Histological and cytological studies on the developing thymus of sharpsnout seabream, <i>Diplodus puntazzo</i>

Romano, Nicla; Fanelli, Monica; Papa, Giovanni; Scapigliati, Giuseppe; Mastrolia, Lucia

doi:10.1046/j.1469-7580.1999.19410039.x

Cited by 25 publications

(35 citation statements)

References 22 publications

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“…In addition, outer and inner RECs embraced capillaries and lined the capsule and trabeculae. Thus, RECs in turbot defined a barrier with the rest of the organism, establishing a microenvironment for thymocyte differentiation, as has been suggested in other species such as rainbow trout ), sharpsnout seabream (Romano et al 1999a) and common carp (Romano et al 1999b). …”

Section: Discussionmentioning

confidence: 88%

“…The morphology and cellular composition of fish thymus vary greatly, especially their epithelial components (Gorgollon 1983;Castillo et al 1990;Chilmonczyk 1992;Romano et al 1999a;Xie et al 2006;Mohammad et al 2007). Reticulo-epithelial cells (RECs) have been described and classified mainly by means of transmission electron microscopy.…”

Section: Introductionmentioning

confidence: 96%

“…These cell types establish a meshwork in which parenchymal cells rest and thus RECs create an appropriate microenvironment for thymocyte differentiation (Savino and Dardenne 2000). Depending on the studied species, one [Rutilus rutilus (Zapata 1981)], three [Sicyases sanguineus (Gorgollon 1983)], four [(Diplodus puntazzo (Romano et al 1999a)] and up to five [Oncorhynchus mykiss )] RECs subtypes have been reported in the fish thymus.…”

Section: Introductionmentioning

confidence: 99%

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Morphological and immunohistochemical characterisation of the thymus in juvenile turbot (Psetta maxima, L.)

Vigliano

Losada

Castello³

et al. 2011

Cell Tissue Res

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We performed structural and immunohistochemical studies on the thymus of juvenile turbot (Psetta maxima L.) in order to define its cellular composition. The thymic stroma was mainly composed of two subpopulations of reticulo-epithelial cells (RECs). RECs immunoreactive to anti-actin antibody were distributed through the organ, while RECs that were cytokeratin-immunopositive were located in the outer zone of the thymus. The parenchyma of the thymus was composed of several cell types such as lymphocytes/thymocytes, lymphoblasts, melano-macrophages and to a lesser extent of nurse-like cells, immunoglobulin positive (Ig+) cells, mucous cells, rodlet cells and neuroendocrine cells. CD3ε+ lymphocytes were mainly located in the outer zone. On the other hand, Ig+ cells were observed in the transitional region between the inner and outer zones of the thymus. The neuroendocrine cells were large and exhibited immunoreactivity to neuropeptide Y and vasoactive intestinal polypeptide. They were located in the inner zone of the thymus in close association with lymphoblasts and lymphocytes/thymocytes. This work provides useful information on the structure and cellular composition of the thymus of turbot, identifying several immunomarkers that allow the identification of different cell types, providing the basis for further studies on the immune response of turbot against diseases.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Morphological and immunohistochemical characterisation of the thymus in juvenile turbot (Psetta maxima, L.)

Vigliano

Losada

Castello³

et al. 2011

Cell Tissue Res

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“…Diferencias morfológicas entre timocitos, donde los corticales fueron más pequeños que los medulares, se describen en otras especies como los humanos (Junquera et al, 1988) y la brema del mar (Romano et al, 1999).…”

Section: Discussionunclassified

“…Allí, las células linfoides de la línea T puedan desarrollar, proliferar, madurar, generar su repertorio de receptores de antígenos, y dejar este órgano para poblar órganos linfoides secundarios (Romano et al, 1999). La estructura histológica del timo y la ontogenia del sistema inmune ha sido estudiada y establecida en la mayoría de especies domésti-cas (Osburn et al, 1982;Tizard, 2002); sin embargo, no se dispone de estudios en fetos de camélidos sudamericanos.…”

Section: Introductionunclassified

Caracterización Histológica Del Timo en Fetos De Alpaca

Sandoval

Chavera

et al. 2012

Rev. investig. vet. Perú

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The objective of the present study was to study the cytoarchitectural structure of the cervical and thoracic segments of thymus in alpaca (Lama pacos) fetuses. For this purpose, 10 fetuses between 40-190 days of gestation were collected form the local slaughterhouse. At day 40, the developing thymus has acinar cytoarchitectural morphology, infiltrated by a few lymphoid cells surrounded by mesenchymal cells. At day 60, the cervical segment looks like a «follicular» lymphoid, densely populated by thymocytes, whereas the thoracic region has a cystic appearance with short trabecular projections. Cortex and medulla differentiation starts in the posterior cervical and thoracic segments by day 100 and in the anterior cervical segments by day 130, and is well established by day 165. The thymic capsule remained as a double cellular layer of lax connective tissue. Subcapsular and trabecular blood vessels have fenestrated endothelium. Erythroblastic cells were observed by day 100 in the cortex and subcapsule zones. Medullar Hassall's corpuscles were observed by day 100, and the epithelial reticular cell barrier in the cortical medullar zone was observed by day 130. Key words: alpaca, fetus, thymus, Hassal corpuscle RESUMENEl objetivo del presente trabajo fue el estudio citoarquitectural de los segmentos cervicales y torácicos del timo en fetos de alpaca (Lama pacos). Se recolectaron 10 fetos entre 40 y 190 días de desarrollo para ser sometidos a estudio histológico. A los 40 días, el esbozo del timo tiene una morfología citoarquitectural de tipo acinar infiltrado por escasas células linfocíticas y rodeado por células mesenquimales. A los 60 días, el segmento cervical se presenta como un "folículo" linfoide densamente poblado por timocitos, mientras que la región torácica tiene una apariencia cavitaria con proyecciones trabeculares cortas. La diferenciación de corteza y médula se inicia en los segmentos cervicales posteriores y torácicos a los 100 días y en los segmentos cervicales anteriores a los 130 días. Todos los segmentos presentan disposición lobulillar bien definidos en corteza y médula a los 165 días. La cápsula tímica se mantiene principalmente como una doble capa celular con tejido conjuntivo laxo. Los vasos sanguíneos subcapsulares y trabeculares presentan endotelio fenestrado. Las células eritroblásticas se ubican en las zonas subcapsulares

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Majority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.)

et al. 2007

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Real-time polymerase chain reaction (PCR) and in situ hybridization analyses were performed to investigate the occurrence and distribution of T-lymphocytes expressing TcRbeta in intestine and lymphoid tissues of the bony fish, Dicentrarchus labrax (sea bass). Immunohistochemistry with the monoclonal antibody DLT15 (pan-T-cell marker) was carried out to compare the cytology, distribution and number of T-cells and TcRbeta+ cells in the various sampled lymphoid organs. The highest TcRbeta expression was revealed by real-time PCR in the thymus, with high levels also being found in the gut. In the thymus, DLT15+ and TcRbeta+ cell populations were concentrated in the cortex and TcRbeta+ cells were notably reactive at the cortical-medullary border, suggesting a specialized role of this region in thymocyte selection. The density of DLT15+ T-cells increased from the anterior to posterior intestine, whereas TcRbeta+ lymphocytes were more numerous in the middle intestine compared with other segments. The existence, in fish thymus, of a medulla and a cortex comparable with those of mammals is revealed by this study. The concentration of TcRbeta+ cells in the sea bass midgut also strongly suggests a special role of this intestinal segment in antigen-specific cellular immunity. The large population of TcRbeta(-)/DLT15+ T-cells in the posterior gut can probably be ascribed to the TcRgammadelta phenotype fraction.

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Histological and cytological studies on the developing thymus of sharpsnout seabream, Diplodus puntazzo

Cited by 25 publications

References 22 publications

Morphological and immunohistochemical characterisation of the thymus in juvenile turbot (Psetta maxima, L.)

Morphological and immunohistochemical characterisation of the thymus in juvenile turbot (Psetta maxima, L.)

Caracterización Histológica Del Timo en Fetos De Alpaca

Majority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.)

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