoplasmic vacuoles in some epithelial cells. The lamina propria showed inflammatory cellular infiltration with congested blood vessels. Statistically, there were highly significant decrease in the number of proliferating cell nuclear antigen immunopositive cells, area percentage of Bcl-2 immunoexpression and highly significant increase in the collagen content compared to control group. Administration of RUT with ALS minimizes these changes. RUT protected the rat tongue against the histological and immunohistochemical changes induced by ALS through its antioxidant and anti-inflammatory properties.

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