Histological and Functional Changes in the Thyroid Glands of Mice Implanted With Hybridomas Secreting Monoclonal Autoantibody Against Mouse Thyroglobulin

Abstract

Mouse hybridoma cells secreting monoclonal antibody (mAb) against mouse thyroglobulin were established. The implantation of the hybridomas succeeded to induce high titers of circulating mAb against thyroglobulin in sera of mice. By using the implantation of the hybridomas in mice, the effect of autoantibody on the thyroid glands was studied histologically and functionally. In these mice the thyroid follicles were significantly swollen and warped, whereas there was no infiltration of inflammatory cells. The 125I-uptake in their thyroid glands was markedly decreased. There were no functional changes in control mice implanted with non-secreting P3U1 partner cells. Therefore, it was suggested that high titers of anti-thyroglobulin autoantibody could definitely cause the histological and functional damages in the thyroid glands.

Production of Experimental Autoimmune Sialadenitis in Mice Immunized with Homologous Salivary Gland Extract andKlebsiella O3 Lipopolysaccharide

“…Our experimental system using KO3 LPS might also provide a new model for autoimmune sialadenitis. A series of experimental autoimmune diseases that have been established by using KO3 LPS adjuvant were characterized by the extensive infiltration of polymorphonuclear leukocytes, suggesting the participation of Arthus reaction [15,21,22,26,35]. In the present study, however, mononuclear cells consisting mainly of small lymphocytes were found at the early stage after immunization and maintained until the late stage.…”

“…The intensity of the adjuvanticity of KO3 LPS was much stronger than that of complete Freund's adjuvant [13,15,16]. Utilizing the potent KO3 LPS adjuvant, we have established a series of experimental models for various autoimmune diseases, such as ophthalmitis [17,19], thyroiditis [13,16,[20][21][22], pancreatitis [23], hepatitis [24], hemolytic anaemia [25], adrenalitis [26], myocarditis [27], orchitis [28,29], enterocolitis [30] and arthritis [31] in mice. In the present study, we tried to establish experimental murine model for autoimmune sialadenitis by using KO3 LPS adjuvant.…”

Production of Murine Collagen‐Induced Arthritis Using Klebsiella pneumoniae O3 Lipopolysaccharide as a Potent Immunological Adjuvant

“…The intensity of the adjuvanticity of KO3 LPS was much stronger than that of complete Freund's adjuvant (13-15). Utilizing the potent KO3 LPS adjuvant, we have established a series of experimental models for various autoimmune diseases, such as ophthalmitis (8,27,35), thyroiditis (14,15,30,(33)(34)(35), pancreatitis (26), hepatitis (11), hemolytic anemia (10), adrenalitis (6), myocarditis (9), orchitis (7, 29), and enterocolitis (17) in mice. In the present study, we tried to establish CIA for autoimmune RA by using KO3 LPS adjuvant.…”

Experimental Murine Model for Autoimmune Enterocolitis Using Klebsiella pneumoniae O3 Lipopolysaccharide as a Potent Immunological Adjuvant

“…The intensity of the adjuvanticity of KO3 LPS was much stronger than that of complete Freund's adjuvant (21)(22)(23)38). Utilizing the potent KO3 LPS adjuvant, we have established a series of experimental models for various autoimmune diseases, such as ophthalmitis (14,32,40), thyroiditis (23,24,35,(38)(39)(40), pancreatitis (31), hepatitis (18), hemolytic anemia (16), adrenalitis (9), myocarditis (15), and orchitis (10,34) in mice. In the present study, we tried to establish a murine experimental model for autoimmune enterocolitis by using KO3 LPS adjuvant.…”