Histological aspects and structural characteristics of the testes of Dendropsophus minutus (Anura, Hylidae)

Santos, Lia Raquel de Souza; Oliveira, Classius de

doi:10.1016/j.micron.2008.03.006

Cited by 21 publications

(13 citation statements)

References 16 publications

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“…Our results corroborate the results of Santos and Oliveira (2008), and Leite et al (2015), who pointed out that, in general, the spermatogenesis process in anurans is cystic, with the organization of germ cells always in the same stage of differentiation. However, the organization of these cysts may vary among different families of anurans (proportion of germ cells present and/or absent) and especially according to the environment where these animals occur, as found by Ferreira et al (2009) for Dendropsophus nanus (Boulenger, 1889), Pseudis limellum (Cope, 1862) and Pseudis paradoxa (Linnaeus, 1758) of Hylidae and the Leptodactylus chaquensis Cei, 1950 andLeptodactylus podicipinus (Cope, 1862), species of Leptodactilidae from the Pantanal region.…”

Section: Discussionsupporting

confidence: 92%

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Influence of rainfall and temperature on the spermatogenesis of Leptodactylus macrosternum (Anura: Leptodactylidae)

Chaves¹,

Moura²,

Tenório³

et al. 2017

Zoologia

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In the semi-arid environment, the reproductive success of anurans depends on adaptations in their life cycle, which synchronizes with ideal environmental conditions to maximize the number of offspring. In this study changes in the histological and morphometric aspects of the testes of Leptodactylus macrosternum Miranda-Ribeiro, 1926 are characterized, to evaluate the influence of rainfall and temperature on them. Specimens were collected at Horto Florestal Olho d'Água da Bica-HFOB (06°49'20"S, 36°15'85"W) area, municipality of Cuité, state of Paraíba, Brazil. Search for specimens was active, happened at night, and amounted to 15 days from January to December 2013. The densities of spermatids (primary and secondary), sperm and area of the seminiferous locules of the testes were registered. The influence of climate variables (rainfall and temperature) on the density of primary and secondary spermatids, sperm and locular area were verified using Simple Linear Regression. Primary spermatids had the lowest density in July (57.90 ± 51.54 mm 2), with a peak in November (300.32 ± 117.35 mm 2); secondary spermatids had the lowest density in December (287.87 ± 79.05 mm 2), with a peak in May (135,727.00 ± 301.13 mm 2); sperm was in the lowest density in July (237.37 ± 121.10 mm 2), with a peak in June (2,270.45 ± 602.62 mm 2) and the locular area had the lowest density in December (40,292.9 ± 8,174.20 µm 2) and highest density in June (338,875.01 ± 2,262.10 µm 2). A notable decrease in sperm density was evident between June and July. That, associated with the observation of a larger locular area in June, allowed us to identify as June as the peak of spermatogenesis and the following month as the most potentially reproductive. The density of secondary spermatids(r = 0.02), sperm (r = 0.21) and locular area (r = 0.01) showed dependency on rainfall whereas only sperm (r = 0.09) showed dependency on temperature. Therefore, we can state that the reproductive cycle of L. macrosternum is potentially continuous, with a reproductive peak in July.

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Section: Discussionsupporting

confidence: 92%

“…In tropical regions of seasonal climate, the reproductive activity of anurans is associated with the rainy season (Santos and Oliveira 2008). This is consistent with the lack of records for the dry period, between January and April, when humidity is very low and rainfall rates fall in the Curimatau macroregion of the state of Paraíba.…”

Section: Discussionsupporting

confidence: 85%

Influence of rainfall and temperature on the spermatogenesis of Leptodactylus macrosternum (Anura: Leptodactylidae)

Chaves¹,

Moura²,

Tenório³

et al. 2017

Zoologia

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“…In relation, to the next stage of development that includes secondary spermatogonia, a phase of intense cell division that remains until the formation of early spermatids (Lofts, 1974;Rastogi et al, 1988) it was possible to verify great similarity with the descriptions of other species including Pseudis limellum (Ferreira et al, 2008). These are generally more basophilic cells than primary spermatogonia (Jamieson, 2003), which contrasts with what was observed in R. arenarum (Burgos & Fawcett, 1956;Cavicchia & Moviglia, 1983) and D. minutus (De Souza-Santos & De Oliveira, 2008). At the same time, secondary spermatogonia were one of the scarcest cell types in the sections analyzed and even in the testes of some species it has not been found (Asenjo et al, 2011;Iturriaga et al, 2012).…”

Section: Discussionmentioning

confidence: 77%

Histology and structure of the testicles in three species of Atelopus frogs (Anura: Bufonidae) endemic to the Sierra Nevada de Santa Marta, Colombia

2021

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Introduction: Testicular histology constitutes one of the least explored aspects in frogs of the genus Atelopus. This taxonomic group shows an alarming population decline; therefore, its reproductive biology is one of the greatest topics of interest for its conservation. Objective: To describe the testicular morphology and the spermatogenetic lineage cells in adult males of Atelopus laetissimus, Atelopus nahumae, and Atelopus carrikeri in the Sierra Nevada de Santa Marta, Colombia. Methods: During June – July 2017 and 2018, sampling was conducted in the localities of San Lorenzo and Páramo Cebolletas, Sierra Nevada de Santa Marta (SNSM), to collect 15 adult males, 5 per species. Testes samples were fixed in Bouin to be processed by the standard paraffin-embedding technique. Histological sections (3 mμ) were stained with Hematoxylin-eosin and Mallory-Heidenhain-Azan-Gomori's. For the description and photographic register of the germ cells, the photonic microscopy technique was used with the differential interference contrast system. Results: The testes are oval organs, compact, light yellow color, and with little vascularization. Externally, they are surrounded by a thin albuginea tunic constituted by regular dense connective tissue. Inside this layer, they are composed of numerous seminiferous tubules of hexagonal contour, in which germ cell cysts are distinguished at different stages of spermatogenesis (spermatogonia I and II, spermatocyte I and II, and early and late spermatids) and spermiogenesis (spermatozoa in fascicles and free spermatozoa). Separating the seminiferous structures is the interstitial tissue in which Leydig cells and blood vessels stand out. Additionally, in the cranial part of the testis, the Bidder's organ was found, formed by two distinguishable regions, the cortex and the medulla. In the cortex, there are previtellogénic oocytes of different sizes surrounded by a monolayer of flat follicular cells. For its part, the medullary region is the connective tissue that nourishes the oocytes and is constituted by blood capillaries. Conclusions: The gonads of the three species analyzed present a cystic cellular organization similar to other anurans, where all stages of spermatogenesis and spermiogenesis were identified, possibly indicating a continuous reproductive activity. Likewise, the Bidder’s organ is reported for the first time in the three Atelopus species, which allows suggesting a possible sexual reversion in case of a population decrease of females as a reproductive strategy.

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“…We embedded the fixed testes in plastic (Technovit 7100), and prepared 3 μm thin longitudinal sections with a microtome (Leica JUNG RM 2065). The thin sections were stained with 1% toluidine blue and borax (de Souza Santos and de Oliveira ).…”

Section: Methodsmentioning

confidence: 99%

“…Differentiation stages of sperm cells were determined as described in de Oliveira et al () and de Souza Santos and de Oliveira (). We report the results on the last three developmental stages: elongated spermatids, sperm bundles, and spermatozoids, because earlier developmental stages did not show any differences between hybrids and pure‐species animals.…”

Section: Methodsmentioning

confidence: 99%

Failed sperm development as a reproductive isolating barrier between species

Wunsch

Pfennig

2013

Evolution and Development

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SUMMARYHybrid male sterility is a common reproductive isolating barrier between species. Yet, little is known about the actual developmental causes of this phenomenon, especially in naturally hybridizing species. We sought to evaluate the developmental causes of hybrid male sterility, using spadefoot toads as our study system. Plains spadefoot toads (S. bombifrons) and Mexican spadefoot toads (S. multiplicata) hybridize where they co-occur in the southwestern USA. Hybrids are viable, but hybrid males suffer reduced fertility. We compared testes size and developmental stages of sperm cell maturation between hybrid males and males of each species. We found that testes of hybrid males did not differ in mean size from pure-species males. However, hybrids showed a greater range of within-individual variation in testes size than pure-species males. Moreover, although hybrids produced similar numbers of early stage sperm cells, hybrids produced significantly fewer mature spermatozoids than pure-species males. Interestingly, an introgressed individual produced numbers of live sperm comparable to pure-species males, but the majority of these sperm cells were abnormally shaped and non-motile. These results indicate that hybrid incompatibilities in late sperm development serve as a reproductive isolating barrier between species. The nature of this breakdown highlights the possibilities that hybrid males may vary in fertility and that fertility could possibly be recovered in introgressed males.

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Histological aspects and structural characteristics of the testes of Dendropsophus minutus (Anura, Hylidae)

Cited by 21 publications

References 16 publications

Influence of rainfall and temperature on the spermatogenesis of Leptodactylus macrosternum (Anura: Leptodactylidae)

Influence of rainfall and temperature on the spermatogenesis of Leptodactylus macrosternum (Anura: Leptodactylidae)

Histology and structure of the testicles in three species of Atelopus frogs (Anura: Bufonidae) endemic to the Sierra Nevada de Santa Marta, Colombia

Failed sperm development as a reproductive isolating barrier between species

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