Histological and histochemical study of the duodenum of the plains viscacha (<i><scp>L</scp>agostomus maximus</i>) at different stages of its ontogenetic development

Hoz, María Florencia Tano de la; Flamini, Mirta Alicia; Díaz, Alcira Ofélia

doi:10.1111/j.1463-6395.2012.00577.x

Cited by 11 publications

(25 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HID-AB pH 2.5 staining indicates that the degree of sulfation decreases from to the deeper adenomeres towards the common excretory duct of glands. Differential sulfation among Brunner's adenomeres is reported also by Jennings and Florey (1956) in the Guinea-pig and by Tano de la Hoz et al (2014) in the plains viscacha (belonging to the family Chinchillidae, next to the Guinea-pig family, Caviidae). Since desulfation increased binding of SNA and SBA lectins, it can be supposed that sulfated groups are linked to sialic acid and GalNAc, respectively.…”

Section: Speciesmentioning

confidence: 91%

“…The overgrowth of duodenal microflora is limited by the gastric juice (e.g., Fried et al, 1994), thus acidity of Brunner's gland secretion could be involved in this regulation. It is not clear why the sulfation of mucins decreases from the deeper to the more superficial adenomeres, and if it depends on different functional stages of a same cell or distinct cell subpopulations (Tano de la Hoz et al, 2014). In any case, the Guinea-pig's Brunner glands seem capable to modulate the relative amounts of sulfated and sialylated residues in their secretion, possibly in relation to physiological or pathological conditions.…”

Section: Speciesmentioning

confidence: 92%

“…Suzaki and Kataoka (1992) by an ultrastructural study and a gold-lectin binding in the Golgi complex (in which O-linked glycosylation of mucins takes place) of the mouse came to similar conclusions. Some data are available also from conventional and lectin histochemistry in light microscopy (e.g., Krause, 2000;Pedini et al, 2001;Schumacher et al, 2004;Scillitani et al, 2007;Tano de la Hoz et al, 2014), from which a higher diversity of residues emerges in respect to the other secreting cells of the luminal epithelium. Little is known about the composition and distribution of the acidic residues in the oligosaccharidic chains, in particular about side O-acetylation of sialic acid.…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Comparative glycopattern analysis of mucins in the Brunner's glands of the guinea-pig and the house mouse (Rodentia)

Scillitani

Mentino

2015

Acta Histochemica

View full text Add to dashboard Cite

Section: Speciesmentioning

confidence: 91%

Section: Speciesmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Comparative glycopattern analysis of mucins in the Brunner's glands of the guinea-pig and the house mouse (Rodentia)

Scillitani

Mentino

2015

Acta Histochemica

View full text Add to dashboard Cite

“…Mucus, the product of the hydratation of mucins, is a common feature in the tunica mucosa of vertebrates; it is secreted by the goblet cells or mucous cells (MC) (Yashpal, Kumari, Mittal, & Mittal, 2007;Díaz, García, Figueroa, & Goldemberg, 2008a;Díaz, García & Goldemberg, 2008b;Tano de la Hoz, Flamini, & Díaz, 2012). These cells secrete a variety of mucins that contribute to the generation of a gelatinous layer, to protect and lubricate the intestinal surface to facilitate the transport of food and reduce the epithelial mechanical stress.…”

mentioning

confidence: 99%

Histochemical differences along the intestine of Corydoras paleatus (Siluriformes: Callichthyidae)

Plaul

Barbeito

Díaz

2016

RBT

View full text Add to dashboard Cite

Abstract:The Neotropical catfish Corydoras paleatus is a facultative air-breather and the caudal half of the intestine is involved in gas exchange. In South America, air-breathing fishes are found in tropical or sub-tropical freshwaters where the probability of hypoxia is high. The aim of this study was to characterize by traditional histochemical and lectinhistochemical methods the pattern of carbohydrate in the intestinal mucosa. Intestine samples were taken from 25 healthy adult specimens collected in Buenos Aires (Argentina). Samples were fixed by immersion in 10 % buffered formalin and routinely processed and embedded in paraffin wax. Subsequently, these sections were incubated in the biotinylated lectins battery. Labeled Streptavidin-Biotin (LSAB) system was used for detection, diaminobenzidine as chromogen and haematoxylin as a contrast. To locate and distinguish glycoconjugates (GCs) of the globet cells, we used the following histochemical methods: PAS; PAS*S; KOH/ PA*S; PA/Bh/KOH/PAS; KOH/PA*/Bh/PAS; Alcian Blue and Toluidine Blue at different pHs. Microscopically, the general structure of vertebrate intestine was observed and showed all the cell types characteristic of the intestinal epithelium. The cranial sector of catfish intestine is a site of digestion and absorption and its structure is similar to other fish groups. In contrast, enterocytes of the caudal portion are low cuboidal cells; and between these, globet cells and capillaries are observed, these latter may reach the mucosal lumen. Underlying the epithelium, observed a well-developed lamina propria-submucosa made of connective tissue; this layer was highly vascularized and did not exhibit glands. According to histochemistry, the diverse GCs elaborated and secreted in the intestine are associated with specific functions in relation to their physiological significance, with special reference to their role in lubrication, buffering effect and prevention of proteolytic damage to the epithelium together with other biological processes, such as osmoregulation and ion exchange. The lectinhistochemical analysis of the intestinal mucosa reveals the presence of terminal residues of glucose, mannose and galactose. In conclusion, this study has shown that GCs synthesized in the intestine of C. paleatus exhibit a high level of histochemical complexity and that the lectin binding pattern of the intestinal mucosa is characteristic of each species and the variations are related with the multiple functions performed by the mucus in the digestive tract. The information generated here may be a relevant biological tool for comparing and analyzing the possible glycosidic changes in the intestinal mucus under different conditions, such as changes in diet or different pathological stages. Rev. Biol. Trop. 64 (1): 327-340. Epub 2016 March 01.

show abstract

“…The results were evaluated by independent observers using a semi‐quantitative scale to determine the intensity of the reactions (0, negative reaction; 1, weak positive reaction; 2, moderate positive reaction, and 3, strong positive reaction). This classification was established according to previous histochemical studies (Carlucci et al, 2019; Cohen et al, 2014; Scillitani & Mentino, 2015; Tano de la Hoz, Flamini, & Díaz, 2014, 2016).…”

Section: Methodsmentioning

confidence: 99%

Development of the digestive system of Argentine hake, Merluccius hubbsi, larvae

Cohen

Diaz

Díaz

2020

Journal of Morphology

View full text Add to dashboard Cite

The Argentine hake Merluccius hubbsi is an important fishery resource of the Southwestern Atlantic Ocean and it is also a potential species for cultivation. In this work,the digestive system development in field-caught hake larvae was studied using histological and histochemical approaches. The digestive tract of larvae was divided into: oropharyngeal cavity (OPC), esophagus, stomach (that develops in the preflexion stage), and intestine. The annexed digestive glands consisted of the liver and the exocrine pancreas. At the beginning of the preflexion stage, teeth were developed in the OPC. There were mucous cells in the esophagus secreting different glycoconjugates from hatching. The enterocytes in the posterior intestine exhibited supranuclear vesicles associated with protein absorption. Mucous cells were observed in the posterior intestine in the preflexion stage and, in the anterior region, ending the flexion stage. Each type of glycoconjugates has a specific role. Acidic mucins lubricate and protect from mechanical damage, sialomucines protect from bacterial infections and neutral mucins regulate the acidity of mucus secretion, protect against abrasion and participate in the formation of the chyme, indicating a pregastric digestion. The liver was present since hatching with pancreatic tissue inside and increased in size acquiring the typical structure with hepatocyte cords, sinusoids, vacuoles, and hepatic duct. The hepatocytes vacuolization increased with larval development. The pancreas became extra-hepatic, with basophilic acinar cells and acidophilic zymogen granules. Throughout the ontogeny, the increased structural and functional complexity of the digestive system reflected the transition to exogenous feeding and nutritional increasing needs. K E Y W O R D S histochemistry, histology, ontogeny

show abstract

Histological and histochemical study of the duodenum of the plains viscacha (Lagostomus maximus) at different stages of its ontogenetic development

Abstract: María Florencia Tano de la Hoz, Mirta Alicia Flamini, and Alcira Ofelia Díaz, 2014. Histological and histochemical study of the duodenum of the plains viscacha (Lagostomus maximus) at different stages of its ontogenetic development.-Acta Zoologica (Stockholm) 95: 21-31.

Cited by 11 publications

References 38 publications

Comparative glycopattern analysis of mucins in the Brunner's glands of the guinea-pig and the house mouse (Rodentia)

Comparative glycopattern analysis of mucins in the Brunner's glands of the guinea-pig and the house mouse (Rodentia)

Histochemical differences along the intestine of Corydoras paleatus (Siluriformes: Callichthyidae)

Development of the digestive system of Argentine hake, Merluccius hubbsi, larvae

Contact Info

Product

Resources

About