Histological Characterization of Sacco's Concentrated Growth Factors Membrane

Abstract

SUMMARY:Along with the emerging needs of the dental patients, numerous techniques for oral tissue stimulation and regeneration were developed to be employed in the modern implant rehabilitation therapies. The Concentrated Growth Factors (CGF) are a relatively new therapeutic presidium that can be used for this purpose, enhancing the regenerative potential property of blood cells. Although literature presents numerous studies evaluating the CGF for their clinical uses and efficacy, data regarding their biological characteristics are very few. The present study evaluates and describes the CGF structural morphology by means of classical histological methods, using haematoxilin-eosin and azan mallory stains. A three layers organization with a fibrin complex network was noted, with blood corpuscular elements entrapped, especially in the most external layer. These descriptions enrich the knowledge about this new type of membrane, showing the bio-morphological side of the regenerative techniques. These findings will be useful in clinical practice for the choice of the most suitable technique in each implant rehabilitation.

Maintenance of Alveolar Ridge Dimensions Utilizing an Extracted Tooth Dentin Particulate Autograft and Platelet-Rich fibrin: A Retrospective Radiographic Cone-Beam Computed Tomography Study

“…This feature may be beneficial for volume maintenance since at the remodeling stage dentin graft is resorbed slowly. As a supplement of bone graft material, the use of platelet concentrates such as platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factors (CGF), which regulate inflammation and angiogenesis, could be beneficial [14]. These biological additives were identified as producers of growth factors that promote the wound healing and regenerative process [15,16].…”

Release of VEGF from Dental Implant Surface (IML® Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future Expectations

“…CGF is constituted by a fibrin network that includes many cellular components, such as stem cells and growth factors [8,15]. The CGF exerted its effects through the degranulation of the platelet granules, which contained various growth factors that are considered important in the initial phase of wound healing.…”

“…Platelets, in particular, contain biologically active proteins at high concentrations and support cell healing, growth, and morphogenesis [6]. In addition to platelets, CGF contains fibroblasts, leukocytes, and endothelial cells for angiogenesis and tissue remodeling; and provides a matrix for cell migration [7,8]. CGF is a fibrin biomaterial rich in the growth factors obtained by centrifugation of venous blood, at alternating speeds, as set on the Silfradent device [8].…”

Evaluation of Effectiveness of Concentrated Growth Factors on Osseointegration Around Immediate Dental Implant

“…The immediately placed implants have many advantages including reduction of number of surgeries performed, preservation of hard and soft supporting tissues,preventing of bone loss, decreased expense.however, immediate implant placement in mandibular molar sites have been a subject of debate due to the difficulty in achieving primary stability, poor bone quality, possibility of loos of the interseptal/ interradicular bone during the extraction in addition to the increase in dimension of extracted socket. (10) Concentrated Growth Factor (CGF)membrane +(CGF)sticky bone was prepared from patient own blood before insertion of the implant in the socket. The preparation of CGF was completely under aseptic condition, mixture of CGF+ bone graft was done in sterilize container and are placed around the placement implant this direct preparation helped with blood clot after fixed implant with primary stability to rapid healing, the(CGF)sticky bone fill the space around the implant and the bony walls of the socket which help in rapid bone formation and the granules act as nexus for starting osseointegration.…”