Experimental Murine Model for Autoimmune Enterocolitis Using <i>Klebsiella pneumoniae</i> O3 Lipopolysaccharide as a Potent Immunological Adjuvant

Paeng, Noriko; Morikawa, Akiko; Kato, Yasuhiro; Sugiyama, Tsuyoshi; Koide, Naoki; Yoshida, Tomoaki; Yokochi, Takashi

doi:10.1111/j.1348-0421.1999.tb02371.x

Cited by 10 publications

(5 citation statements)

References 33 publications

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“… 20,21 It was previously demonstrated that LPS or its lipid A portion enhanced MRL/lpr nephritis in mice, 5 experimental autoimmune uveitis, 6 experimental autoimmune myocarditis 7 and experimental autoimmune enterocolitis. 8 However, few studies clearly showed a role for LPS in autoimmune arthritis.…”

Section: Discussionmentioning

confidence: 99%

Bacterial lipopolysaccharide acts as an adjuvant to induce autoimmune arthritisin mice

2000

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SUMMARYWe investigated the ability of lipopolysaccharide (LPS) as an adjuvant to induce autoimmune arthritis. LPS from Escherichia coli was intraperitoneally injected into DBA/1J mice together with the joint cartilage component type II collagen (CII) on day 0. Thereafter, the injection of CII and LPS was continued every 2 weeks up to day 56. The results showed that mice injected with CII plus LPS had signs of arthritis on day 55 and the joint in¯ammation reached a peak on day 75. Injection of CII or LPS alone induced no arthritis. Histologically, marked oedema of synovium and intense in®ltration of in¯ammatory cells, including neutrophils, were observed 3 days after the onset of joint in¯ammation. Twenty-one days later, there were marked proliferation of synovial tissues with many mononulear cells and destruction of cartilage. Anti-CII immunoglobulin G (IgG) and IgG2a antibodies were markedly produced in mice injected with CII plus LPS. Pronounced secretion of cytokines, including interleukins-12 and -1b, interferon-c and tumour necrosis factor-a, was also observed in these animals. Arthritis was passively transferred into naive syngeneic mice with sera but not with lymphoid cells from mice given CII with LPS. Other types of LPS from Salmonella enteritidis, Salmonella typhimurium and Klebsiella pneumoniae as well as lipid A from E. coli, induced in¯ammation in joints when administered with CII. Polymixin B sulphate mixed with LPS or lipid A blocked the induction of joint in¯ammation. These results indicate that LPS appears to play an important role as an adjuvant in the induction of arthritis in which autoimmunity to CII is involved.

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Section: Discussionmentioning

confidence: 99%

Bacterial lipopolysaccharide acts as an adjuvant to induce autoimmune arthritisin mice

2000

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“…Collagen-induced enterocolitis [42] as well as arthritis [43] has been observed in experimental animals when immunized with homologous colonic extracts and collagen type II together with Klebsiella lipopolysaccharides.…”

Section: Aetiopathogenesismentioning

confidence: 99%

The Role ofKlebsiellain Crohn’s Disease with a Potential for the Use of Antimicrobial Measures

Rashid

Ebringer

Wilson

2013

International Journal of Rheumatology

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There is a general consensus that Crohn's disease (CD) develops as the result of immune-mediated tissue damage triggered by infections with intestinal microbial agents. Based on the results of existing microbiological, molecular, and immunological studies, Klebsiella microbe seems to have a key role in the initiation and perpetuation of the pathological damage involving the gut and joint tissues in patients with CD. Six different gastroenterology centres in the UK have reported elevated levels of antibodies to Klebsiella in CD patients. There is a relationship between high intake of starch-containing diet, enhanced growth of gut microbes, and the production of pullulanases by Klebsiella. It is proposed that eradication of these microbes by the use of antibiotics and low starch diet, in addition to the currently used treatment, could help in alleviating or halting the disease process in CD.

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“…Increased levels of LPS are observed in obesity and other metabolic disorders as well as adipose tissue inflammation and pancreatic beta-cell dysfunction. For example, LPS has been shown to aggravate experimental autoimmune myocarditis (Kato et al, 1993), EAU (Yokochi et al, 1993) and experimental autoimmune enterocolitis (Paeng et al, 1999). In addition, LPS appears to play an important role as an adjuvant in the induction of autoimmune arthritis in a mouse model (Yoshino et al, 2000).…”

Section: The Role Of Microbial Metabolitesmentioning

confidence: 99%

The role of the gut microbiome in eye diseases

Zysset-Burri

Morandi

Herzog

et al. 2023

Progress in Retinal and Eye Research

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Experimental Murine Model for Autoimmune Enterocolitis Using Klebsiella pneumoniae O3 Lipopolysaccharide as a Potent Immunological Adjuvant

Cited by 10 publications

References 33 publications

Bacterial lipopolysaccharide acts as an adjuvant to induce autoimmune arthritisin mice

Bacterial lipopolysaccharide acts as an adjuvant to induce autoimmune arthritisin mice

The Role ofKlebsiellain Crohn’s Disease with a Potential for the Use of Antimicrobial Measures

The role of the gut microbiome in eye diseases

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